Purpose Scientific research requires a logical progression of steps that clearly define and focus the work being done. These steps are guided by a specific question or a problem. Research is rarely linear. Instead, scientists must be flexible, creative, and willing to change strategies. Persistence is essential as there are many more failures than successes in research. These failures often result in new strategies and new questions re-focusing research. When designing an experimental procedure there are guidelines that will help you keep a logical progression of steps. Experiments have controls which allow for comparison and help prevent incorrect conclusions being made due to inconsistent conditions impacting the data. The following experimental design is a guideline to be used when creating experiments in the PLTW Biomedical Science courses. It is not a strict scientific method protocol. It is a guide to help you create logical, thoughtful and clearly defined procedures for investigative work. You are expected to follow these guidelines when designing experiments. Use your Lab Journal for recording all experimental design work. When recording your work write the number and title of each section as shown below. Following each design step is an explanation (shown in italics), of the format that is to be used in your lab journal for that specific step. The evaluation rubric is based on following the steps of design and using the correct format to record the information in your journal.
Steps of Experimental Design
1. Identify the Problem or Question What effect a persons heart rate? 2. Predict a solution to the problem or an answer to the question. Variations in breath will cause an increase in a persons heart rate. 3. Design the experiment to be used to test your hypothesis. 1. Plug in the LabQuest Mini into your computer 2. Plug the Bluetooth heart rate monitor into the the Lab quest mini 3. Open Logger Pro on computer 4. Open file from Human Physiology with Vernier then open 03 Heart Rate Vital Signe file 5. Hold hands on heart rate monitor and have the Bluetooth receivers arrow facing upwards 2013 Project Lead The Way, Inc. Principles of Biomedical Science Biomedical Sciences Experimental Design Page 1
Carter and Leila
6. Then click the collect button 7. Let it collect data for 60 sec while sitting and breathing normal 8. Repeat this twice 9. Collect average 10. Rest for a minute 11. Let it collect data for 60 sec while sitting and not breathing 12. Repeat this twice 13. Collect average 14. Rest for a minute 15. Let it collect data for 60 sec while sitting and breathing rapidly 16. Repeat this twice 17. Collect average 18. Rest for a minute 4. Carry out the experiment. Effects on Heart Rate Breathing
Carter
Leila
Average
Normal
70
96
83
None
100
105
102.5
Rapid
170
89
129
5. Analyze the data and observations.
Effects on Heart Rate
200 150
BPM 100 50 0 Normal
None
Rapidly
Rate of Breathing carter
Leila
Average
6. State the conclusion.
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The hypothesis was correct in that variations in breathing will increase a persons heart rate. 7. Summary Paragraph In this experiment, the hypothesis was proven to be correct by the results in the data table above. The reason why this happened is because a persons breathing is closely related to a persons heart rate. Because in order for the heart to function properly it needs and oxygen supply, and if this is affected so is the effectiveness of the heart. This is true because any excertion on the heart will increase its rate. This experiment could be improved by doing more trials and by having only one persons heart rate being taken.
2013 Project Lead The Way, Inc.
Principles of Biomedical Science Biomedical Sciences Experimental Design Page 3