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REVIEW ARTICLE
Department of Microbiology
and Molecular Cell Sciences, The
University of Memphis,
Memphis, TN 38152
2
Department of Animal Science,
University of Illinois, Urbana, IL
61801, U.S.A.
(Received 16 May 1997,
accepted 16 May 1997)
Introduction
Robert E. Hungate was the pioneer in studying rumen
and anaerobic microbiology. He developed the roll
tube anaerobic technique used to culture anaerobic
bacteria. His work not only contributes to the understanding of the complicated microbial ecosystem of
the rumen, but also paves the way for investigations of
vast number of anaerobic microorganisms, which are
an important part of general microbiology.
Robert E. Hungate was born on March 2, 1906 at
Cheney, Washington. His father was a biologist who
taught at the State Normal School (now Eastern
Washington State University) for almost 50 years. As a
child Hungate enjoyed outdoor activities, mounting
insects and skins of small animals and birds for
museum preparation. Young Robert graduated from
the Normal School (a 2-year college at that time) and
through his father developed a strong interest in the
plant and animal life of the Cheney area and of
Address correspondence to: Dr. King-Thom Chang.
1075-9964/97/040213 + 05 $25.00/0/an970109
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Rumen Microbiology
Hungate stayed in Texas for about 10 years, undertaking numerous investigations, such as disproving
the viral etiology of mouse mammary carcinoma and
participating in the Alpha Chapter of the Society for
Applied Piscatology (SAP). He also carried a full load
of teaching (which he enjoyed profoundly all his
life).
At the end of the World War II in 1945, Hungate
accepted the offer to join the Bacteriology Department
at Washington State College and was soon busy
preparing lectures in Bacteriology! This was quite new
to him as he had taught zoology and an honors
program in general biology while at Texas. Research
continued to be focused on the microbial activities of
the rumen. This was and is important since rumen
microorganisms convert plant materials, including
cellulose, into nutrients for the animal, resulting in
milk, meat and other products used by mankind. This
process urgently needed study.
Hungate was one of the most devoted scientists
ever known who dedicated his effort to the under-
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Personal Caliber
With the stimulation of Hungate methods, the complete analysis of many anaerobic microbial ecosystems
became possible. There are so many habitats which are
free of oxygen in the world, such as human and
animal gastrointestinal tracts, sludges, sediments and
tundra and other environments with reduced oxygen.
Anaerobes constitute 90% or more of the total microbial population of each of these habitats. Without
Hungates anaerobic technology to make their microbial culture possible, our knowledge would be handicapped to only knowing the limited amounts of
aerobes and facultative anaerobes culturable by the
common aerobic media. One typical example is
Escherichia coli, which is not by any means a major
intestinal bacterium. But its name is so well known
that many have the misconception of forgetting the
anaerobes! Anaerobic technology has been extended
to other fields such as human intestinal microbiology;
this has made a great progress with a profound impact
on human health. Another example is the field of
methanogenesis. Hungate was interested in methanogenesis in the rumen for a long time. Hungates
laboratory at Washington State University was the
first to isolate methanogens using H2-CO2 as energy
source. His anaerobic technique is the most reliable
method for growing methane bacteria. Tremendous
progress in the field of methanogenesis including
molecular biology of methanogens is becoming possible. We should by no means forget to credit him for his
pioneering work.
In addition to his scientific contributions, Hungate
also generously served in various organizations. He
was a charter member of the Texas Branch of the
Society of American Bacteriologists. He is also a fellow
of the American Association for the Advancement of
Science (AAAS), the American Society of Zoologists,
the Northwest Scientific Association, the Society of
Protozoologists, the American Academy of Microbiology, American Academy of Arts and Sciences, and
the American Society for Microbiology. He has not
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