Introduction

The main difference between various chemicals is colour. The white light
composed of wide range of wavelength that is in ultraviolet, visible and infrared
region. Wavelength and frequency differs for each latter region. As light passes
through or bounce off from a coloured compound, certain amount of energy is
absorbed which all those coloured compound have similarity in conjugated
double-double bond structure such as alizarin and benzene. For this purpose,
ultraviolet-visible spectroscopy is used to measure the absorption within
ultraviolet-visible region with the usage of light from visible region or near-UV
light region. The absorption is affected by the colour or the presence of
conjugated double bond of a compound. During the absorption of UV light, the
molecules experiences electronic transition which quite similar to fluorescence
spectroscopy.

Purpose

To determine the concentration of iron in food sample (Honeystar Breakfast

cereal)