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Editorial
The basic LQ equation applies to cell populations of sufficiently homogeneous radiosensitivity. Thus, much of our
research investigated the cell-survival and cell-killing mechanisms with synchronized cells in mitosis (tetraploid) or in G1
phase (diploid) of the cell cycle. Stationary (G0) phase cells
(diploid) were also used in experiments in which it was not
feasible to synchronize the cells. In this model, the a/b ratio
defines the dose at which the different mechanisms contribute
equally to cell killing.
However, most radiobiology research uses asynchronous
populations of normal or tumor cell lines whose survival can also
be characterized with an LQ equation. Although LQ Eq. 1 can be
fitted to such data, the resultant a and b parameters do not
precisely express the underlying mechanisms. Rather, they are
associations of values for subpopulations in which the range of
a values affects the fitted b values and vice versa (5). This is
reflected by a different form of the equation:
The sublesions associated with b0-inactivation (likely singlestrand breaks [6]) repair rapidly at mammalian body temperatures. Thus, to obtain precise values of a and b0, cells are
irradiated at dose rates >10 Gy/min or at 2 C-6 C, such that the
first sublesion of a 2-hit event will not be removed before a second
has been induced. The sources available to most radiobiology
research have dose rates of 1-2 Gy/min at positions convenient for
cell irradiation; thus, low temperatures should be used to obtain
the maximal and true value of b0. This basic LQ equation can be
lnS=DZa b0 D
SZexpaD exp b0 D2
X
Z nx expax D exp b0x D2
LQ 1a
LQ 2
310
References
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Beyond empiricism
311
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