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Hematocrit
Hematocrit test
Hematocrit test
Hematocrit test
Hematocrit test
Hematocrit test:
Measurement
Hemoglobin Determination
Hemoglobin
One of the type of globular protein present in the human
blood
The pigment inside RBCs that has an affinity for O2 (and CO2)
Measured as oxyhemoglobin
Functions of Hemoglobin
Imparts red color to the blood
Helps to carry out the oxygen
Buffers the blood pH
Hemoglobin Determination
measurement of the concentration of hemoglobin in the
blood.
Hemoglobin Determination
Why get tested?
Hemoglobin is measured:
To detect Anemia or Polycythaemia
To monitor the patients response to treatment
Prior to donating blood
To calculate red cells indices
Hemoglobin Determination
METHODS:
COLORIMETERS OR SPECTROPHOTOMETERS
measure the percentage of filtered light transmitted through
the sample
Hemoglobin Determination
METHODS:
HEMOGLOBINOMETER
particularly useful in areas where no clinical laboratories are
available
Hemoglobin Determination
METHODS:
TALLQUIST METHOD
compare the color scale to determine the percent and weight
of hemoglobin in blood under normal and anemic conditions
Hemoglobin
Procedure:
1. Place a drop of blood on the special paper in the Tallquist test kit. The RBCs
break open in the paper.
2. Allow drop to dry long enough to lose its glossy appearance but not long
enough for it to become brown.
Hemoglobin
Procedure:
3. Compare the color of the blood spot with the colors in the Tallquist chart in your
kit. Determine the Hb content by matching colors as closely as possible.
Hemoglobin Determination:
Clinical Significance
Causes of High Hb
Living at a high altitude
Lung disease (emphysema, COPD)
Cancer
Tobacco smoking
Bone marrow disorders (polycythemia vera)
Congenital heart disease in adults
Heart failure
Kidney cancer
Liver cancer
Causes
Nutritional (iron, folic acid, or vitamin B 12 deficiency)
Gastrointestinal blood loss (ulcers, colon cancer)
Kidney problems
Blood loss (from trauma or surgery)
Red Blood cell synthesis problems (bone marrow disorders, genetic
disorders such as sickle cell anemia)
Cirrhosis