Procedure

Standard Preparation

About 10mg of quinine sulphate is obtained which is dissolved in 1 litre of

distilled water in a dark container to produce 10ppm solution. The 0.1M sulphuric
acid solution is made from stock solution of 18M in 250ml volumetric flask. The
standards are prepared in the following table in 25mL volumetric flask:

Standards (g/mL) Volume of 10ppm Volume of distilled Volume of 0.2N

quinine (mL) water (mL) sulphuric acid
(mL)
0.2 0.5 12.0
0.4 1.0 11.5
0.6 1.5 11.0 12.5
0.8 2.0 10.5
1.0 2.5 10.0
Total volume of each standard=25.0mL

Sample Preparation

The sample is prepared from 0.5mL of Livita energy drink with the addition of
12.0mL of distilled water and 12.5mL of 0.1M sulphuric acid in 25mL volumetric
flask. It is then tested with FES.