Professional Documents
Culture Documents
http://biochem158.stanford.edu/
Doug Brutlag
Professor Emeritus of Biochemistry & Medicine
Stanford University School of Medicine
Doug Brutlag 2015
Rheumatoid arthritis
Systemic Lupus Erythematosus
Type 1 diabetes mellitus
Multiple sclerosis
Pernicious anemia
Tom Okarma
- Geron
Doug Brutlag
2015
Tom Okarma
- Geron
Doug Brutlag
2015
Tom Okarma
- Geron
Doug Brutlag
2015
Tom Okarma
- Geron
Doug Brutlag
2015
Tom Okarma
- Geron
Doug Brutlag
2015
Tom Okarma
- Geron
Doug Brutlag
2015
Tom Okarma
- Geron
Doug Brutlag
2015
Tom Okarma
- Geron
Doug Brutlag
2015
Tom Okarma
- Geron
Doug Brutlag
2015
Tom Okarma
- Geron
Doug Brutlag
2015
Judith
DougShizuru
Brutlag 2015
Hematopoiesis
http://www.biocarta.com/pathfles/h_stemPathway.asp
Judith
DougShizuru
Brutlag 2015
Autologous
Autologous
Allogenic
Healthy
Donor
Purify from
Tumor cells
Unfractionated bone
marrow or mobilized
blood
Transplant
Transplant
Donor blood
formation, graft-vstumor effect
Judith
Shizuru
Doug
Brutlag 2015
Judith
DougShizuru
Brutlag 2015
Judith
DougShizuru
Brutlag 2015
Judith
DougShizuru
Brutlag 2015
CD34
Thy-1
Thy-1
c-Kit
Lineage: T, B, Macrophage/Monocyte
Granulocyte, Red Blood Cells
Judith
DougShizuru
Brutlag 2015
Courtesy
Irv Brutlag
Weissman
Doug
2015
Courtesy
Irv Weissman
Doug
Brutlag 2015
101
102
103
104
105
Courtesy
Irv Brutlag
Weissman
Doug
2015
Judith
DougShizuru
Brutlag 2015
Applications of
Hematopoietic Stem Cell Transplantation
Treatment of patients with blood tumors.
Treatment of autoimmune disease
Patients treated with bone marrow transplants are
often cured of autoimmune disease
Bone marrow transplant donors with autoimmune
disease can pass the disease on to recipients
Judith
Shizuru
Doug
Brutlag
2015
MOG
MOG
Judith
DougShizuru
Brutlag 2015
3
2
TLI + ATS
TBI
4
3
1
0
2
1
0
+60
-35
+60
TLI + ATG
HSC
T c e l ls
1 .0 0
B c e ll s
M a c ro p ha g e s
0 .7 5
G r a n u lo c y t e s
0 .5 0
0 .2 5
0 .0 0
0
1 0
2 0
3 0
4 0
5 0
6 0
7 0
T im e ( d a y s )
Judith
Shizuru
Doug
Brutlag 2015
Judith
Shizuru
Doug
Brutlag 2015
80
60
NOD HSC
AKR HSC
NOD
NOD
NOD
40
txp
20
10
12
14
Judith
DougShizuru
Brutlag 2015