Houston

Targeted Proteomics
Targeted Biomarker Screening and Valida7on Services
Targeted Proteomic Screening of 2300 Proteins
SOMAscan Aptamer Screen
SOMAscan allows for the simultaneous detec%on of 1300 human proteins using
SOMAmer (Slow O-rate Modied Aptamer) protein-binding reagents.
Methods: (a) SOMAmers aYached to beads
bind to proteins in the sample mix. (b)
Proteins that are bound to their specic
SOMAmer reagents are bio%nylated, and (c)
(b)
(a)
the SOMAmer-protein complexes are
released by a photocleavable linker. (d) The
bio%nylated proteins are bound to a second
streptavidin bead, (e) where the bound
SOMAmer reagents are removed from their
protein targets. (f) Released SOMAmer
(e)
(d)
reagents are collected, denatured, and
quan%ed using a custom DNA microarray. Sample Volume: 75-150l
Detec7on Range: fM to M

(c)

(f)

RayBiotech An7body Array

The RayBiotech L-series an%body array uses the pre-labeling of proteins in the
sample mix to simultaneously detect 1000 human proteins on an an%body
immobilized glass slide array.
(a)

(b)

(c)

Methods: (a) The sample mix is dialyzed

and all proteins are bio%nylated. APer
dialyzing again, (b) the bio%nylated proteins
are added to an an%body array. (c)
Fluorescent labeled streptavidin allows for
the quan%ta%on of bound proteins using a
microarray scanner.

Sample Volume: 50-400l

Detec7on Range: pM to nM
houstontargetedproteomics@gmail.com hYp://mohanlab.bme.uh.edu/htp/

Houston Targeted Proteomics

Targeted Biomarker Screening and Valida7on Services
Follow-Up Valida%on
ELISA
The gold standard for protein quan%ta%on, ELISA, is a plate based detec%on
system that tradi%onally uses an%bodies for single target detec%on.
Methods: In a sandwich ELISA format (a),
target protein from the sample mix is
captured in a plate well using capture
an%body, while in a compe%%ve ELISA (b),
the target protein from the sample mix and
labeled target protein compete for binding
to the capture an%body. An enzymelabeled secondary an%body allows for the
quan%ta%ve detec%on of the colorimetric
(a)
signal. Target protein concentra%ons can
be extrapolated using a standard curve.
Sample Volume: 5-100l

Detec7on Range: pM to nM


Meso Scale

(b)

Meso Scale is a mul%plexed plate based assay that allows for the simultaneous,
quan%ta%ve detec%on of up to 10 proteins from a single, small volume sample.
(a)

(b)

(c)

(d)

Methods: (a) Capture an%bodies are immobilized

onto working electrodes using specialized linkers.
(b) Sample mix is added and target proteins bind
to their corresponding an%body. (c) Sulfo-tag
labeled detec%on an%body is added. (d) Each
working electrode is ac%vated to individually
quan%fy the electrochemiluminescent signal of the
target. Target protein concentra%ons can be
extrapolated using a standard curve.
Sample Volume: 5-25l
Detec7on Range: pM to M

houstontargetedproteomics@gmail.com hYp://mohanlab.bme.uh.edu/htp/