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American Society of
Agricultural and Biological Engineers
ASABE is a professional and technical organization, of members worldwide, who are dedicated to advancement of
engineering applicable to agricultural, food, and biological systems. ASABE Standards are consensus documents
developed and adopted by the American Society of Agricultural and Biological Engineers to meet standardization
needs within the scope of the Society; principally agricultural field equipment, farmstead equipment, structures, soil
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ANSI/ASAE S459 MAR1992 (R2012)
Approved February 1993; reaffirmed February 2012 as an American National Standard
1 Purpose
1.1 This Standard is designed for use in determining the mechanical
properties of animal bones such as the ultimate shear strength, ultimate
bending strength, apparent modulus of elasticity, and fracture energy.
2 Scope
2.1 Shear and bending tests of intact animal bones provide an objective
method for evaluating the effects of age, sex, nutrition, contaminants,
and environment on the physical condition of the animal.
2.2 Understanding the problems encountered in evaluating the
mechanical properties of animal bones and attempting to compare the
results of previous investigators, has led to the need for a standard
procedure for testing, data interpretation, and reporting of results.
2.3 The type of test selected, shear or three-point bending, will be Figure 1 Force deformative curve
dependent on the size and shape of the bone. The three-point bending
tests should be used only when the bone is straight, has a symmetrical
cross section, and has a support length to diameter ratio greater than 10. 3.9 Apparent modulus of elasticity: When a material is inelastic
The shear test is good for any size or shape of bone. (bone), loading and unloading the material several times within the linear
2.4 Determination of the shear or bending properties of animal bone limit may produce loading and unloading curves that may give different
requires the development of a force-deformation curve. From a shear values for the modulus of elasticity. The apparent modulus of elasticity is
force-deformation curve, ultimate shear force, ultimate shear strength the value of the modulus calculated from the first loading cycle (see
(stress), and fracture energy can be obtained. From a bending force- ASAE Standard S368, Compression Test of Food Materials of Convex
deformation curve, ultimate bending force, deformation to fracture, Shape).
ultimate bending strength (stress), apparent modulus of elasticity, and
fracture energy can be obtained. Any of these mechanical properties can
be used for the purpose of evaluation, and it is recommended that more 4 Apparatus
than one property be used. 4.1 Testing machine. Any testing machine capable of applying a
constant rate of crosshead movement with the following:
4.1.1 A driving device for the crosshead with a reproducible speed with
3 Definitions and accuracy of 1.0%.
3.1 Force: Fracture load applied to the test specimen.
4.1.2 A force-deformation indicating mechanism (x y plotter, chart
3.2 Deformation: Amount specimen deflects under load. recorder, data acquisition unit, etc.). The mechanism must be calibrated
3.3 Force-deformation curve: A graph (see Fig. 1) with values of before testing. The testing machine should be periodically verified
deformation on the abscissa and values of force on the ordinate. following ASTM Standard E-4, Practices for Load Verification of Testing
3.4 Ultimate shear strength: Maximum shear stress that can be Machines.
sustained by a material before rupture caused by a shear load. 4.2 Shear testing fixture. A double shear block arrangement as shown
3.5 Ultimate bending strength: Maximum bending stress developed in in Fig. 2 should be used. The clearance between the 2 sample supports
a material before rupture caused by a flexural load. and the shear loading bar shall not exceed 0.05 mm. The radius of
curvature of the sample mounting blocks and the loading bar shall
3.6 Fracture energy: The energy required to deform a material to the depend on the size of the specimen.
point of fracture. It is the area under the force-deformation curve up to
the point of fracture (see Fig. 1). 4.3 Three-point bending fixture. The three-point bending test fixture as
shown in Fig. 3 with adjustable fulcra should be used. The 3 loading
3.7 Stress-strain diagram: Graph of stress as a function of strain which supports must be rounded to avoid damage to the specimen. A radius of
is constructed from data taken from the force-deformation curve. 4.0 mm is recommended for the 3 supports. The 2 fulcrum points should
3.8 Modulus of elasticity: The slope of the straight line portion of a be adjustable in order to obtain a support length to test specimen
stress-strain diagram. diameter ratio, greater than 10.
ANSI/ASAE S459 MAR1992 (R2012) Copyright American Society of Agricultural and Biological Engineers 1
Figure 2 Double shear block test fixture
8 Calculations
8.1 Force, F, and deformation, , to rupture. If a recording chart is
used, the value of the force is read directly from the chart, but the
deflection is determined by multiplying the chart reading by the
crosshead speed and dividing by the chart speed.
8.2 The ultimate shear strength (stress) is determined by:
F
2A
where
Figure 3 Three-point bending test fixture shear stress, Pa
ANSI/ASAE S459 MAR1992 (R2012) Copyright American Society of Agricultural and Biological Engineers 2
F applied fracture force, N d inside minor diameter, m
A initial cross-sectional area, m2 8.4 Apparent modulus of elasticity is calculated for three-point bending
8.3 The ultimate bending strength (stress) is calculated by: by:
FLC FL 3
E
4I 48I
where where
ultimate bending stress, Pa E apparent modulus of elasticity, Pa
C distance from neutral axis to outer fiber, m deformation, m
I moment of inertia, m4
L distance between supports, m
9 Report
F applied force, N
9.1 The final report shall include the following:
8.3.1 Most bone cross sections can be modeled as either a hollow
ellipse or a quadrant of an ellipse (see Fig. 4). The moment of inertia for 9.1.1 Date the test was performed.
a hollow ellipse is: 9.1.2 Complete identification of the bone tested including type, size,
shape, and previous history.
I 0.049 B D 3 b d 3 9.1.3 Complete identification of the test used with dimensions of the test
For a hollow ellipse: fixture, crosshead speed, load scale, and chart speed if used.
9.1.4 Method of placing specimen on test fixture.
D
C 9.1.5 Condition of the specimen when tested (fresh, frozen and thawed,
2 or cooked and dried).
For a quadrant of an ellipse: 9.1.6 Number of specimens used.
I 0.0549 B D 3 b d 3 9.1.7 Type of testing machine.
9.1.8 Calculated results and the type of statistical analysis used to
C 0.57559D
analyze the data.
where
B outside major diameter, m Cited Standards: Normative References
b inside major diameter, m ASTM E4, Load Verification of Testing Machines
D outside minor diameter, m ASAE S368, Compression Test of Food Materials of Convex Shape
Annex A for bone data criteria and comparison of bending and shear bone testing,
(informative) Journal of Animal Science 69:682693.
References Rowland, L. O., Jr., R. H. Harms, H. R. Wilson, E. M. Ahmad, R. W.
Waldroup, and J. L. Fry. 1988. Influence of various dietary factors on
Combs, N. R., E. T. Kornegay, M. D. Lindeman, D. R. Notter, J. H. bone fragility of caged layers. Poultry Science, 47:507511.
Wilson, and J. P. Mason. 1991. Calcium and phosphorus requirement of Wilson, J. H. and J. P. Mason. 1992. Bone breaking strength as influ-
swine from weaning to market weight. Development of response curves enced by preconditioning, Transactions of ASAE 35(1):263265.
ANSI/ASAE S459 MAR1992 (R2012) Copyright American Society of Agricultural and Biological Engineers 3