PHARMACOLOGY LABORATORY

REPORT

TITLE: Determination of the

pharmacokinetics parameters of sodium
salicylate in vivo in rabbits
GROUP MEMBERS: - Eric Kobby
Ankomah(913905006)
-

Annie Jireeh Meli(913905022)

Iqra Awan(913905050)
Tseengun Batbold(913905019)
Kwadwo Sarpong(913905004)

Mentor: LIU QIANG

Tel: 13198997446

Date of experiment: 2016-04-11

Purpose: This experiment is aimed to help the
students to comprehend the regularity of the
variation of time-dependent blood concentration of
sodium salicylate after intravenous injection.
Materials:
1.50 Kg

- One rabbit with a body weight of

-10% sodium salicylate, 6%

trichloroacetic acid (TCA), 0.04% sodium salicylate,
heparin
coated
tubes,
centrifuge
tubes,
chromogenic reaction tubes
- Centrifuge, 721 spectrophotometer,
measuring pipettes, marker pen, cotton wool
Methods:

1. Preparation before experiment

- Take 10 centrifugal tubes and
number them from c0 to c9,to
each tube, add 2Ml of 6% TCA
- Take 8 heparin-coated tubes and
number them h0 to h7

2. Administration and collection of blood sample

-take a rabbit and record its weight
-lacerate the ear edge vein to collect blood from
the rabbit and put into the heparin tubes and shake
for the heparin to mix with the blood
-inject 10% sodium salicylate (SS) at 2Ml/Kg to the
other ear

- The serum samples are collected after drug

administration, at 5,10,15,20,45,60,75 min after
respectively and put into h1-h7
And record the time.
3. Treat the blood sample
- 0.6Ml of blood sample from h0 to h7 tubes+2 Ml
of 6% TCA+0.6 Ml distilled water where put into c0
to c9 tubes
-0.6Ml of 0.04%SS +0.6 Ml distilled water where
added into the c0 to c9 tubes
-the tubes were well shaken and centrifuged at
3000rpm for 15 minutes.
-6Ml of the supernatant was collected from each
tube and the optical density was measured by the
spectrophotometer at 510nm, we got d0 to d9.
4. Calculation of the blood concentration of the
drug

d0 C0: 0.202/0.764X400=105.7g/mL
d1 C1: 0.461/0.764X400=241.3 g/mL
d2 C2: 0.541/0.764X400=283.2 g/mL
d3 C3: 0.648/0.764X400=339.2 g/mL
d4 C4: 0.457/0.764X400=239.2 g/mL
d5 C5: 0.288/0.764X400=150.7 g/mL

TEST
TUBE

TIME 6% BLOO
(min TCA D
s)
(ml
(ml)
)

OTHER
(standar
d
solution
)

OPTICA CONCENTRATI
L
ON
DENSIT
(g/mL)
Y

Standard
tube

0.6ml

0.764 400

Before
Administrat
ion

0.6

0.202 105.7

After
Administrat
ion

0.6

0.461 241.3

10
15
30
45

2
2
2
2

0.6
0.6
0.6
0.6

0.541
0.648
0.457
0.288

283.2
339.2
239.2
150.7

DISCUSSION
The optical density of the standard tube C 5 was the
highest. d0 which was the optical density of the
tube containing the blood before the drug was

administered was lowest because there was no

drug in the blood.
d1 to d5 showed a gradual decline and as C 1 to C5
also decline. This is because concentration could
have started to reduce after 5 mins and
subsequent times due to distribution as well as
elimination.

CONCLUSION
The drug is administered via IV, the concentration
is high, with time, the drug is distributed to the
tissues and starts to go through the process of
elimination. This leads to the decrease in the
concentration as time goes on.