at a @ a as Qs ar Qs Qo ‘Section J: Biotechnology Q.1=Q. 10 carry one mer’ each. (Celis ofmeristemoid ae best deseribed (8) dediferntated ano diving (4) diferemined und rn cviding (Uy dedierented and non dividing (Chasiferenines sd eviding trait process ean nat be use foe 1A) fractonation of proeins (By dest (Co harvesting of ells (Dyssleve removal of solvents “The uinber of replicons in atypical mammalian cells cayan2m ——(8)400 ceyteos20— (0) so000-160000 hat rode wil est rom compe ols fob dato? ‘ap Sucre oly (0) Ft oly (incon rcs only {0} Giese only ‘Aeration in bioretors provided by (A) impelier (bales (©) srarger {Dpall of te above “The transplsiomie plents bear no ri for gore transfer dhrovgh palens us (A) the potens degenerate before fertigation (B) the tanstonmed mitochondrial DNA is lost daring pollen maturation (© the transformed choropist DNA is lost dung pollen maturation {D)the transformed genomic DNA ace ishenied mstermally ‘The mability of DNA in aparse gel cletropharesis is solely based on is (8) c9eformation (ayenaige 1D) none of the above (size ‘Which of the follawine Nuorescen probes is wsed 0 monitor the progress of spification in Realtime PCR? (A)SYBR green (B) Rhodamine #—(C)FITC (©) Cyan blue Expreasion of which ofthe fllowing eparter genes does nt require adiiion of specific subsrae for detection? (4) Lacitesae (8) Glucyronidase (CUM Giocosidase (x) Groen Nuarescent pectinQuo an Qua aut 4s Cbscron Biveuye uty chromatography can be wed fr affinity punieston of (A) NADPIE dchydropenase +B) ghucovmylase (Chsubiiisin (D) corpse 0.11.26 carry twosnarks each A linear DNA |rugment i [00% labeled at we end and ngs 3 ssicon ses for FeeRI. Ais partly digested by EcoRI se tat all possible fragments ave produced how many of these fragments wil be lable and how many will not be Iseled? (A) labeled 6 unsneit (8)4 labeled: wolabcled (C)3 leetes 5 wnlabeled {D)3 labeled: 3 unlabeled ‘Match the fowing products with hie staring subsrtes 2)Soke 1) appl juice Deider 2) grape juice wine 3ytaney lager ane (Aland. bt coddot Bash bodes. dot! (C)a2.b8, ests dood (D) 2-98, bod,c92, dav) eniry the following anbioics wit their moses of eon, Antibiot ‘Mode of ation F 2) Arpicilin 4) inhibition of prvein synthesis by Teuracyeline + 2)inhibition of el wall sytheis 6) Nystatin 3) darmage to cytoplasmic membrane €) Amivamycin Dadamage to DNA svete (A) a,b, nh dead (8x92, bl c3. doe (C)e1. baz codons (D)93, 492d) Ina bioreactor bates are incorporated to (A) prevent vortex and improve veration efficiency (8) maintain uniform suspension of eels (C) minionze the size of air bubble for greater aeration {D) oaintsin uniform auitent mediven Somatic embry fiom coytedon expla would develop in the following seqeestia stages, (ycoiyledonsry+ neat - slobular—+ torpedo (B) globular romped» hear —» cotyledonary (©) elobulsr—+ hear orpedo — cotyledonary Wy coryledonsry.» globulie-» heart — torpedo6 Though the night border (RB) and lel border (LB) ot T-DNA ave identical, he DNA cranes specie ar Ie DNA el of the RB (he T-DNA) ater than or the DNA tof the LB because ‘ La)the sequence conen\ at the RB defines the diese of transfer (B) the sequence content a tbe LB defines the direction of transfer (C)ihe nuclear location saquence (NLS) of VirD? protein dives {D) the endonuclease atinty of Vic? protein allows nicking at RB. escined Tatra DDeiemine the correctness or otherwise ofthe following Assertion [a] ind Reason |e] “Asserlon: An anigen ecognized by one immunogllin subtype is rot recognized by any over aby pe Reason : Immuno obulin abiypes differ from each ober beth in tie variable and in {be constant regi er (4) Both {a} a [sre une and {este cores ean for] (8) Bor [a] and fe are ra bot 1 snot the coret reason fo) (C) Bs [a wd) are Fe () (ais irae but is tase (8 Weskical sized RNA anserpt is detected by Norther blot analysis of UOP slucurenosyl transferase obuained from human liver and kidney, Microaray analysis Of the same samples shows equal spot intensity, whereas Wester boc deiecls a ‘SEDa strong band in ive, bu avery fant band in kidey of same size. The ~ regulation of UDP glucuronosy fnsferas is (A) transenptionsly contelles (©) postransciptionally controled (C)raasiaionaly contolled (©) pos-aslationally conolled 219 Match ine tems on the left column with thve onthe right lett ‘Richt P Beogeamimed cell deaihat sc of infection 1 TMV coat proven 2 Hormone upregulated ding flooding tress 2. EPSP synthase Tages torherticide elyphossie 3. Hypicsensitive response S Pathogen derived resitmce 4. Bunyese (AVP, Q2.R4,83 (2) P3,Q4, 82,541 PL Qarr es? (D/P. QAR SI 2.20 Using the Hil equation foran enzyme [S}a= ( Ye Kal Van Ya! and the plot of logue (val Vea Ye) 5 Fog [SJ one can find out (PY Yau from the intercept on the orinite (Q) Ke from the iescep! on the ordinate 195) "a from the slope (3) Ke fom the intercept othe abscissa Pg QR RS ips0.21 Expression in poor amount and in inactive form pf eDNA of a eukaryotic pretin in Excherichia cok using is expression ‘etx ix due 10 {P) the absence of capping mechanism of mRNA (Q) codon bias (R) sbscnce of polyadenylation (5) stence 0° proper glycosylation rg AR cas Ors ‘Common Data Questions ‘Common Da‘a for Questions 22,23, 24: A recombinant SV40 vius delivers e-nye cDNA, which has a unique Sef I site, nto moscle cells Souther anolysis of SalI digested totl genomic DNA of the musele eels using eye DNA probe generates a smear Q.22 The DNA smear oitines on Southern blot is due t0 (A) heed to head conestamer of viral DNA. (8) head to til conestamer of viral DITA (C) ail total conestamer af vical DNA (D) ramom integration of viel DNA 23. Wester blot analysis of -mye expression of toch transformed cells ast for (A) tansienly () upto five generations {C) upto 10 generations {(D) more than 100 generations Q.24 Which ofthe following types of cancer willbe observed in such transformed els? (A) Adenoma (B)Melanoma_——_(C) Sarcoma (D) Hepatoma Common Data for Questions 25,26: [Normal primary haptoeytes can be aricielly immortalized, Certain spontaneous ratans of immonaiced hepatocyes are eerltve to inizing radiation. Q25 Which of te following genes ace ievolved in Immortlization of primary epsoeytes? (A) Telomerase and Cyclia D (B) NFB and Thymidine kinase (©) Cyclin Dand mye (0) Telomerase and Ras 26 What would happen tothe mutant cells by ionizing radiation? (A) Aporvosie. (8) Necross (OCA growth sees (D)Celt proliferationLinked Anssrer Questions: (Q27a fo Q280 carey ww minchs enc ‘siatenent for Linked Answer Questions 270 & 27) dh ligt of competent E.coli ells were used for deiemanaton of cell dens by pa eeincinethod and another aliquot was used fo transfomation by plasmid DNA. (Q27% 6 colcell cule Lm) was eluted 11009000 and 200 ofthis was Used or ping, Alter 12h incubation ofthe plat, the number clony forming units (CFU) {638 150, Whos the etl CPU per ml in th vigina shore? dyson (Ayr sxio (8) L>x10" (Cp 1 Sx Q2% tiated plasmia BNA (Sng was sed fo wensformation of 100p) competent £ coli cells to whieh 90! of SOC medium was added An aqua of SO was plsted ona Selective plate, After evemightineubation, 300 colonies were observed, Csleuste the tficency of transformation and the percentage of transformed sper mil of parent cele (5) 60:20 colonies per ws of plasmid DNA. 001% @) 1.2110 clones per ug of plasmid DNA, 0.02% {) 1210 colonies per ng of plasmid DNA. 0.008% (0) 60.to* colonies per wg of plasmid DNA, 0.1% Satement for Liked Answer Questions 28a 280. HMGC9A rectae tat binds HMGCOA. is he mar rae limiting step nthe holestel Sonate pathvay, Several inhibitors ef this eye are used as poten divas. The asuy ofa eneyne based on ibeling the eveyne vith adiabled FMGCOA and counting {cp te abled enayme subsate complex inthe presence est andin the absence (Corr of the inhibitor. A lank ise up that comains mo enry™= 238 The percent inhibition for his enzyme is eaflated from the equation (4) {ep (control) ~epm (ext) [epm onteal)~ eps nk} x 100, £8) {fepm (sonra) ~epm (est) {cpm (sak) —epr como} x 100 AC) {fepm (est)~epm (eomnal) [cpm contol) ~epm bisnk Ex 100, (©) {Lepm teonrel) —epm ¢otank)}fepm tess) ep (como) x 100 0285 An inhibitor is considered active if it enuses more thn 65% inion. The cpm ‘ules eexpectively of conte), test a blank samples for inhibitors WX, Yand are pibitors i ctv (A) X ~ $000, 4000 and 100 (iy W = 7000, L400 and 235 KC) ¥ 7500, $000 snd 90 (0)Z~7200, 2900 and 200,