This document describes an experiment to test for the presence of starch in leaves. The procedure involves boiling a leaf in water to rupture cell membranes, then boiling it in ethanol to remove chlorophyll. The leaf is then tested with iodine solution, which turns blue-black in the presence of starch. The results are observed and discussed. Key points made include how boiling prepares the leaf for testing, and that a positive test indicates starch is present as a product of photosynthesis.
This document describes an experiment to test for the presence of starch in leaves. The procedure involves boiling a leaf in water to rupture cell membranes, then boiling it in ethanol to remove chlorophyll. The leaf is then tested with iodine solution, which turns blue-black in the presence of starch. The results are observed and discussed. Key points made include how boiling prepares the leaf for testing, and that a positive test indicates starch is present as a product of photosynthesis.
This document describes an experiment to test for the presence of starch in leaves. The procedure involves boiling a leaf in water to rupture cell membranes, then boiling it in ethanol to remove chlorophyll. The leaf is then tested with iodine solution, which turns blue-black in the presence of starch. The results are observed and discussed. Key points made include how boiling prepares the leaf for testing, and that a positive test indicates starch is present as a product of photosynthesis.
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you cyaa chat to me (Repeat Chorus) Testing a Leaf for Starch Apparatus/Materials bunsen burner, tripod stand, wire gauze(OR 90oC electric water bath OR hot plate) 250 cm3 beaker boiling tube anti- bumping granules forceps test tube holder white tile leaf to be tested (hibiscus leaves are excellent) 90% ethanol iodine/potassium iodide solution
Add a few drops of iodine/potassium
iodide solution to the leaf and note any observations. Observations/Results The leaf was flaccid (soft) after being boiled in water The ethanol changed from colorless to green The leaf was brittle after being boiled in ethanol The leaf become flaccid once more after being rinsed in cold water After iodine solution was added... Observation No color change (Iodine Solution remains brown) Iodine/Potassium Iodide solution changed from brown to blue- black
Procedure The video above shows the steps in Testing a Leaf for the Presence of Starch. The Procedure outlined below is slightly different.
Interpretation
Remove a green leaf from a plant that
has been exposed to sunlight for a few hours Half-fill a 250cm3 beaker with water. Heat the water until it boils. Keep the water at boiling point. Use the forceps to place the leaf in the boiling water. Boil for 2 minutes. Turn off the Bunsen Burner. (If you are using a heat source without a naked flame - electric water bath or hot plate - this step is unnecessary.) Place the boiled leaf in a boiling tube containing 90% ethanol. Place the boiling tube in hot water and boil for 10 minutes or until the leaf decolourizes. ( It may be necessary to replace the ethanol) Gently remove the leaf and wash with a fine trickle of cold tap water. Spread the leaf evenly on a white tile.
Describe Photosynthesis. Use a symbol
equation. Photosynthesis is the process by which light energy is harvested by chlorophyll and used to convert inorganic raw materials - carbon dioxide and water - to products glucose and oxygen. Most photosynthesis takes places in the green parts of plants and more specifically, in the leaves. Photosynthesis involves 2 stages - the Light-dependent Stage and the Lightindependent stage. In the lightdependent stage, water is photolyzed by sunlight into Hydrogen and oxgen. Hydrogen enters the second stage, oxgen is gaseous by-product.
Starch is not present. Starch is present
Discussion Questions & Answers
In the light - independent stage
enzymes catalyse the reduction of carbon dioxide by hydrogen. The
carbohydrate, glucose is first formed.
Glucose may be immediately used, translocated in the form of fructose or sucrose or stored as starch granules. The overall equation for photosynthesis is: Picture Why is starch tested for and not glucose? Glucose is the product of photosynthesis, and is rapidly converted to granules of starch - a polymer of glucose - for storage. Starch granules have been visualized in the stroma of the chloroplast as well as the cytoplasm. It is advantageous to the plant to convert glucose to starch. Starch is insoluble in water and therefore not a solute in the solution component of the stroma. Glucose on the other hand is soluble in water and if it accumulated in the stroma, the stroma would become more concentrated hypertonic - relative to the cytoplasm. Water would move from the cytoplasm ( higher water potential) via osmosis into the stroma of the chloroplasts (lower water potential), causing them to swell and burst. . What observations will you make if the test is positive? The iodine solution will turn from brown to blue - black. Explain the significance of boiling the leaf in water. Boiling the leaf in water Removes the waxy cuticle which prevents entry of iodine/potassium iodide solution. Ruptures cell membranes to make starch granules in cytoplasm and chloroplasts accessible to iodine/potassium iodide solution. Cell membranes are selectively permeable
and do not readily allow the
penetration of iodine. Denatures enzymes, particularly those which convert starch to glucose e.g. diastase. Boiling arrests all chemical reactions, since enzymes which catalyse the reactions are denatured. Denatured enzymes have altered or destroyed active sites due to heat, pH, ionic concentration Account for the texture of the leaf after being boiled in water. The leaf was flaccid (soft) as cell membranes were ruptured and turgor pressure was lost. Explain the significance of boiling the leaf in ethanol Chlorophyll is a green pigment and so masks the colour change of the iodine test for starch. Chlorophyll needs to be removed from the leaf i.e. the leaf needs to be ' decolourized' for changes to be observed. A decolourized leaf is pale yellow or green. Ethanol is an organic solvent and so extracts chlorophyll from the leaf. Account for the texture of the leaf after being boiled in ethanol. The leaf was brittle as ethanol dehydrated (extracted water) from the leaf. Explain the significance of rinsing the leaf in water. The leaf was rinsed in water to rehydrate it. Iodine solution is an aqueous solution of iodine/potassium iodie - potassium tri-iodide; water is needed inside the leaf to enable penetration by diffusion. State whether starch is present Starch is present OR Starch is not present.
Which products of photosynthesis may
be present but not revealed by the iodine test? Glucose, fructose and sucrose are all carbohydrate products of photosynthesis not revealed by the iodine test for starch. Do your results indicate that starch is the first product of photosynthesis? The results do not indicate whether starch is the initial, intermediate or final product of photosynthesis. How can this experiment be extended to demonstrate conclusively that photosynthesis has taken place?
We do not know whether starch has
accumulated in the leaf as a result of photosynthesis or whether the starch is permanently present in the leaf. To improve this experiment, the plant needs to be de-starched, and the leaves tested for the presence of starch. Once it is demonstrated that all starch has been utilized or removed, photosynthesis is allowed to take place by exposing the plant to sunlight. The leaves of the plant are now tested for the presence of starch. If starch is present, then it has been synthesized by photosynthesis. We can then conclude that the presence of starch indicates that photosynthesis has taken place.