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INTRODUCTION
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TRANSCRIPTOMICS
Figure 1:
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Figure 1:
Work flow of gene array analysis. Diagrammatic representation of DNA
microarray data analysis and relative limitations under each category of
data analysis during data mining.
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APPLICATIONS OF DNA MICROARRAY IN BIOREMEDIATION
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LIMITATIONS OF DNA MICROARRAYS IN BIOREMEDIATION
The specificity of the extraction method plays a central role and should
vary depending on the site of sampling; as there must be sufficient
discrimination between probes. One can achieve a discrimination level of
a single base pair between probes by imposing a temperature gradient
and recording signal intensities [33]. However, it should be borne in
mind that this method is less sensitive than PCR [31], which might be
crucial in sequence analysis of poor abundance. Also, there is a
promising perspective for microarrays in determining the relative
abundance of a microorganism bearing a specific functional gene in a
complex environment. Over a range of 1100ng of target genomic DNA
concentration Wu et al. [34] observed a linear relationship between
signal intensity and target DNA from pure and mixed culture
communities. However, specificity is a key issue, since one needs to
distinguish the differences in hybridization signals due to population
abundance from those due to sequence divergence.
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PROTEOMICS
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INTERACTOMICS
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CONCLUSION