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CHAPTER
11
Synovial Fluid
Key Terms
ANTINUCLEAR ANTIBODY
ARTHROCENTESIS
BULGE TEST
CRYSTAL-INDUCED ARTHRITIS
GROUND PEPPER
HYALURONATE
MUCIN
OCHRONOTIC SHARDS
RHEUMATOID ARTHRITIS (RA)
RHEUMATOID FACTOR (RF)
RICE BODIES
ROPES TEST
SEPTIC ARTHRITIS
SYNOVIAL
SYSTEMIC LUPUS ERYTHEMATOSUS
VISCOSITY
Learning Objectives
1.
2.
3.
4.
5.
6.
7.
8.
9.
Define synovial.
Describe the formation and function of synovial fluid.
Explain the collection and handling of synovial fluid.
Describe the appearance of normal and abnormal synovial fluids.
Correlate the appearance of synovial fluid with possible cause.
Interpret laboratory tests on synovial fluid.
Suggest further testing for synovial fluid, based on preliminary results.
List the four classes or categories of joint disease.
Correlate synovial fluid analyses with their representative disease classification.
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oint fluid is called synovial fluid because of its resemblance to egg white. It is a viscous, mucinous substance
that lubricates most joints. Analysis of synovial fluid is
important in the diagnosis of joint disease. Aspiration of
joint fluid is indicated for any patient with a joint effusion
or inflamed joints. Aspiration of asymptomatic joints is
beneficial for patients with gout and pseudogout as these
fluids may still contain crystals.1 Evaluation of physical,
chemical, and microscopic characteristics of synovial fluid
comprise routine analysis. This chapter includes an
overview of the composition and function of synovial fluid,
and laboratory procedures and their interpretations.
Bone
Periosteum
Ligament
Articular
capsule:
Articulating
bone
Synovial (joint)
cavity (contains
synovial fluid)
Fibrous capsule
Synovial
membrane
SPECIMEN COLLECTION
After finding positive results with a bulge test (Fig. 11-3),
the physician will perform an arthrocentesis and aspirate
the effected joint. An appropriate gauge needle is attached
to a syringe and the entry site is cleansed. A two-step
process is employed for arthrocentesis in which the first
puncture is made through the skin followed by a second
thrust into the synovial capsule. Figure 11-4 illustrates
needle placement in arthrocentesis of elbow and knee joints.
After fluid is aspirated and the needle withdrawn from
the joint, the needle is removed and an end cap placed on
the tip of the syringe. The syringe is properly labeled and
sent to the laboratory for testing.1 Some laboratories
Articular
cartilage
Articulating
bone
Ligament
Lateral thigh
Figure 11-3. Bulge test of joint for the detection of synovial effusion.
255
LABORATORY TESTING
Lateral view
B
Figure 11-4. Placement of needle in arthrocentesis of (A) elbow and
(B) knee joints.
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(3 cm) or run out of the syringe and down the side of
the test tube like water.1,4 Low viscosity of synovial
indicates the presence of an inflammatory process.
Figure 11-7 illustrates the performance of the string
test for synovial fluid viscosity.
Clotting. Clotting of synovial fluid can result when fibrinogen is present. Fibrinogen may have entered into
the synovial capsule during damage to the synovial
membrane or as a result of a traumatic tap.3 Clots in
specimens interfere with performance of cell counts.
Depositing part of the specimen into a tube containing heparin may help avoid clotting of synovial fluid.
Mucin clot. The mucin clot test, also known as Ropes test,
is an estimation of the integrity of the hyaluronic
acidprotein complex (mucin). Normal synovial fluid
forms a tight ropy clot upon the addition of acetic acid.
AUTHOR
Brunzel3
4
Strasinger
Table 11-1
CHEMICAL EXAMINATION
VOLUME OF
SYNOVIAL FLUID
One part
Four parts, 2%
One part
Four parts, 2%
Two parts
One part, 3%
Not specified
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that are increased during the acute phase of these disorders contribute to this increased LD level.6
Rheumatoid factor. Rheumatoid factor (RF) is an antibody
to immunoglobulins.4 RF is present in the serum of
most patients with RA, whereas just more than half of
these patients will demonstrate RF in synovial fluid.
However, if RF is only being produced by joint tissue,
synovial fluid RF may be positive while the serum RF
is negative.4 False-positive RF can result from other
chronic inflammatory diseases.6
MICROSCOPIC EXAMINATION
OF SYNOVIAL FLUID
Cell Counts
Synovial fluid cell counts, as all body fluid cell counts, should
be performed within 1 hour of collection. Hemocytometer
counts and manual differentials are normally performed on
synovial fluid. Saline may be used as a diluent for synovial
fluids with a high number of cells. Hypotonic saline, a weak
acid, or commercially available white blood cell (WBC) diluent reservoirs may be used when many RBCs are present.
Instruments are available to automate these counts (see
Chapter 15). Cytocentrifugation of the specimen provides
good smears for Wright staining and observation.
Figure 11-8. Mucin clot test of normal synovial fluid.
accompany Crohn disease, gout, psoriasis, Reiter syndrome, and ulcerative colitis.2
Glucose. Synovial fluid glucose levels should be interpreted using serum glucose levels. A fasting specimen
should be used or at least one 68 hours postprandially. Normally, synovial fluid glucose levels are less
than 10 mg/dL lower than serum levels. Joint disorders that are classified as infectious demonstrate large
decreases in synovial fluid glucose and can be as much
as 20100 mg/dL less than serum levels. Other groups
of joint disorders demonstrate a less of a decrease in
synovial fluid glucose, 020 mg/dL.2
Uric acid. Synovial fluid uric acid normally ranges from
6 to 8 mg/dL. The presence of uric acid in synovial
fluid is helpful in diagnosis gout. Usually, crystal identification is used for this determination, but synovial
fluid uric acid levels may be performed in laboratories
that do not a have light polarizing microscope.4
Lactic acid. Lactic acid is rarely measured in synovial
fluid but can be helpful in diagnosing septic arthritis.
Normally, synovial fluid lactate is less than 25 mg/dL
but can be as high as 1000 mg/dL in septic arthritis.4
Lactate dehydrogenase. Lactate dehydrogenase (LD) can
be elevated in synovial fluid, while serum levels remain
normal. Synovial fluid LD levels are usually increased
in RA, infectious arthritis, and gout. The neutrophils
Differential
Normal synovial fluid contains small numbers of lymphocytes and only a few neutrophils (Fig. 11-9).
The WBC count on normal synovial fluid ranges from 0 to
150 cells per microliter. The mean distribution of these nucleated cells is neutrophils 7%, lymphocytes 24%, monocytes
48%, macrophages 10%, and synovial lining cells 4%.1 The
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are seen in synovial fluid in about 10% of patients with systemic lupus erythematosus and in some patients with RA
(Fig. 11-11). LE cells are neutrophils that have engulfed a
nucleus of a lymphocyte that has been altered by antinuclear
antibody. Tart cells, monocytes that have engulfed nuclear
material (Fig. 11-12), may be confused with LE cells.
Although not specific for Reiter syndrome, Reiter cells may
be present in synovial fluid. Figure 11-13 shows a Reiter cell
(neutrophil-laded macrophage).
Lipids may be released from bone marrow after injury to
the bone. As a result, lipophages as seen in Figure 11-14
may be present in synovial fluid.1
Crystals
Examination of synovial fluid for crystals is a routine test in
most laboratories. Crystal analysis is most commonly used to
diagnose gout by the presence of monosodium urate (MSU)
crystals. Chapter 8 contained an explanation of polarization
and compensation of light in the analysis of crystals. MSU
crystals that appear in synovial fluid are usually thin, needlelike crystals. MSU crystals polarize light and are negatively
birefringent (crystals aligned with the compensator filter are
Figure 11-13. Reiter cell (center) is a macrophage that has phagocytosed one or more neutrophils. This finding is not specific for Reiter syndrome. (From McClatchey KD. Clinical Laboratory Medicine. 2nd Ed.
Philadelphia: Lippincott Williams & Wilkins, 2002.)
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260
Table 11-2
GROUP
CATEGORY
VISUAL
VISCOSITY
MUCIN
CLOT
Normal
Colorlessstraw
Clear
High
Good
150 WBCs
25% neutrophils
Noninflammatory
Yellow
Slightly cloudy
Decreased
Fair
1,000 WBCs
30% neutrophils
CELL COUNT
GLUCOSE
BLOOD: SF
OTHER
010
010
II
Inflammatory
Absent
Poor
100,000 WBCs
50% neutrophils
04
III
Septic
Absent
Poor
50,000200,000 WBCs
90% neutrophils
20100
Positive
cultures
IV
Crystal induced
White
Cloudy, turbid,
opaque, milky
Absent
Poor
500200,000 WBCs
90% neutrophils
080
Crystals
present
Hemorrhagic
Sanguinous,
xanthochromic,
red, or brown
Cloudy
Absent
Poor
5010,000 WBCs
50% neutrophils
020
RBCs
present
Microbiologic Examination
Infectious agents that can enter the synovial fluid include
bacteria, fungi, Mycobacteria, and viruses, with bacteria
being the most common. Bacteria and other microorganisms enter the synovial capsule through the bloodstream,
deep penetrating wounds, and rupture of osteomyelitis into
the joint. In addition, bacteria may be introduced during
procedures such as arthroscopy, intra-articular steroid
injections, and prosthetic joint surgery.2
Gram stain is performed on synovial fluid smears prepared by centrifugation or cytocentrifugation. Diluting
synovial fluid with saline helps separate cells that tend to
cluster. Even if Gram staining does not suggest the presence
of infectious agents, both aerobic and anaerobic cultures
should be performed. Synovial fluid Gram stains are positive in only 50% of cases with joint sepsis.1,2
Summary
Synovial fluid analysis is a well-established procedure in the
evaluation of joint disease. The purpose of synovial fluid
analysis is to determine the presence of arthritis and to place
a fluid into one of several categories. Appropriate treatment
of joint disease depends on proper identification of disease.1
STUDY QUESTIONS
1. The word synovial means resembling:
a. an oval
b. egg albumin
c. lipids
d. serum
2. Aspiration of joint fluid is indicated for any patient
with:
edematous joints
inflamed joints
painful joints
all of these
a.
b.
c.
d.
ence of:
a. arthritis
b. fibrinogen
c. hyaluronate
d. inflammation
5. No formation of a string when dispensing synovial
Normal
Group I
Group II
Group III
Group IV
Group V
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apatite
calcium pyrophosphate
corticosteroid
monosodium urate
CASE STUDIES
Case 11-1
knees after a fall while skiing. The images below show the results of an
arthrocentesis performed in the emergency department a few days later.
45% neutrophils
10. ________ yellow, cloudy, 80,000 WBCs, 85%
neutrophils
11. ________ yellow, purulent, 220,000 WBCs, 98%
neutrophils
12. ________ xanthochromic, 10,000 WBCs, 30%
neutrophils, erythrophagocytosis
Match the cell with its description.
A.
B.
C.
D.
LE cell
RA cell
Reiter cell
Tart cell
nucleus
16. ________ neutrophil containing immune complexes
Figure 11-19. First image for Case Study 11-1. Synovial fluid.
262
Figure 11-20. Second image for Case Study 11-1. (From McClatchey
KD. Clinical Laboratory Medicine. 2nd Ed. Philadelphia: Lippincott Williams &
Wilkins, 2002.)
Case 11-2
REFERENCES
1. Gatter RA, Schymacher HR. A Practical Handbook of Joint Fluid
Analysis. 2nd Ed. Philadelphia: Lea & Febiger, 1991.
2. McBride LJ. Textbook of Urinalysis and Body Fluids: A Clinical
Approach. Philadelphia: Lippincott, 1998.
3. Brunzel NA. Fundamentals of Urine and Body Fluid Analysis. 2nd Ed.
Philadelphia: Saunders, 2004.
4. Ross DL, Neeley AE. Textbook of Urinalysis and Body Fluids. New York:
Appleton-Century-Crofts, 1983.
5. Strasinger S, DiLorenzo MS. Urinalysis and Body Fluids. 5th Ed.
Philadelphia: FA Davis Company, 2008.
6. Kjeldsberg CR, Knight JA. Body Fluids Laboratory Examination of
Cerebrospinal, Synovial, and Serous Fluids: A Textbook Atlas. Chicago,
IL: ASCP Press, 1982.