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ISSN : 0972-995X
1Department o f Zoology, H.P. T. Arts and R. Y.K. Science College, Nashik (M .S.)
2Department o fZoology, D.B.F. Dayanand College o fArts and Science, Solapur (M .S.)
ABSTRACT : The sub-lethal concentration of the active principal of two piscicidal plants viz. Cestrum nocturnum
(2.8 mg-1) and Cestrum diurnum (3.2 mg-1) induced the haematological changes in a freshwater fish Cyprinus carpio.
Behavioural activities such as erratic swimming, loss of reflex, hyperventilation increased surfacing and jerk move
ments were observed. The total num ber of erythrocytes, leukocytes, and haemoglobin concentration and packed cell
volume declined while erythrocytes sedimentation rate and related param eters like M CH, M C H C , MCV altered with
exposure. Since the plant toxins influence cell integrity and hemopoiesis, the oxygen carrying capacity of blood was
decreased, consequently thermodynamic and metabolic activity in fish was disturbed, therefore Cestrum species seems
to be Karyocytophilic and Cytokaryophilic toxins to the fish.
Key words : Cestrum nocturnum, Cestrum diurnum, Cyprinus carpio, Piscicidal plants.
INTRODUCTION
Received 01.04.2010
Accepted 05.06.2010
82
Table 1 : Mean values of body length, body weight and various haematological parameters of control
and treated individuals of Cyprnus carpio exposed to sublethal concentration of
C.nocturnum (2.8 mg-1) and C.diurnum (3.2 mg-1) for 48 hours.
Parameter
Body length (cm)
Body wt. (gm)
Hb (gm/100 ml)
PCV (%)
MCH (pg)
MCHC (gm/lit)
MCV (um3)
Total no. of RBC (1000/mm3)
Total no. of WBC (1000/mm3)
ESR (mm/h)
Control
13.23 0.20
26.39 0.00
12.8 0.02
36.07 0.03
45.28 0.76
33.28 0.12
132.27 0.54
4.72 0.36
4.30 0.02
1.8962 0.063
C.nocturnum
C. diurnum
13.40 0.42
28.61 0.00
4.40 0.04
18.02 0.05
119.69 0.34
26.28 0.09
412.40 0.59
2.32 0.27
2.89 0.28
3.79 0.072
12.14 0.01
24.40 0.07
6.20 0.02
22.58 0.12
102.39 0.02
23.28 0.02
422.10 0.72
3.03 0.18
3.32 0.42
3.01 0.081
ACKNOWLEDGEMENTS
Authors are thankful to the Head, Department of Zool
ogy, Dr. B. A. Marathwada University, Aurangabad and Prin
cipal of H.P.T. Arts and R.Y.K. Science College, Nashik for
providing necessary facilities.
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