Professional Documents
Culture Documents
1.
(a)
(b)
(i)
1
1
2 max
[5]
2.
(a)
(b)
(c)
3.
(a)
OR
tertiary shape;
locks onto / complements specific antigen;
(b)
(c)
5 hrs or more;
small vols. / water must be clear /
full sunlight needed if water required quickly / shortage of containers;
max.2
1
1
(d)
(a)
safer to catch some diseases when young; (reject immunity lasts longer)
example of side effect suffered if disease contracted by adult
2
consider vaccines to be unsafe / have side effects / damage immune system;
consider natural immunity to be more effective; allow in (a) if not here
religious / ethical objections qualified e.g. objections to use of fetal /
animal tissue;
consider low risk of disease when high percentage of population already
vaccinated/Ref. to Head Effect
max 2
[7]
4.
(b)
(c)
(d)
max 3
[12]
5.
(a)
amino acid;
(b)
(c)
(d)
1 max
[4]
6.
(d)
2
1
2
7.
(a)
(b)
(c)
2
1
max 6
2
2
2
[15]
8.
(a)
(b)
2 max
4
[7]
9.
(a)
(b)
3
[5]
10.
(i)
1360 = 2 marks
(general principle 0.68 0.05 x 100 gains 1 mark)
(ii)
1
[3]
11.
(a)
(a)
(c)
(i)
(ii)
Weakened organism;
(ii)
(d)
2 max
3600;
No memory cells;
Short-term / not lifelong;
Antibodies (or context established) donated by mother /
across placenta / in milk;
2 max
[8]
12.
(a)
(b)
1
2
3
4
5
6
restriction enzyme/endonuclease;
to cut plasmid/to form sticky ends in plasmid;
(use) ligase(to join) gene to plasmid;
culture bacteria with(in medium containing) plasmids
to allow uptake of plasmids / transformation;
use of cold shock/chemical treatment(to enhance uptake)/ heat
shock;
(ignore bullets/electroporation /microinjection)
4 max
1
2
3
4
5
6
3 max
[7]
13.
(a)
(b)
add antibodies/enzyme;
wash to remove unbound antibodies;
add (colourless) solution;
(mark correct responses sequentially)
antibodies specific/shape only fits one antigen;
other antigens different shape;
would not bind to antibodies;
2 max
[5]
14.
(a)
(b)
(i)
Molecule/part of molecule/protein/glycoprotein;
[Allow: polysaccharide]
Stimulates immune response;
(ii) These antigens/antibodies have complementary/particular shape;
[Reject: Active site]
Allow fitting/binding with (relevant) antibody/antigen;
Calichaemicin delivered specifically to cancer cells/less likely to/will
not harm normal/healthy cells;
Lower dose of calichaemicin needed to be effective;
2
2
2
[6]
15.
(a)
(i)
(ii)
(b)
1.
2.
3.
4.
5.
protein/immunoglobulin;
specific to antigen;
idea of fit/complementary shape;
1.
virus contains antigen;
2.
virus engulfed by phagocyte/macrophage;
3.
presents antigen to B-cell;
4.
memory cells/B-cell becomes activated;
5.
(divides to) form clones;
6.
by mitosis;
7.
plasma cells produce antibodies;
8.
antibodies specific to antigen;
9.
correct reference to T-cells/ cytokines;
antibody gene located using gene probe;
cut using restriction enzyme;
at specific base pairs;
leaving sticky ends/unpaired bases;
cut maize/DNA /vector using same restriction enzyme;
2 max
6 max
6.
7.
(c)
passive;
person is not making own antibodies/antibodies not replaced;
memory cells not produced;
(d)
(a)
(i)
4 max
2 max
1
[15]
16.
(ii)
(b)
2
[5]
17.
(a)
(b)
5 max
2
[8]
18.
(a)
47 213;
(b)
(i)
(ii)
(c)
2 max
[5]
19.
(a)
(b)
(c)
(d)
max 2
[7]
20.
(a)
(b)
(c)
(i)
(ii)
(d)
Mitosis;
Identical genetic material/DNA passed to daughter cells;(ignore
reference to chromosomes)
DNA/gene codes for protein;
Antibody is a protein;
Antibodies;
passed from mother in breast milk/across placenta;
2
[10]
21.
(a)
(b)
(c)
acts as an antigen;
stimulates immune response/production of antibodies;
production of memory cells;
which rapidly produce antibodies when individual infected by Hib;
max 2
max 2
killed microorganism;
modified toxin;
attenuated/heat treated/UV treated microorganism;
genetically engineered antigens;
isolated antigen;
max 2
[6]
22.
(a)
(b)
max. 5
action of phagocytes;
Interferon production;
body temperature increased;
ref to B or T lymphocytes;
activated by non-self antigen;
either clone / divide by mitosis;
T helper cells role;
B plasma cells produced;
which produces antibodies;
any specific effect (e.g. immobilise /agglutinate / lysis /coat for recognition /
neutralise toxins);
T killer / cytotoxic cell;
perform produced;
memory cell produced;
max 7
[12]
23.
(i)
(ii)
antibody present;
(iii)
2
[4]