g (bacteria and molds) of soil given the following (5 pts.

):
1. Compute the cfu/100

10-5 10-6 10-7

Bacteria TNTC, TNTC 225, 245 20, 30

Molds 130, 145 10, 15 0, 0
For

bacteria use 25-250; for molds use 10-150

Volume used for plating: 0.1 ml

2. Compute the cfu/100 ml of milk given the following (7 pts):

TNTC, TNTC 265, 280 29, 31 0, 0 0, 0

3. Compute the bacteria/100 ml of milk given the following (3 pts.):

Cell count per field: 0, 0, 1, 5, 1, 0, 1, 0, 0, 2, 2, 1, 0, 1, 0, 0, 0, 0, 1, 1, 1, 2, 1, 0

Volume used: 0.01 ml

Dilution: 100
sample
4. Compute the cfu/g (lactic acid bacteria and coliforms) and the lactic acid content of burong mustasa
given the following (8 pts.) (MRS medium is for lactic acid bacteria; VRB or violet red bile agar is for coliform
counts):

1st sampling 2nd sampling

10-3 10-4 10-5 10-3 10-4 10-5
MRS 110, 120 9, 10 0, 0 TNTC, TNTC, 110, 100
TNTC TNTC
VRB 250, 240 0, 0 0, 0 25, 30 0, 0 0, 0
Vol. of 0.1N
NaOH used 10.0 40.0
(ml)
Vol. used for plating: 0.1 ml
Vol. of sample used for lactic acid content determination: 10 ml

5. Compute the titratable acidity of the vinegar sample if 4 ml of 1.0 N NaOH was used to titrate 50 ml of sample (2
pts). Can this sample be used commercially? (Commercial vinegar should have 4.0% titratable acidity)
6. Determine the Presumptive and Confirmed MPN of the following water samples (5 pts):
Deep Well no. 1
Presumptive Confirmed
50 ml 10 ml 1.0 ml 50 ml 10 ml 1.0 ml
No. of
1 5 5 1 5 5
tubes used
No. of
positive 1 5 5 1 3 1
(+) tubes
MPN/100
ml

Deep Well no. 2

Presumptive Confirmed
50 ml 10 ml 1.0 ml 50 ml 10 ml 1.0 ml
No. of
1 5 5 1 5 5
tubes used
No. of
positive 1 1 0 1 0 0
(+) tubes
MPN/100
ml

7. The same water samples used in #6 were also analyzed for total aerobic bacterial count. The following data were
obtained after spread plating on R2A agar medium.

Dilution Plate Count

Deep Well no. 1 Deep Well no. 2
Plate 1 Plate 2 Plate 3 Plate 1 Plate 2 Plate 3
10-1 TNTC TNTC TNTC TNTC TNTC TNTC
10-2 123 132 133 54 50 43

a. What is the cfu/ml of each of the two water samples? (5 pts)

b. Based on your computations in #6 and #7, which water source could you recommend for human
consumption? Explain your answer. (5 pts)

8. Given the following data:

Zone of Inhibition (in mm) against
Antibiotics
E. coli S. aureus P. aeruginosa
Ampicillin 10 15 0
Polymyxin 15 0 11
Streptomycin 15 15 15

a. Rank the antibiotics based on their efficacy against the test organisms (e.g. A<B<C). (3 pts)
b. General conclusion on the efficacy of the antibiotics (e.g. broad spectrum, narrow spectrum, etc.) (2 pts)

9. Given the following table:

Number of Plaques per Plate
Dilution
Plate 1 Plate 2 Plate 3
10-6 TNTC TNTC TNTC
10-7 526 530 565
10-8 75 55 60
Volume plated 0.1 ml

Compute the plaque-forming unit (pfu) per ml of the original phage preparation. (5 pts)
10. Can you cry under water?

"Everybody is a genius.

But if you judge a fish by its

ability to climb a tree,
it will live its whole life
believing that it is stupid."

~ Albert Einstein ~