Aerobic gram pos bacilli part 2

Corynebacterium Animal, plant, and human pathogen

Nm Motility Corynebacterium
- Capsule Corynebacterium
Pleomorphic rods palisades Corynebacterium micro
Mycobacteria nocardia Corynebateria are closely related to
these families
Mesodiaminopimelic acid m-dap Cell wall of corynebacteria contains __
mycolic acid as opposed to mycobacteria and
nocardia which have __ in cell wall
Lipophilic nonlipophilic Corynebacterium groups
Lipophilic Corynebacterium Addition of lipids in the culture medium
will enhance this coryne group
c. jeikeium c. urealyticum Lipophilic bacteria example
c. diphtheriae Diphtheria bacillus
c. diphtheria Kleb loefflers bacillus
Kleb loefflers bacillus diphtheria bacillus Other terms for c. diptheriae
Aerobe Facultative anaerobe Oxygen requirement c. diphtheria
Nasopharynx Carriers of c. diphtheria have this
bacterium in what part of their body
Droplets direct contact hand-mouth MOT c. diphtheria
+ Catalase c. diphtheria
- Urease c. diphtheria
+ Nitrate to nitirite c. diphtheria
- Gas c. diphtheria
Serum cysteine k tellurite Enrichments for c. diphtheria growth
15-40C Temp at which c. diphtheria grows
F c. diphtheria are heat stable organisms
T c. diphtheria are dry-resistant
organisms
Diphtheria toxin Virulence factor c. diphtheria
Enzymatic a substance Diphtheria toxin is like this substance
from v. choleras choleragen
130 ng/kg bw Lethal dose of c. diphtheriae
Diphtheria Contagious disease characterized by
pseudomembranous formation in the
throat
Immunization dpt Diphtherias only effective control is
Diphtheria toxin Inhibits protein synthesis in eukaryotic
cells is caused by this toxin
Lyogenic bacteriophage Carries the tox gene
Trypsinazation Mechanism in which diphtheria toxin
becomes toxic
Trypsin Diphtheria toxin is nontoxigenic until
exposed to
A and b Trypsinaztion cleaves diph toxin into
fragments
Diphtheria toxin Toxin which causes pseudomembrane
formation on the tonsils, larynx, and
pharynx
7.8-8.0 aerobe iron Diphtheria toxin production in vitro
requires this ph, oxygen environment,
and enrichment
Respiratory cutaneous 2 forms of diphtheria
Cardiac failure Death in respiratory diphtheria
Velat sore barcoo rot Other terms for cutaneous diphtheria
Cutaneous diphtheria Diphtheria form with less complication
Slow healing ulcers Cutaneous diphtheria is characterized
by
c. ulcerans c. pseudotuberculosis c. diphtheria group members
c. diphteriae Chinese letters arrangement
Xvyl Letters resembling c. diphtheria
morphology
Methylene blue c. diphtheria may appear club shaped
swellings and beaded forms in this stain
Club shaped swellings beaded forms Methylene blue may make s. diphtheria
appear like these
Lamb neissers alberts ljubinsky Stains for c. diphtheriae chromatic
granules
Bg Hemolytic patterns c. diphtheria
Loeffler slant cysteine tellurite blood Primary inoculation on __ and
agar subculture on __ may improve c.
diphtheriae
Rabbit serum For lipophilic coryneforms, broths are
added with
15-40c Temp at which c. diphtheriae
multiplication takes place
Iron Addition of __ to iron-starved cultures
inhibits diph toxin immediately
Ctba Mod. Of tinsdale medium
Tinsdale medium Ctba is a mod of this medium
Cysteine tellurite blood agar Ctba meaning
Ctba Preferred medium for isolation and
identification of corynebacteria
Sheep blood bovine serum cysteine k Ctba contains
tellurite
Black or brown colonies with brown halo + ctba c. diphtheria
Staph strep Pther bacteria with black colonies on
ctba
Mitis intermedius gravis 3 colonial types of c. diphtheria
Mitis Fried-egg colony on bap c. diphtheria
Bleach Mitis c. diphtheria odor
Mitis Small, black, convex colonies c.
diphtheria
Intermedius Very small, flat, dry, grayish black
 colonies
- Hemolytic pattern intermedius c.
diphtheria
Gravis Large, flat, dark gray, daisy head c.
diphtheria
Gravis Daisy head colonies, non-hemolytic c.
diphtheria
Na thiosulfate Additional enrichment in tinsdale agar
based from ctba
Tinsdale agar Na thiosulfate added to this medium
basing from ctba
Black colonies with brown halo + result tinsdale agar c. diphtheria
Pais slant Other term for loeffler serum slant
Loeffler serum slant Pais slant also known as
Poached egg colonies Pais slant + result c. diphtheria
Loefflers serum slant Useful for observing microscopic
morphology and metachromatic
granules c. diphtheria
Babes Ernst Metachromatic granules inside c.
diphtheria
Christen urea slant Slant used for c. dphtheriae to observe
urease production of c. urealyticum
c. urealyticum Corynebacterium which is urease
positive
Schicks test Skin test for c. diphtheria
Immunodiffusion guinea pig lethal test Toxigenicit tests for c. diphtheria
tissue culture pcr
Elek plate test In vitro immunodiffusion test for c.
diphtheria
35C 18 24 48 hrs Elek test temp and time
4-5mm fine precipitin lines at 45 degree + result for elek test
angle to the streaks
Elek test guinea pig lethal test tissue Tests for diphtheria anti-toxin
culture
Toxin gene Pcr detects __ for c. diphtheria
Hoffmans bacillus c. pseudodiphtheriticum common name
c. pseudodiphtheriticum Hoffmans bacillus
Nasopharynx c. pseudodiphtheriticum is a normal
flora of what part
Parallel rows Palisades arrangement characteristic
No pleomorphism c. pseudodiphtheriuiticum micro
+ Urease c. pseudodiphtheriticum
+ Nitrate production c.
pseudodiphtheriticum
Inguinal axillary rectal c. jeikeium is a common skin flora in
these parts of the body of hospitalized
individuals
Obligate aerobe Oxygen requirement c. jeikeium
+ Urease c. jeikeum
+ Nitrate to nitrite c. jeikeium
c. jeikeium Corynebacterium associated with
history of iv drug abuse
c. jeikeium Common cause of diphtheroid
prosthetic valve endocarditis
c. jeikeium Corynebacterium which is multiple
antibiotic resistant
Pleomorphich club shaped v forms Micro c. jeikeium
- Hemolytic pattern c. jeikeium
1% tween 80 Culture c. jeikeium appear as large
colonies when supplemented with
Mastitis c. ulcerans causes __ in cattle and other
domestic animals
c. ulcerans Cause of mastitis in cattles and other
domestic animals
Animal contact ingestion of MOT c. ulcerans
unpasterurized dairy products
c. ulcerans Diphtheria-like sore throat
Skin ulcers exudative pharyngitis c. ulcerans has been isolated from __
and __
Narrow b Hemolytic pattern c. ulcerans
Brown halo + result c. ulcerans ctba
+ Gelatin formation c. ulcerans
- Nitrate to nitrite c. ulcerans
Animal contact MOT c. Granulomatous lymphadenitis is caused
pseudotuberculosis by
Dermonecrotic toxin c. pseudotuberculosis releases this
toxin which causes death of a variety of
cell types
Diphtheria toxin Another toxin which can be produced
by c. pseudotuberculosis
+ Urease c. pseudotb
- Gelatin c. pseudotb
c. urealyticum Corynebacterium which is considered
as a urinary pathogen
Obligate aerobe Oxygen requirement c. urealyticum
- Hemolytic pattern c. urealyticum
- Glucose c. urealyticum
- Maltose c. urealyticum
+ Catalase c. urealyticum
+ Urease c. urealyticum
Diphtheria toxin Toxin produced by c. urealyticum when
infected with toxin-carrying
bacteriophage
c. ulcerans c. pseudotuberculosis c. Urease positive corynebacteria
urealyticum c. pseudodiphtheriticum
c. diphtheriae c. pseudodiphtheriticum Nitrate producer corynebacteria
- Esculin hydrolysis corynebacteria
c. ulcerans The only gelatinase + corynebacteria
c. diphtheria c. ulcerans c. Ctba halo producing corynebacteria
pseudotuberculosis