Professional Documents
Culture Documents
Pavon),
Oca (Oxalis tuberosa Molina) and
ulluco (Ullucus tuberosus Caldas)
David Campos,1 Giuliana Noratto,2 Rosana Chirinos,1 Carlos Arbizu,3 Willian Roca3
and Luis Cisneros-Zevallos2
1 Instituto
Abstract: Four species of edible tubers endemic to and domesticated in the Andes, native potato (Solanum sp.),
INTRODUCTION
There is a continuous search for new plant compounds with antioxidant potential. Interest in natural antioxidants has increased considerably in recent
years.1 4 Antioxidants, which can neutralize free radicals, may be of central importance in the prevention
of cancer and cardio- and cerebrovascular diseases.5 9
Many natural antioxidants exhibit a wide range of
biological effects, including antibacterial, antiviral,
anti-inflammatory, antiallergic, antithrombotic and
vasodilatory activity.10 The Andean region is well
known for its great genetic diversity in different types of
crops, which unfortunately have been poorly studied.
Andean crops show variability in colors, forms, sizes,
primary nutrient constituents and bioactive secondary
metabolites.
People from the Andean region have utilized native
potato (Solanum sp.), mashua (Tropaeolum tuberosum
Correspondence to: Luis Cisneros-Zevallos, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843-2133, USA
E-mail: lcisnero@taexgw.tamu.edu
(Received 17 February 2005; revised version received 26 September 2005; accepted 20 February 2006)
Published online 15 June 2006; DOI: 10.1002/jsfa.2529
D Campos et al.
705841
704463
707132
705263
703985
705024
703286
706819
0
704353
0
705468
1000
702961
703279
2000
703825
703844
3000
5000
704481
HAC
4000
11500
12
ACY
HAC
9500
-500
M6COL2C
ARB-5576
ARB-5241
DP-02-24
1500
ARV-5366
DP-02-15
3500
AGM-5109
AVM-5562
5500
DP-02-07
DP-02-23
7500
DP-02-03
TPH
10
6500
TPH
ACY
5500
HAC
4500
2.5
3500
2
2500
1.5
1500
3.5
500
0.5
-500
O-018-83
AAQ-5477
MU-31
AMM-5223
O-116-84
PICA-HI-92 OC
COC-537
DP-01-46
AJA-5252
ATT-5466
AJA-5245
O-266-85
0
AJA-5270
ACY
ARB-5054
TPH
Statistical analysis
SPSS for Windows version 11.0 was used for
statistical analysis. Pearson correlation at the level
of = 0.01 and analysis of variance (ANOVA) with
mean comparisons using Tukeys multiple range test
at = 0.05 were performed. Results are expressed as
average standard deviation (SD).
6000
yellow mashua genotypes. The TPH content of ARB5241 was comparable to that of strawberries, used as
a reference (3.35 mg g1 ). For oca tubers, the TPH
ranged from 0.71 to 1.32 mg g1 (Fig. 3). Genotypes
O-018-83, MU-31 and AAQ-5477 showed the
highest TPH values with 1.32, 1.31 and 1.26 mg g1 ,
1483
D Campos et al.
2200
TPH
1.4
TBX
HAC
1700
1.2
1
1200
0.8
700
0.6
0.4
200
1.6
0.2
-300
U-108-84
U-034-83
AJA-5275
DPA-03-23
PRODEKON-16
MH-296
DPA-03-43
AQP-5454
CLC-004
U-09-0122
CLC-053
ARV-5337
PRODEKON-14
U-09-0124
AMM-5142
Genotype
707 132
705 024
705 841
704 463
703 286
703 825
705 263
703 985
704 353
702 961
705 468
706 819
703 279
704 481
703 844
a
b
Common
name
Yana Shiri
Pachacona
Unknown
Gogon Magash
Yana Pituwayaka
China Runtush
Chaucha
Runtu
Puna
Garhuash Pashon
Huasa Amarilla
Tablera Jubalena
Seda Monilla
Amarilla
Anil Papa
Species
S. curtilobum
S. tuberosum subsp. tuberosum
S. tuberosum subsp andigena
S. tuberosum subsp andigena
S. stenotomum
S. goniocalyx
S. phureja
S. goniocalyx
S. tuberosum subsp andigena
S. goniocalyx
S. goniocalyx
S. tuberosum subspp. andigena
S. goniocalyx
S. goniocalyx
S. stenotomum
Color of
flesh/skina
Moisture
(%)b
TCT
(g g1 )b
LAC
(g TE g1 )b
P/P
P/P
R/R
P/P
P/Y
Y/Y
Y/Y
Y/Y
Y/Y
Y/Y
Y/Y
Y/Y
Y/Y
Y/Y
Y/Y
78 3.9f
76 1.6e,f
75 1.0d,e,f
75 1.2d,e,f
74 0.7c,d,e,f
72 3.9c,d,e,f
72 4.9c,d,e,f
70 0.7c,d,e
70 2.9c,d,e
70 0.5c,d,e
69 3.3c,d,e
69 1.7c,d,e
69 3.0c,d,e
68 1.5c,d
66 2.8c
ND
4 0.1d,e,f
ND
ND
ND
4 1.3d,e,f
4 1d,e,f
4 1e,f
2 0c,d
2 1c,d,e
5 1f
4 0d,e,f
3 1c,d,e,f
3 0c,d,e,f
4 1d,e,f
321 15e,f
211 7c,d,e
152 35c,d
131 26c,d
357 30f
133 53c,d
239 102c,d,e,f
155 82c,d
199 76c,d,e
170 40c,d
249 30c,d,e,f
115 28c
125 19.21c,d
265 28d,e,f
361 13f
Genotype
Color of
flesh/
skina
Moisture
(%)b
TCT
(g g1 )b
LAC
(g TE g1 )b
AVM-5562
DP-02-07
DP-02-15
M6COL2C
DP-02-23
AGM-5109
DP-02-03
DP-02-24
ARB-5576
ARB-5241
ARV-5366
Y/Y
Y/Y
Y/Y
Y/Y
Y/Y
P/Y
Y/Y
P/P
Y/Y
P/Y
Y-P/P
92 1.6c
90 1.0c,d
90 1.9c,d,e
90 1.6c,d,e
90 1.6c,d,e
89 1.2c,d,e
87 2.8c,d
87 1.3c,d
87 1.4c,d
85 1.7c
86 0.6c
14 3d,e
21 3d,e
17 1d,e
23 6e
13 6d,e
18 0d,e
21 4d,e
ND
25 9e
10 1c,d
1 0c
217 37.7e,f
221 13.0e,f
186 9.6d,e
279 15.0f
93 22.85c
128 56.1c,d
224 24.7e,f
ND
218 12.8e,f
202 19.3d,e,f
225 44e,f
Genotype
PICA-HI92OC
O-116-84
O-266-85
ARB-5054
ATT-5466
COC-537
MU-31
AMM-5223
AJA-5245
AAQ-5477
AJA-5270
O-018-83
DP-01-46
AJA-5252
Color of
flesh/
skina
Moisture
(%)b
TCT
(g g1 )b
LAC
(g TE g1 )b
Y-R/P
82 1.4c,d,e
8 0c,d
165 23c,d,e
Y-P/P
Y/Y
Y/Y
Y-R/P
Y-P/P
Y-P/P
Y-R/P
Y/Y
Y-P/P
Y/Y
P-Y/P
Y-P/P
Y/Y
80 1.7c,d
82 2.0c,d,e
84 1.5c,d,e
84 0.7c,d,e
85 2.6e
85 0.8e
80 1.0c
84 0.2c,d,e
82 0.8c,d,e
84 0.8c,d,e
85 1.3d,e
81 1.3c,d,e
84 2.4d,e
7 1c,d
13 2d,e
14 1d,e
5 1c,d
2 1c
3 1c
3 1c
25 4f
11 9c,d,e
17 2e,f
3 1c
3 1c
10 2c,d,e
126 44c,d
164 39c,d,e
101 4c,d
219 33d,e,f
268 41e,f
76 24c
69 58c
127 34c,d
320 59f
90 25c,d
181 30c,d,e
135 79c,d,e
107 72c,d
704 463 and 707 132 showed the highest HAC values
with 3780, 3369 and 2473 g TE g1 , respectively.
Potatoes are known to contain water-soluble phenolic
antioxidants that act as radical scavengers such as
quercetin and chlorogenic acid.31 We found that
increasing TPH in tubers showed a trend of increasing
HAC values (Fig. 1), despite the low correlation
between HAC and TPH (r 2 = 0.45, P = 0.00). A
high correlation between HAC and TPH for different
genotypes in a certain type of crop would only be
possible if the genotypes have phenolic compounds
with similar antioxidant properties (e.g. similar protondonor properties). Since the correlation obtained
is low, we hypothesize that the studied native
potatoes had different phenolic profiles (qualitative,
1485
D Campos et al.
Figure 5. Andean tubers with high antioxidant capacity: (A) native potato 707 132; (B) mashua ARB-5241; (C) oca O-018-83; (D) ulluco U-108-84.
CONCLUSIONS
This survey of Andean tuber germplasm for antioxidant compounds confirms that each type of crop
studied can be considered as an excellent source of
dietary phytochemicals (Fig. 5). The phytochemicals
responsible for the antioxidant capacity are related
to the phenolics and carotenoids present in each
tuber. The antioxidant values of these tuber crops
are higher than or comparable to known sources of
natural antioxidants, such as blueberries, indicating
that these crops have the potential to be considered
as important novel sources of nutraceuticals. These
Andean tuber crops hold great promise for producing
new and unique healthy, functional products for the
benefit of producers and consumers.
ACKNOWLEDGEMENTS
Carla Rios, Daniel Segura and Katherine Alejandro
are thanked for technical assistance.
REFERENCES
1 Loliger
J, The use of antioxidants in foods, in Free Radicals and
Food Additives, ed. by Arouma OI and Halliwell B. Taylor and
Francis, London, pp. 121150 (1991).
2 Liu RH, Health benefits of fruit and vegetables are from additive
and synergistic combinations of phytochemicals. Am J Clin
Nutr 78:517S520S (2003).
3 Boyer JB and Liu RH, Apple phytochemicals and their health
benefits. Nutr J 3:5 (2004). Available: http://www.nutritionj.
com/content/3/l/5 [15 April 2005].
4 Arts ICW and Hollman PCH, Polyphenols and disease risk in
epidemiologic studies. Am J Clin Nutr 81:317S325S (2005).
5 Ames BM, Shigena MK and Hagen TM, Oxidants, antioxidants
and the degenerative diseases of aging. Proc Natl Acad Sci USA
90:79157922 (1993).
6 Gey KF, The antioxidant hypothesis of cardiovascular disease: epidemiology and mechanisms. Biochem Soc Trans
18:10411045 (1990).
7 Steinberg D, Parthasarathy S, Carew TE, Khoo JC and Witztum JL, Beyond cholesterol: modification of low-density
lipoprotein that increased its atherogenicity. N Engl J Med
320:915924 (1989).
8 Steinberg D, Antioxidants and atherosclerosis: a current
assessment. Circulation 84:14201425 (1991).
9 Liu RH, Potential synergy of phytochemicals in cancer
prevention: mechanism of action. J Nutr 134:3479S3485S
(2004).
10 Cook NC and Samman S, Flavonoids chemistry, metabolism,
cardioprotective effects and dietary sources. Nutr Biochem
7:6676 (1996).
11 King SR and Gershoff SN, Nutritional evaluation of three
underexploited Andean tubers: Oxalis tuberosa (Oxalidaceae),
Ullucus tuberosus (Basellaceae) and Tropaeolum tuberosum
(Tropaeolaceae). Econ Bot 41:503511 (1987).
12 Gross R, Koch F, Malaga I, de Miranda AF, Schoeneberger H
and Trugo LC, Chemical composition and protein quality
of some local Andean food sources. Food Chem 34:2534
(1989).
13 Swain T and Hillis W, The phenolic constituents of Prinus
domestica. I. The Quantitative analysis of phenolic constituents. J Sci Food Agric 10:6368 (1959).
14 Fuleki T and Francis F, Quantitative methods for anthocyanins.
1. Extraction and determination of total anthocyanin in
cranberries. J Food Sci 33:7277 (1968).
15 Abdel-Aal ES, A rapid method for quantifying total anthocyanins in blue aleurone and purple pericarp wheats. Cereal
Chem 76:350354 (1999).
1487
D Campos et al.
16 Talcott S and Howard R, Phenolic autooxidation is responsible
for color degradation in processed carrot puree. J Agric Food
Chem 47:21092115 (1999).
17 Cai YZ and Corke H, Production and properties of spray-dried
Amaranthus betacyanin pigments. J Food Sci. 65:124852
(2000).
18 Arnao M, Cano A and Acosta M, The hydrophilic and
lipophilic contribution to total antioxidant activity. Food Chem
73:239244 (2001).
19 Awika JM, Rooney LW, Wu X, Prior RL and CisnerosZevallos L, Screening methods to measure antioxidant activity
of sorghum (Sorghum bicolor) and Sorghum products. J Agric
Food Chem 51:66576662 (2003).
20 Reyes LF and Cisneros-Zevallos L, Wounding stress increases
the phenolic content and antioxidant capacity of purpleflesh potatoes (Solanum tuberosum L.). J Agric Food Chem
51:52965300 (2003).
21 Dini A, Rastrelli L, Saturnino P and Schettino O, Minor
components in food plants Note I. Flavonol glycosides from
Ullucus tuberosus. Boll Soc Ital Biol Sper 67:10531058 (1991).
22 Giusti M and Wrolstad R, Radish anthocyanin extract as a
natural red colorant for maraschino cherries. J Food Sci
61:688694 (1996).
23 Cevallos-Casals BA and Cisneros-Zevallos L, Stoichiometric
and kinetic studies of phenolic antioxidants from andean
purple corn and red-fleshed sweetpotato. J Agric Food Chem
51:33133319 (2003).
24 Lu W, Haynes K, Wiley E and Clevidence B, Carotenoid
content and color in diploid potatoes. J Am Soc Hortic Sci
126:722726 (2001).
25 Iwanzik W, Tevini M and Hilbert R, Carotinoidgehalt undzusammensetzung verschiedener deutscher Kartoffelsorten
die Fleischfarbe der Knolle. Potato
und deren Bedeutung fur
Res 26:149162 (1983).
26 Muller
H, Determination of the carotenoid content in selected
vegetables and fruits by HPLC and photodiode array
detection. Z Lebensm Unters Forsch 204:8894 (1997).
27 Olmedilla B, Granado F, Blanco I, Vaquero M and Cajigal C,
Lutein in patients with cataracts and age-related macular
degeneration: a long-term supplementation study. J Sci Food
Agric 81:904909 (2001).
1488