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Experiment No
To determine the sulphate content of the given water sample
Principle :
Apparatus:
1) Magnetic stirrer
2) Klett summerson colorimeter or spectro photometer
3) Measuring spoon
Reagent:
a) Conditioning reagent Mix 50 ml glycerol with a solution containing 30 ml
conc. HCL, 300 ml distilled water, 100 ml 95% ethyl alcohol and 75 gm
sodium chloride.
b) Barium chloride crystals AR grade.
c) Standard Sulphate solution Prepare by diluting 10.41 ml of the standard 0.02
NH2SO4
Procedure:
a) Formation of barium sulphate turbidity Measure 100ml
sample or a suitable
Calculation:
BaSO4
SO4Solution
Result :
Experiment No
Aim
Theory :
Reagents
Procedure:
Calculation
:
1000ml of 1N AgNO3 = 35.45gm Cl... 1 ml of 0.0141 AgNO3 = 35.45 x 0.0141mg Cl= 0.499mg Cl- or say 500g Cl- or 0.5mg Cl-
Formula :
(A-B) x 0.5 x1000
-
Mg/l Cl =
ml of sample
Where
Reaction:
A = ml titrant of sample
B = ml titrant for blankV
Cl- + AgNO3 -- AgCl+ NO3
K2CrO4 + 2AgNO3 -- Ag2CrO4 + 2KNO3
Observation Table :
S.N
Type of sample
-
Mg/l Cl =
ml of sample
Result:
Conclusion :
Experiment No
Aim
Theory
Principle :
Apparatus
Reagents
:
i)
ii)
iii)
iv)
v)
Preparation of dilution water: Place required amount of aerated distilled water at 200C. Add 1
ml each of phosphate buffer, magnesium sulphate, calcium
chloride and ferric chloride per liter of water, Seed the dilution
water, if necessary by adding 1 to 10 ml of settled sewage (24
to 36 hours old) per liter (seed should not exert more then 0.5
mg/l of depletion of DO in the blank). Seeded dilution water
should be used the day it is prepared..
Dilution Of sample
Calculations
Reaction
Result :
Conclusion :
Experiment No
Aim :
Theory
Principle
Most types of organic matters are oxidized by the biolling mixtures and
chromic and sulphuric acid . A sample is reflux in strong acid solution with
the known excess amount of potassium dichromate. After digestion the
reaming unreduced K2Cr2O7 is treated with ferrous ammonium sulphate
(FAS )to determine the amount of K2Cr2O7 consumed and oxidasible organic
matter is calculated in term of oxygen equivalent . Mercuric sulphate is
added to remove inference of chlorides. Silver sulphate is added as a catalyst
as it catalyst oxidation of long chain alphatic compounds.
Interference:
Chlorides 1mg/l Cl- exerts 0.23 mg/l of COD. There fore correction as mg/l
Cl- x 0.23 should be applied the COD of Cl- from the total COD. Nitrites
exert COD of 1.1 mg/mgN.
Limitations:
Reagents:
i)
ii)
iii)
iv)
Catalyst Silver Sulphate ( for 8 straight chain sulphatic compds) mercuric sulphate
(for Cl-).
v)
Procedure:
Note: 1. While refluxing if the colour changes to green discard the mixture as
potassium Dichromate is not sufficient to oxidize the solution.
2. For small volumes i.e. 10, 20, 30, 40 ml of samples, proportionate
reduction of potassium Dichromate & sulphuric acid may done)
Calculation:
COD mg/l = (A-B) N x 8000 / V
Where A = Volume in ml. Ferrous ammonium sulphate for blank
B = Volume in ml. Ferrous ammonium sulphate for Sample
V = Volume of sample
N = Normality of ferrous ammonium sulphate