Hot Zone Part 3

By Varun Gunda
(PART III - Due December 9th) Fill out a lab journal detailing at least 4
experiments/procedures from the perspective of different characters. The journal entries must
contain the date, procedural steps, results, a description of lab thats being performed, and
pictures of required equipment and results for each test/procedure that is discussed. (Labs from
parts I and II of the book can be used in the journal)

Lab Entry 1
Thomas Geisbert
Monday, November 27, 1989 (Page 191-195)
After coming back from my hunting trip with my friends during Thanksgiving, I went
directly to the Institute. I am extremely anxious to see the dead monkey cells I had harvested
earlier. The experiment I was performing was to analyze the monkey cells to find visual evidence
that the monkeys were infected with simian fever. I first removed a
diamond knife from the filing cabinet and carried a crumb of infected cells
into the cutting room. Then, I placed the diamond knife into the cutting
machine. I look into the microscope and turn the cutting machine on. The
cutting machine sliced the crumb of cells into small pieces. The slices fell
onto a droplet of water. I took an eyelash and separated the slices, but I
lifted out the damaged slices. Next, I picked up a small metal grid with
tweezers and dipped into the water to pick up a slice, then I put it into a
tiny box. This was placed in the sample holder of my electron microscope for examination. I
looked into the microscope and zoomed in. I was shocked to see a kind of virus that looked like a
filovirus.

It looked like Marburg!

Diamond Knife
http://www.bbraun.ie/cps/rde/xchg/cw-bbraun-en-ie/hs.xsl/products.htm
l?prid=PRID00004575
Electron Microscope
http://www.nano.pitt.edu/node/237

Lab Entry 2
Nancy Jaax
September 26, 1983 (Page 69-87)
I entered the Ebola Suite and removed all of my clothes and put on a sterile surgical scrub
suit. Then, I entered level 2, then 3. I put on some clean white socks and latex gloves. I taped
them on. Then, Lieutenant Colonel Johnson entered wearing a scrub suit. Both of us put on
Chemturion biological suits and plugged in air hoses to our suits
before entering level 4. The purpose of this experiment was to perform
a necropsy and examine the insides of a monkey that had been infected
with Ebola. As soon as we entered the room, the monkeys went crazy
and started hooting. There were 2 control monkeys
used to compare them to the ones infected with
Ebola. We found 2 monkeys that had bled out. I
opened the cage of an Ebola infected monkey that
was unconscious and pinched it in the toe to make
sure it was down.We put the monkey in a hatbox and took it into a separate
room to perform necropsy. I laid the monkey on the table and opened it up
with scissors. I used a scrub and scrubbed all the infected blood out of the
monkey, and Johnson used a rongeur to crack open the skull and took a
sample from the monkey. The results showed that there were a lot of hemorrhages inside the
body. This was when the Lieutenant pointed to the cut in my suit. I became frightened!
Rongeur
http://www.stoeltingco.com/neuroscience/surgical-instruments/mic
rodissecting/bone-rongeurs.html
Chemturion Biological Suit
http://archive.constantcontact.com/fs092/1102938289998/archive/1
103866590242.html

Lab Entry 3
Peter Jahrling
November 28, 1989 (215-217)
After entering my own lab, hot zone AA-4, I tried to solve this monkey problem as fast as
I could. In this lab I was trying to compare the blood sample from the monkey house with other
Ebola strains to match them. These tests included involved making samples glow under
ultraviolet light. I went to the freezers and got out vials of serum from three human victims:
Musoke, Boniface, and Mayinga. Each of these would test for different strains of Ebola. First, I
put droplets of cells from the monkeys and treated them chemicals, then I put them on slides.
These would glow in the presence of the target virus. The room had to dark for this procedure.
Later, I looked into the microscope to compare the viruses. The Musoke did not glow, the
Boniface didnt glow either. However, the mayinga strain glowed which meant that the monkeys
were infected with Ebola Zaire!

Vials
https://www.nudenicotine.com/product/clear-glass-vials/
Microscope
http://www.microscope.com/student-microscopes/middle-high-school
-student-microscopes/omano-om88-1600x-clinical-microscope.html#g
ref

Lab Entry 4
Jerry Jaax
December 4-5, 1989 (Page 317-331)
I left home at 4 oclock to go to the monkey house. The procedure we were going to
follow was to euthanize all the monkeys in the monkey house. I first briefed all of my team
members and grouped them according to the buddy system. Me and my team dressed up in space

suits and went in fed the monkeys biscuits so that they would quiet down. I instructed the others
to fill a double dose of ketamine to euthanize the monkeys. There were a total of four hundred
fifty monkeys. First, we went to each cage and pinned the money down. Then, we injected it
with anesthetic. Lastly, we gave it a double dose of ketamine to euthanize the monkey. We then
dissected the monkey to get samples. After the procedure was complete, we went through a
decon shower of bleach to kill any lingering virus particles. In the end, all the monkeys were
killed!

Chemturion Biological Suit

http://archive.constantcontact.com/fs092/1102938289998/archive/1
103866590242.html
Ketamine and Syringe
http://www.everydayhealth.com/columns/therese-borchard-sanity-br
eak/ketamine-miracle-drug-depression/