Professional Documents
Culture Documents
SEM 1 2006/2017
PREPARED BY
HJH ROSLINDA SESWOYA
General Requirements
1. Be properly prepared to do the experiment scheduled for that day.
2. There will be no smoking, using CELL PHONES, drinking, chewing gum, or eating
in the laboratory.
3. Do not touch your face, handle contact lenses, apply cosmetics or put your
fingers in your mouth.
4. Always wash your hands after handling viable material.
5. Avoid working alone in the laboratory.
6. Horseplay will not be tolerated. Avoid distracting others that are working.
7. At the end of each lab session, clean your lab bench, put away all materials,
and wash your hands thoroughly before leaving the laboratory. Wastes should
be deposited in appropriate receptacles.
8. Gloves and lab coats are to be removed prior to leaving the laboratory and may
not be worn in non-laboratory areas.
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Unauthorized experiments are prohibited. Perform the experiments as directed by your instructor.
Never take chemicals, supplies, or equipment out of the laboratory.
Students are not allowed to enter the Chemical Stockrooms.
Know where the MSDS (Material Safety Data Sheets) are for your chemicals. MSDS contain information
that you will need in case there is an emergency.
5. Never taste or smell a chemical.
Learn the locations and operation of emergency equipment. This includes eyewash, fire extinguishers, etc.
Know what to do in case of an emergency.
Know how to exit the building in case of an emergency.
Report ALL accidents, injuries, and near misses (close-calls) to your instructor immediately
Treat burns immediately by putting the burned area under cold water for at least 15 minutes.
Clean up all spills immediately and notify your instructor. This includes water. In the event that a chemical
is spilled/splashed on your eyes, skin or body, IMMEDIATELY rinse the affected area for 15 minutes.
7. If your skin is exposed to a viable material, wash the affected area with antibacterial soap and hot water.
8. In case of accidental ingestion, notify instructor immediately and call for emergency medical services
(Dial 999).
9. Have the MSDS ready for personnel to determine the necessary course of treatment.
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MA01
MA01
Weigh the
record the
initial
dry
weight
of
filter paper
Switch on
the balance
( at least 30
min before
the test)
Weigh and
record the
final
dry
weight
of
filter paper
Pour 15 mL of water
sample ( from UTHM
lake) on the filter paper
and leave it until the
filtration finishes
MA01
Weight (g)
W1
W2
MA02
Bacteria Count
MA02
Bacteria Count
MA02
Note
All equipment used
in this experiments
should be sterilized
in an autoclave at
1210C for 15 min
Carefull with ethanol
and Bunsen burner
Bacteria Count
Inoculate
plate
containing
nutrient agar
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MA02
Bacteria Count
For Environmental Engineering Laboratory ( BFC 32501) SEM 1 2016/2017, submit the
lab report by answering the following
A )Data collection and analysis
1) Complete the table, What is the bacterial count of the sample? Show the value in unit
of bacteria/mL sample.
Bacterial count = Number of colony/ (dilution x volume of sample applied on plate)
Dilution
Count
Countable ( Yes/No)
1/10
1/100
1/1000
1/10000
1/100000
B) Discussion
1) Between pour plate and spread plate,which method is more flexible to be used as
enumeration procedure for various types of bacteria ?
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BOD Bottle ID
Dilution water
( freshly prepared)
Sample
Total
volume
Sample 1
297
300
Sample 2
297
300
Sample 3
297
300
Blank 1
300
300
Blank 2
300
300
Dilution water * = Distilled water + phosphate buffer + magnesium sulfate + calcium Chloride + Ferric chloride
* Read 5210 B (Standard Method) for details
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( Eqn C)
Where
Di = initial DO ( mg/L)
Df = final DO (mg/L) , 5 days or 3 days
P = Volume of sample used / Total volume
Note:
1)
DO in blanks should not deplete more than 0.2 mg/L
2)
DO in sample should not less than 2 mg/L
3)
Final DO in the samples should not less than 1 mg/L
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Initial DO (mg/L)
Final DO (mg/L)
Sample 1
Sample 2
Sample 3
Blank 1
Blank 2
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MA04
JAR Test
18
MA05
19
MA05
3.
4.
5.
6.
7.
- 1.5 mL sample
- 2.5 mL standard potassium dichromate
solution ( 0.04167 M)
- 1.5 mL sulfuric acid reagent
COD Vial
COD Reactor
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To obtain S (mL)
To obtain B (mL)
Do it in fume hood
Do it in fume hood
Do it in fume hood
100 mL flask
Note, this procedure had amended to make it possible within 2 hours Refer
Standard Method 5220 for details of COD measurement.
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Chemical
Oxygen
Demand
(COD):
MA05
For Environmental Engineering Laboratory ( BFC 32501) SEM 1 2016/2017,
submit the lab report by answering the following
Data collection and analysis
1) What is the COD of the sample ? Provide the calculation.
2) In your opinion, is it possible to discharge the sample used in this test to be
discharged to the river?. Give your reason.
Discussion
1) Is it possible to predict the BOD of the sample using the COD value? If yes,
explain how to do that.
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