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Department of Food Science, College of Food and Agriculture, United Arab Emirates University, Al Ain 15551, United Arab Emirates
Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Oman
A R T I C L E I N F O
A B S T R A C T
Article history:
Received 4 August 2014
Received in revised form 1 December 2014
Accepted 17 December 2014
Available online 21 February 2015
Characterisation of protein and lipid fractions of fresh camel meat and their associated changes were
investigated during 9 days of refrigerated storage. Camel meat lipids contained palmitic acid (C16:0) as
dominant fatty acid followed by stearic acid (C18:0) and oleic acid (C18:1 n-9). Content of total saturated
fatty acid (SFA) and unsaturated fatty acids were 58.46 and 41.5 mg/100 g, respectively. Total
unsaturated fatty acids were reduced at the end of storage. Lipids underwent rapid oxidation as
indicated by increase in peroxide value (PV) and thiobarbituric acid reactive substances (TBARS).
Decrease in total haem pigment, myoglobin and haemoglobin content with concomitant decrease in
redness (a* value) was noticed during the storage. Protein extractability, solubility, TCA-soluble peptides
and drip loss increased during storage indicating proteolysis and degradation of structural proteins,
which was also evident from SDS-PAGE pattern. This further corroborates with the decrease of textural
parameters like hardness, cohesiveness, springiness and gumminess. Thus, the protein and lipid fraction
of camel meat underwent considerable changes during refrigerated storage. Therefore, the behaviour of
protein and lipid fraction in camel meat during refrigerated storage could provide better understanding
of the processing and storage conditions.
2015 Elsevier Inc. All rights reserved.
Keywords:
Camel meat
Food composition
Proteolysis
Fatty acid analysis
Protein characterisation
Lipid oxidation
Food analysis
Food processing
1. Introduction
With increasing demand for the meat and meat products due to
increasing human population, there is an urgent need to look for
marginal and underutilised options for the supply of red meat, such
as those in semi-arid and arid lands, of which camel meat is
certainly the most suitable one. There are approximately 25
million camels in the world where the global market for camel
products has a potential of US$10 billion a year based of Food and
Agriculture Organisation (FAO) of United Nations. United Arab
Emirates is known to possess 412,000 camels (FAOSTAT, 2008).
Camels are used for many purposes such as meat and milk
production, and for physical labour as well as racing. Camel meat
is known to be more benecial for health because the meat
contains lower fat and cholesterol levels than other red meats
(Gheisari and Ranjbar, 2013). Camel meat is also relatively high in
polyunsaturated fatty acid (PUFA) in comparison to other red
meat (Gheisari and Ranjbar, 2013), which contributes to its healthpromoting benets. Consumption of camel meat can therefore lead
to a reduction in total fat and cholesterol intake and an increase in
PUFA as compared with other conventional meat sources. Moreover,
camel meat is also used for medicinal purposes in diseases such as
hyperacidity, hypertension, pneumonia, and respiratory disease; it is
also known as an aphrodisiac (Kurtu, 2004). Such a diet can be
expected to reduce cardiovascular diseases and improve health
(Rawdah et al., 1994). Thus, camel as a meat source seems to present
a viable alternative to other red meats.
The compositional quality of fresh camel meat can be affected
by problems encountered during refrigerated storage, i.e. lipid
oxidation, discoloration, proteolysis and increase of drip loss, etc.
Camel meat is the least studied meat and is mistakenly believed to
be of lower nutritive value and quality than other types of red meat
(Abdelhadi et al., 2013). Although camel meat is not universally
consumed, it might be a potential alternative for meat, particularly
in arid/semi-arid regions where camels can thrive well and are
produced in a much more economical manner than cattle.
Low-temperature storage is one of the primary preservation
methods to maintain meat freshness, because the rates of
213
2.1. Chemicals
Chloroform, ethanol, acetone and anhydrous sodium sulfate
were obtained from BDH Prolabo (Briare, France). Methanol,
sodium chloride, hydrochloric acid and glacial acetic acid were
obtained from Scharlau Chemicals (Barcelona, Spain). Ammonium
thiocyanate and trichloroacetic acid were procured from Panreac
AppliChem (Barcelona, Spain). Iron (II) chloride and Coomassie
brilliant blue R-250 was procured from AppliChem (Darmstadt,
Germany). Cumene hydroperoxide (80% purity), thiobarbituric acid
(>98% purity), 1,1,3,3-tetramethoxypropane (MDA) (99% purity),
tyrosine (>98% purity), sodium phosphate dibasic, sodium dodecyl
sulfate (SDS) (>98.5% purity), b-mercaptoethanol (b-ME), wide
range molecular weight marker and bovine serum albumin (BSA)
(>98% purity), were procured from Sigma-Aldrich Chemical Co. (St.
Louis, MO, USA). All other chemicals used were of analytical grade.
2.2. Preparation of camel meat samples
Six female camels (Arabian dromedary one-humped camel,
Camelus dromedarius), which have been reared in a semi-intensive
management system and fed ad libitum on a Rhodes grass (Chloris
gayana) hay diet mixed with date seed powder, were slaughtered
at 35 years of age and when they had reached 410 25 kg, at an Al
Ain slaughterhouse in the United Arab Emirates (UAE). UAE-Standard
No. 993/2000 concerning animal slaughtering requirements according to Islamic law was followed to slaughter the camels. Semitendinosus (ST) muscle was carefully separated with a sharp knife from the
carcass of the camels within 24 h of slaughter. Separated meat
portions were packed in polyethylene bags and stored in insulated
box lled with ice during transportation to laboratory of Department
of Food Science, UAE University. Upon arrival, the meat was washed
with chilled deionised water, cut into similar-sized pieces
(3 cm 3 cm 3 cm), and the connective tissue and fat deposits
were removed manually. Meat samples obtained from the carcass of
the six female camels were divided into three batches (or replicates)
and placed on separate polystyrene trays wrapped with shrink lm.
One portion from each batch or replicate was subjected to
214
Drip loss %
"
#
weightsample before cooling weightsample after cooling
weightsample before cooling
100
215
Table 1
Changes in fatty acid composition of lipids extracted from camel meat on day 0 and day 9 of refrigerated storage.
Fatty acids
Common name
0 day
9 day
Saturated FA
C10:0
C12:0
C13:0
C14:0
C15:0
C16:0
C17:0
C18:0
C20:0
Capric acid
Lauric acid
Tridecylic acid
Myristic acid
Pentadecylic acid
Palmitic acid
Margaric acid
Stearic acid
Arachidic acid
0.39 0.094a
0.16 0.069a
0.71 0.084a
5.88 1.98b
1.99 0.13a
29.64 2.74b
1.98 0.15a
16.56 2.15a
1.15 0.097a
0.36 0.090a
0.17 0.071a
0.68 0.088a
6.19 1.99a
1.94 0.12a
30.33 2.95a
1.97 0.14a
16.94 1.95a
1.53 0.099a
Mono-unsaturated FA
C14:1
C15:1
C16:1
C17:1
C18:1 n-9
Tetradecenoic acid
Ginkgolic acid
Palmitoleic acid
Ginkgolic acid
Oleic acid
0.43 0.079a
0.47 0.077a
3.15 1.51a
0.79 0.094b
24.48 2.45b
0.44 0.083a
0.56 0.82a
3.07 1.49a
0.92 0.099a
25.17 2.55a
Poly-unsaturated FA
C18:2n6t
C18:2n6c
C18:3n6
C18:3n3
C20:2
C20:3n3
C20:4n6
C22:2
C22:6n3
2.23 0.25a
3.46 0.26a
2.03 0.198a
1.90 0.12a
0.08 0.006a
0.21 0.007b
1.87 0.118a
0.14 0.008a
0.31 0.009a
2.07 0.23b
3.01 0.25b
1.39 0.198b
1.34 0.106b
0.07 0.004a
0.29 0.009a
1.22 0.119b
0.15 0.009a
0.21 0.008a
Total saturated FA
Total unsaturated FA
Total mono-unsaturated FA
Poly-unsaturated FA
Ratio of n-6/n-3
58.46
41.54
29.32
12.23
3.96
60.11
39.89
30.16
9.75
4.17
Values are mean SD from 3 determinations. Different letters in the same row for each fatty acid at different storage time denote the signicant difference (P < 0.05).
contributed to about 30% of total fatty acids and among them oleic
acid (C18:1n9c) followed by palmitoleic acid (C16:1) were the major
ones. Rawdah et al. (1994) also reported that MUFA in camel meat
account for almost one-third of the total fatty acids and are
dominated by oleic acid followed by palmitoleic acid. Content of
total saturated fatty acid (SFA) and unsaturated fatty acids were
58.46 and 41.5 mg/100 g, respectively, indicating that camel meat
lipids were dominated by saturated fatty acid with considerably high
amounts of unsaturated fatty acids. Among the unsaturated fatty acid,
camel meat lipid contain 29.23 mg/100 g of mono-unsaturated fatty
acids (MUFA) and 12.23 mg/100 g of poly-unsaturated fatty acids
(PUFA). High amount of unsaturated fatty acids (41.54 mg/100 g)
could play a major role in making the lipids more susceptible to lipid
oxidation. The dominant PUFA in the camel meat lipid was linoleic
acid (Cis) (C18:2n6c). Kadim et al. (2013) also reported that the main
polyunsaturated fatty acids in the muscles were linoleic acid
(C18:2n6c). This nding further corroborates with those reported
by Rawdah et al. (1994). These unsaturated fatty acids in camel meat
lipids underwent oxidation during the refrigerated storage for 9 days.
This was evidenced by the decrease in total unsaturated fatty acids
from 41.54 to 39.89 mg/100 g and PUFA from 12.23 to 9.75 mg/100 g
on day 9 of storage. This was in agreement with the high formation of
PV and TBARS in the camel meat on day 9 of storage (Fig.1). Decrease
in PUFA was coincidental with an increase in saturated fatty acids at
the end of refrigerated storage. When comparing fatty acids between
camel meat lipids at day 0 and day 9, there was a noticeable decrease
in some of the PUFA especially C18:2n6, C18:3n6, C18:3n3 and
C20:4n6. PUFA reduction was due to oxidative and hydrolytic
reactions that occurred during the storage. Yi-Chen et al. (2008)
showed that long hydrocarbon chains and high unsaturation of PUFA
made them more susceptible to hydrolytic reactions than the SFA.
Decrease in PUFA resulted in corresponding increase of SFA when
compared to the total fatty acid. Fatty acid composition of meat can
216
b)
8
c
4
a
3
1
0
0
0
c)
d)
Mb
100
95
a
b
90
bc
b
2
85
14
12
0.14
10
8
Hb
0.1
a
c
bc
3
6
Storage me (days)
0.08
0.06
0.04
0.02
0
80
0.12
Haemoglobin (mM)
PV (mg of hydroperoxide/kg
sample)
a)
3
6
Storage Time (Days)
Fig. 1. Changes in peroxide value (a); thiobarbituric acid reactive substances (b); total haem pigment (c) and myoglobin and haemoglobin content (d) in camel meat during
refrigerated storage. Values are mean SD from 18 determinations. Different letter at different storage time indicates the signicant difference (P < 0.05).
217
Table 2
Changes in textural properties and colour values of camel meat during 9 days of refrigerated storage.
Storage time (days)
Hardness (g)
Cohesiveness
Springiness (mm)
Gumminess (g)
Chewiness (mJ)
Textural properties
0
9
3228 39.60a
927.5 23.03b
0.61 0.01b
0.73 0.02a
6.4 0.1a
5.30 0.22b
2235 17.07a
774.1 27.98b
146.39 5.01a
40.13 4.98b
L*
a*
b*
Colour values
0
3
6
9
34.44 0.42c
35.46 1.23c
37.59 1.14a
38.83 0.11b
19.98 1.48a
16.73 0.66b
12.81 0.46c
11.58 0.72d
17.78 1.05a
15.72 0.91b
15.03 0.66cb
14.75 0.04c
For textural properties and for colour, values are mean SD (n = 18). Different letters in the same column for each parameter at different storage time denote the signicant
difference (P < 0.05).
(140 kDa), Alpha actinin (110 kDa), tropomysin (70 kDa), and actin
(55 kDa). C-protein is a thick lament of a single polypeptide chain
having a molecular mass of 140 kDa. a-Actinin is located
exclusively in the Z disc and has a molecular mass of 130 kDa.
Tropomyosin is a protein with two-stranded coiled-coil of an ahelix having a molecular mass of 6570 kDa. Actin is a major
protein of the thin laments in the meat, which comprises 1530%
of myobrillar protein of muscle and had a molecular mass of 43
48 kDa (Abdelhadi et al., 2013). During the storage at refrigerated
temperature for 9 days, different proteins in the camel meat
underwent degradation to some extent as depicted in Fig. 2. MHC,
C-protein, alpha actinin as well as tropomyosin bands showed a
noticeable degradation on day 9 of storage. Proteolytic degradation
of myobrillar proteins, especially myosin, might result in the loss
of structural domains of the meat (Takahashi, 1996), which is
reected by the lower hardness values obtained in the camel meat
on day 9 of storage (Table 2). Ageing or storage of meat at
refrigerated temperature is the period during which an increase
in tenderness and specic degradation of structural proteins
occurs (Kadim et al., 2006). Degradation of different protein
bands in the camel meat during the refrigerated storage might
also be due to the oxidation of proteins as well as due to the
production of endoproteinases by microorganisms. Oxidation of
proteins is believed to cause fragmentation and degradation of
structural protein (Maqsood and Benjakul, 2010b). Proteolysis
by endogenous proteases in the meat during storage might be
associated with the pronounced microbial growth (Maqsood and
Benjakul, 2010b,c). It is well established that the proteolysis of
myobrillar proteins by endogenous proteases during postmortem ageing is primarily responsible for the tenderisation of
meat. The principal degradative change in proteins that occurred
over 9 days of storage, as detected by SDS-PAGE, was the
appearance of troponin T (30KDa) band. Loss of troponin T has
been related to protein degradation and could play a role in
tenderness (Gheisari et al., 2009). Therefore, the camel meat
proteins underwent degradation to some extent during the
refrigerated storage of 9 days.
3.3.2. Changes in protein extractability, protein solubility and TCAsoluble peptides
Extractability of camel meat protein during refrigerated storage
is shown in Fig. 3. Extraction of proteins was carried out in 5% NaCl
as an extraction solvent. Extraction of myobrillar proteins,
commonly called salt-soluble proteins, is crucial in manufacturing
of further processed meat products such as frankfurters and
bologna. Once extracted, these proteins serve as emulsifying
agents in meat batter and contribute to emulsion stability and play
an important role during processing of restructured meat products
(Hultin et al., 1995).
218
Fig. 2. Proteolysis in the camel meat proteins as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
has been used as the index for the protein degradation of muscle
food (Benjakul et al., 1997).
3.4. Changes in quality parameters of camel meat during refrigerated
storage
3.4.1. Changes in pH
Changes in pH of fresh camel meat during 9 days of refrigerated
storage is shown in Fig. 4(a). Initial pH value of fresh camel meat
was 6.2. Soltanizadeh et al. (2008) also reported that the pH of
camel meat was 6.15 after 12 h post-mortem. The range of pH in
the camel meat during the refrigerated storage was from 6.2 to 5.4,
which were within the range for camel meat (6.165.83) reported
by Eskandari et al. (2013). There was an abrupt decrease in pH on
day 3 of storage (P < 0.05), after which the decrease was gradual
until the end of storage period (P > 0.05). This is usually due to the
fact that camels possess high gluconeogenesis capacity because of
the presence of camel hump, which cause rapid drop of pH
(Soltanizadeh et al., 2008). The decline of pH in meat depends on
the amount of glycogen in the muscle at the time of slaughter.
Lower glycogen contents result in decreased rates of anaerobic
glycolysis; therefore, a slower production of lactic acid and hence a
lower rate of post-mortem pH decline (Soltanizadeh et al., 2008).
Camel meat reached to an ultimate pH of 5.4 after 9 days of storage.
The range of the ultimate pH values of dromedary camel meat
ranged between 5.7 and 6.0 (Kadim et al., 2006). The rate and
extent of post-mortem pH decline may induce protein denaturation, affecting tenderness, WHC, juiciness and colour, thus plays
an important role in quality of camel meat.
3.4.2. Changes in textural properties of camel meat
Texture prole analysis of the camel meat at day 0 and 9 of
refrigerated storage is shown in Table 2. Hardness, springiness,
gumminess and chewiness values of camel meat samples sharply
decreased from day 0 to 9 of refrigerated storage, while there was
a)
219
a)
6.5
6.25
pH
b
c
bc
5.75
5.5
5.25
5
b)
3
6
Storage me (Days)
16
14
b)
12
10
8
c
6
4
2
0
0
Fig. 3. Protein solubility (a) and protein extractability and TCA-soluble peptides (b)
in camel meat proteins as affected by refrigerated storage. Values are mean SD
from 18 determinations. Different letter at different storage time indicates the
signicant difference (P < 0.05).
Fig. 4. Changes in pH (a) and drip loss (b) in the camel meat during refrigerated
storage. Values are mean SD from 18 determinations. Different letter at different
storage time indicates the signicant difference (P < 0.05).
220
4. Conclusions
The present study showed that the camel meat contains high
quantities of unsaturated fatty acid with signicant amount of
health-promoting PUFA. However, the presence of PUFA along
with high haem proteins (Hb and Mb) could make camel meat
more prone to lipid oxidation as reected by high PV and TBARS
formation. There was a considerable change in the protein fraction
of camel meat during storage. Degradation of protein bands with
concomitant increase in TCA-soluble peptides and protein
extractability and solubility was evident after 9 days of storage.
Camel meat showed a gradual deterioration of composition quality
attributes, which includes a continuous drip loss and drastic
changes in texture prole and colour. Nevertheless, developing
diverse products from camel meat through optimum processing,
proper preservation and consumer acceptance can be a future
research focus in order to popularise camel meat among the
consumers.
Acknowledgement
Authors are thankful to United Arab Emirates University for the
funding this research through a research grant (No. 31F024).
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