Professional Documents
Culture Documents
requirements according to
ISO/IEC 17043
Dr.-Ing. Frank Baumeister
TGZ AQS-BW
at Institute for Sanitary Engineering, Water Quality and Solid Waste Management
University of Stuttgart
AQS Baden-Wrttemberg
Bandtle 2
70569 Stuttgart
GERMANY
Tel.: +49 711 685 65442 / Fax: +49 711 685 55442
E-Mail: frank.baumeister@aqsbw.de
x1
x2
x3
xt,k:
x1,1 x1,2
x2,1 x2,2
x3,1 x3,2
w1
w2
w3
x1
x2
X3
wt
x
sx
(x
sw
w t /(2g )
ss sx (sw / 2)
x
)
/(g 1)
t ,.
.,.
2
1 ss
2
IUPAC homogeneity
Cochran-Test (I)
190
189
188
187
Result
186
185
Result 1
184
Result 2
Dmax
183
D4
182
181
180
179
0
10
11
12
13
14
15
16
Sample
1.
D
i 1
10
D max
SDD
2
i
IUPAC homogeneity
Cochran-Test (II)
3. Compare the ratio with the appropriate critical value from tables
m
7
8
9
10
11
12
13
14
15
16
17
18
19
20
11
95%
0,727
0,68
0,638
0,602
0,57
0,541
0,515
0,492
0,471
0,452
0,434
0,418
0,403
0,389
99%
0,838
0,794
0,754
0,718
0,684
0,653
0,624
0,599
0,575
0,553
0,532
0,514
0,496
0,48
IUPAC homogeneity
Cochran-Test (III)
Delete before ANOVA at
99% confidence level
190
189
188
187
Result
186
185
Result 1
184
Result 2
183
182
181
180
179
0
10
11
12
13
Sample
12
14
15
16
IUPAC homogeneity
Cochran-Test (IV)
Delete complete data set
192
190
Result
188
186
Result 1
Result 2
184
182
180
178
0
10
11
12
13
14
Sample
13
15
16
IUPAC homogeneity
Cochran-Test (V)
No elimination in the case
of outlying mean value
190
189
188
187
Result
186
185
Result 1
184
Result 2
183
182
181
180
179
0
10
11
12
13
14
Sample
14
15
16
15
2
Sum of the squared differences: s Di / 2m
i 1
equivalent to within-sample standard deviation
2
an
v S (S i S ) /(m 1)
Variance Vs of the sums Si:
I 1
equivalent to the standard deviation of the sample average
2
(Vs / 2 san ) / 2
Sampling variance: ssam
equivalent to between-sample standard deviation
16
m*
F1
20
1,59
19
1,6
18
1,62
17
1,64
16
1,67
15
1,69
14
1,72
13
1,75
12
1,79
11
1,83
10
1,88
9
1,94
8
2,01
7
2,1
F2
0,57
0,59
0,62
0,64
0,68
0,71
0,75
0,8
0,86
0,93
1,01
1,11
1,25
1,436
17
Free software...www.aqsbw.de
Homogeneity check Example from ISO
(ISO 13528 Annex B)
13528
value#2, xt,2
Sample t value#1, xt,1
1
10,5
2
9,6
3
10,4
4
9,5
5
10
6
9,6
7
9,8
8
9,8
9
10,8
10
10,2
11
9,8
12
10,2
13
14
15
16
17
18
19
20
number of samples g
10,4
9,5
9,9
9,9
9,7
10,1
10,4
10,2
10,7
10
9,5
10
Homogeneity check
(Intern. Harmonized Protocol
Appendix 1)
Sample t
value#1, xt,1
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
10,5
9,6
10,4
9,5
10
9,6
9,8
9,8
10,8
10,2
9,8
10,2
value#2, xt,2
10,4
9,5
9,9
9,9
9,7
10,1
10,4
10,2
10,7
10
9,5
10
D=a-b
S=a+b
20,9
19,1
20,3
19,4
19,7
19,7
20,2
20
21,5
20,2
19,3
20,2
0,1
0,1
0,5
-0,4
0,3
-0,5
-0,6
-0,4
0,1
0,2
0,3
0,2
D=(a-b)
0,01
0,01
0,25
0,16
0,09
0,25
0,36
0,16
0,01
0,04
0,09
0,04
(S i S ) 2
0,736736
0,886736
0,066736
0,411736
0,116736
0,116736
0,025069
0,001736
2,126736
0,025069
0,550069
0,025069
Homogeneity check
12
10
10,02083333
0,340092456
0,247487373
0,291612549
Analytical result
12
8
1st result
2nd result
4
2
1,14
0
0
10
15
20
Sample
Homogeneity
ok
number of samples
12
Cochran test procedure for duplicate results
2
Dmax
0,36
SDD
1,47
C
0,24489796
critical value (99%)
0,653 no outlying pair
critical value (95%)
0,541 no outlying pair
Homogeneity check
11
10,8
Analytical result
10,6
10,4
1st result
10,2
2nd result
10
9,8
9,6
9,4
0
10
15
20
25
Sample
18
1,14
critical F1
1,79
0,26204056
critical F2
0,86
Homogeneity
OK
Dr. Michael Koch, AQS Baden-Wrttemberg, Universitt Stuttgart
0,116964
Serum
Fuji dri-chem 3500i
CRP (C-reactive protein)
Quantitative
Fuji
2500
11%
Code
13-06
656
700
719
CR
Duplicates
Sample
1
2
3
4
5
6
7
D.1
370,000
360,000
380,000
430,000
400,000
420,000
430,000
D.2
400,000
410,000
410,000
390,000
420,000
390,000
380,000
ISO_IUPAC_Serum_13-06.xlm
Serum
Fuji dri-chem 3500i
CK-MB (Creatine kinase)
Quantitative
Fuji
50
25%
656
32
13-09
Duplicates
Sample
1
2
3
D.1
44,00
45,00
43,00
D.2
42,00
41,00
39,00
ISO_IUPAC_Serum_creatine.xls
Urine
Urisys 1800
Protein
Parameter type
Semi-quantitative
Method
Strips
Prepared samples 550
Code
Sample
1
2
3
12-04
Measurement
D.1
+++(2-5g/L)
+++(2-5g/L)
+++(2-5g/L)
577
135
409
U2
Number of samples is
definitly too low
Evaluation of homogeneity
only by comparison of the
results possible
No statistical tool available
Urine
Tox/see
Opiates
Categorical
200
Code
13-10
Measurement
D.1
Positive
Positive
Positive
Sample
1
2
3
Value 1
12,1
9,6
10,4
9,5
10
9,6
9,8
9,8
10,8
10,2
9,8
10,2
Value 2
10,4
9,5
9,9
9,9
9,7
10,1
10,4
10,2
10,7
10
9,5
10
= 1,14
ISO_IUPAC_soya_flour.xls