Introduction

Fluorescence is the emission of light from a substance that has absorbed

electromagnetic radiation or other light sources. In other word, it is a
luminescent without a change in spin multiplicity. By the action of molecules
being excited to higher energy level, the fluorescence is produced. It can be
described energy being absorbed and excited to higher energy level, followed by
vibrational relaxation, internal crossing, vibrational relaxation which leads to
fluorescence Normally, most substance absorbs energy from uv-region and then
emits light within visible region. In order to detect the level of fluorescence, an
electromagnetic spectroscopy called as fluorescence spectroscopy is used. It
uses a beam of light which is passes through a sample that have a selected
analyte, which is then excited by the absorption of the energy, thus causing
them to emit light. For this kind of spectroscopy, a certain analyte must have the
following characteristics; having benzene ring structure or conjugated double-
double bond as it is needed for the emission of fluorescence. Thus the sample
used in this experiment has the proper characteristic as latter and as such,
quinine in energy drinks are used as the analyte for easy to obtain and measured
using FES device due high quantum yield.

Purpose

To provide correlation of standards and to detect the concentration of quinine in

a given energy drink (Livita).