Professional Documents
Culture Documents
focus on Cryptosporidiosis
2
Calf scours
And neonatal diarrhea affecting
small ruminants...
How much is calf scours costing?
Loss of a calf =
price of a heifer to replace + input costs
per died calf : semen and breeding costs,
feeding the dam while she carried the calf
to term and labor through gestation and
calving, resources invested in the calf itself
(labor, vet)
And, long-term financial impact of
scours if the heifers recover :
Increased age at first calving (>30 months)
Reduced ability to meet genetic potential
Increased chance of being culled from herd
NCD is a multifactorial disease
- an adverse environment
- poor host immunity
- challenge from infectious agents
6
Aetiology
2 possible sources :
Faulty feeding
Infectious causes
7
Various studies
detecting frequency
of bovine enteric
pathogens
8
France - Naciri et al., Vet. Parasitol., 1999, 85,
245-257
Detection of
E. coli K99, rotavirus,coronavirus,
cryptosporidium among healthy
(n=14) and diarrheic calves (n=46)
Results :
Diarrheic calves:
43 % Crypto +
46 % Rota +
11
Sweden - Bjorkman et al., Acta Vet. Scand., 2003,
44, 145-152
Healthy 3% 21% 8% 0 2% 0
N= 124
12
a.
Italy Vicenza - Regional Section of the
Venezie Animal Health Testing Institute
between 2005 and 2007.
Enteropathogens isolated in subjects
with neonatal enteritis
13
Italy, Vicenza, 2012
Dairy farm, 101 calves
Coronavirus 3 1 4 10
E Coli F5 1 0 1 2,4
Cryptosporidium 24 7 31 76
15
USA, 165 cattle farms - 2012
E.Coli
Bacteria
Rotavirus Viruses
Coronavirus
Parasites
Cryptosporidium
Diagnosis ?
Clinical diagnosis:
Key elements :
Age
Faeces
Symptoms
18
R
Rotavirus
E.Coli
E.Coli
ETEC
Samonella
Coronavirus Coccidia
Cryptosporidium
Enterotoxemia
Revue Md.Vt.,2009,160,8-9,404-409
The clinical diagnosis
should be based
on the clinical picture including
- the age of the affected animals
- and associated clinical signs
collated through a thorough physical
examination
together with epidemiological data inclu-
ding
- the season
- herd history.
In most situations, the clinical diagnosis
requires confirmation by on-farm or
laboratory tests. 21
Is a precise diagnosis
indispensable?
22
Rapid test in farm
23
Rapid test in farm
METHOD
Immunochromatographic strip test
ANALYSIS
Detection of antigens specific to:
Coronavirus
Rotavirus
Cryptosporidium parvum
E. coli K99(F5)
SAMPLE : Faeces
Focus on colibacillosis
25
Escherichia coli
26
E coli is the most important bacterial
cause of diarrhea in calves during the first
week of life
distinct types of diarrheal disease are
produced by different strains of this
organism
may be differentiated by serotyping
based on antigenic differences in the O
antigen of the LPS, in the flagellar or H
antigens, and the fimbrial or F antigens.
27
Enterotoxigenic E coli
(ETEC) which has two virulence
factors (fimbrial adhesins ): K99
(F5) and F41
28
Escherichia coli pathotypes
Associated with calves diseases
29
Enterotoxigenic E coli (ETEC)
ETEC:
Fimbrial antigens (F5 or F41 or both)enable
them to attach to and colonize the villi of
the small intestine of neonatal calves in the
first days of life these Ag= focus of
immunologic protection
Elaborate also a thermostable non antigenic
enterotoxin(Sta)that influences intestinal
ion and fluid secretion.
30
Pathogenic bacteria contaminating the environment are ingested by
susceptible animals and enter the intestinal tract (1). These bacteria possess
fimbrial adhesins which mediate adherence to specific receptors on the
intestinal epithelial cells (2). Resulting bacterial colonization is found mostly on
the jejunal and or ileal mucosa. The adherent bacteria produce enterotoxins
which stimulate water and electrolyte loss into the intestinal lumen (3), leading
to dehydration and possibly death, and a decreased weight gain in surviving
animals (4). (Source :Ecl, Univ Montreal) 31
EPEC (Enteropathogenic Escherichia coli )
32
Pathogenic bacteria contaminating the environment are ingested by susceptible
animals and enter the intestinal tract (1). Focal to extensive bacterial colonization of
small and large intestine is observed (2). Bacteria produce their own specific receptor
which is injected into the host epithelial cell via a syringe-like bacterial apparatus. A
bacterial adhesin then mediates a very intimate attachment of the bacteria to the cell
receptors and bacterial signals stimulate effacement of the microvilli, or brush border,
and reorganization of the cell cytoskeleton (3). The adherent bacteria also stimulate
epithelial cell degeneration, and infiltration of PMN's in lamina propria. These cell
changes may lead to the appearance of diarrhea (4). (Source :Ecl, Univ Montreal) 33
Zoonotic risk ?
35
What is Cryptosporidiosis?
Oceania
o New Zealand : 21,8%
South America
o Brazil: up to 47%
o Venezuela : 29,3%
North America
o Canada: 15 to 100 %
o USA: 35 % 41%
Asia
o India: 40%
o Japan: 93%
o Korea: up to 100%
Africa
o Zambia: 42,8%
In Europe
LeFay et al. (2000)
Finland :76%
Netherlands: 55%
Spain: 52% - up to 58,5% (J. A. Castro-Hermida, 2006)
Ireland: 44,4%
Italy :40%
France: 43.4%
Germany :40-44%
Hungary:27%
Great Britain : 23 to 32.9%.
Denmark : 17% (Santin (2004) : from 86 to 84% , depending
on the age)
Portugal : 25,4% (Santin (2004))
Epidemiology
Significant morbidity
Mortality : 10 50% (>>>>small ruminants)
Immunity:resistant to subsequent infection after the
initial episode.
Epidemiology
Resistance :
viability of the oocysts from 3 months
(at15C) to more than 1 year (at 4C) =>
High prevalence
Disinfection difficult
Pathophysiology
Tropism: Gut
46
Pathophysiology
Tropism: Gut
Location : epithelial cells:
47
48
Pathophysiology
Tropism: Gut
hard to treat !
49
Pathophysiology
Tropism: Gut
Location : epithelial cells:
Intracellular, extracytoplasmic
=> hard to treat !
Villous atrophy and epithelial
cells loss
leads to a
malabsorptive
diarrhea
50
Upon necropsy, what will you see in the
intestine?
Thick mucoid content
51
Life cycle of cryptosporidium
Key points
Total duration
: only 4 days
All the stages
of
development
inside the host
2 ways of
reinfection
Villus
4 days
53
4.GAMETOGONY
Ways of
autoinfection
3.MEROGONY
2. SPOROGONY
1
54
On the field.
Signs of Crypto?
Age of calves: 2de week after birth
History of the farm (Hygiene, livestock
buildings)
Calving period ( higher infection pressure in
the second half)
Recurrent diarrheal episodes
High morbidity (anorexia - dehydration ) Low
mortality
Symptoms: mucoid diarrhea
Curative?
Treatment of calves diarrhea?
Several factors involved therapy must
often be started before an etiologic
diagnosis has been established.
Fluid and electrolyte therapy is most
important
Majority of cases of bacteremia and
septicemia in neonatal calves are
associated with E coli =>the chosen
antibiotic should be effective against
gram-negative bacteria
Provide analgesia (NSAIDs)seems to help
a faster recovery 61
Reduced and responsible
use of Antibiotic
Prescribed by a veterinarian
62
Curative, metaphylactic use
63
Treatment ?
66
Some tested molecules .
67
+ alternative therapies ?
Indications:Treatment of gastro-intestinal
infections caused by Escherichia coli susceptible to
paromomycin
71
Parofor
72
Aminoglycosides: several groups
based activity
Narrow spectrum
Streptomycin
Broad spectrum
Apramycin
Extended spectrum
Paromomycin (including antiprotozoic activity)
73
Parofor
Aminoglycoside with Expanded
spectrum
Bacterial
&
Antiprotozoal spectrum:
o Cryptosporidium spp
o Giardia
(Alejandro Grinberg, 2002; E.H. Johnson, 2000; Viu et al.; 2000; Grinberg et
al., 2002)
o Histomoniasis (poultry species)
o Leishmania (companion animals, human) 74
ACTIVITY : Bactericidal
amoxycilline
Tylosin, tilmicosin
colistine paromomycin
enrofloxacine
75
Mode of action: double
76
Pharmacodynamics
Concentration
MIC
Time
Daily dose 1x/ day
Pharmacokinetics
78
THANK YOU FOR YOUR ATTENTION
79