WORKSHEET

TEST PROPERTIES OF ACID BASE SOLUTION WITH

NATURAL INDICATOR
A. Core Competence 1 (KI 1)
Appreciating and practicing the religious teaching which embraced.
B. Basic competence
4.1 Submit ideas on the use of appropriate indicators to determine the acidity of the
acid/alkaline or acid-base titration.
C. Purpose
To prove the characteristic of acid and base solution.

D. Background Information
Indicator solution for pH contain pigments that change colors in acidic and basic
solution. These color changes can be used to determine the relative strength of an acid or base
solution. A pigment found in hibiscus is often called the natural indicator because it can be
used to determine the pH of both acidic and basic solutions. To access the pigment, a small
amount of processing is necessary to produce the hibiscus indicator solution.
The use of Hibiscus flower extract as a naturally occurring acid-base
indicator was studied. This flower contains anthocyanine pigment whose colour depends on
pH. The acid-base equilibrium of this pigment was investigated using spectrophotometric
method. The pk In values were found to be 2.8 and 7.3. The use of this extract in acid-base
titration gave similar result(s) compared to methyl orange indicator.

.
E. Tools and Materials

1. Tools :
watch glass

1 piece

Neraca ohaus

1 piece

Drop pippete
Mortar and pestle

1 piece

Test tube

1 piece

Beaker glass

1 piede

Pelat tetes

1 piece

spatula

1 piece

2. Materials :
Hibiscus flower
Ethanol 70%
Solution with a pH as follows:
No
1
2
3
4
5
6

Solution
HCl 0,1 M
CH3COOH 0,1 M
Asam borat 2%
NaOH
NaHCO3 5%
Na2CO3 0,1 M

pH
1
3
6
7
10
12

D. How do you determine if a solution is an acid or base and its reative strength?
E. Hypotesis
..................................................................................................................................................
F. Identification of Variables
1. Variable Control

: ....................................................................................

2. Variable Manipulation

: ....................................................................................

3. Variable Response

: ....................................................................................

G. Procedure

Making hibiscus extract

1. Prepare tools and Material

2. Plucked hibiscus crown, placed in a watch glass and weighed with a balance mass ohaus

3. Hibiscus put in a mortar

4. crushed hibiscus

5. Put in a beaker glass

6. 10 mL of 70% ethanol was measured using test tube

7. Ethanol 70% put into a beaker containing hibiscus that has been crushed, then mixed and produced hi

Determining acid using hibiscus extract

Adding 2 drops of HCL to cthe first hole of the drop plates

Adding 2 drops of Flower extract to the first hole of The drop plates

Observe the changing Colour

Note the pH value that matches with the colour and determine which one is acid
and which one is base

Determining base using hibiscus extract

Adding 2 drops of NaOH to the first hole of the drop plates

Adding 2 drops of Flower extract to the first hole of The drop plates

Observe the changing Colour

Note the pH value that matches with the colour and determine which one is acid
and which one is base

H. Data Observations Result

No

Solution

pH

Color solution

Color solution after

before natural

natural dropped

dropped indicators

indicators

1
HCl 0,1 M
2
CH3COOH 0,1 M
3
Asam borat 2%
4
NaOH
5
NaHCO3 5%
6
Na2CO3 0,1 M
I. Analysis
1. How does the color of the solution with pH < 7 after dropped natural indicator?
.........................................................................................................................................
2. How does the color of the solution with pH = 7 after dropped natural indicator?
.........................................................................................................................................
3. How does the color of the solution with pH > 7 after dropped natural indicator?
.........................................................................................................................................
4. Judging from the color change, the solution what are classified as acid solution?
.........................................................................................................................................
5. Judging from the color change, the solution what are classified as base solution?
.........................................................................................................................................
J. Conclution
1. Whether the hypothesis is accepted?
.........................................................................................................................................
.........................................................................................................................................
2. What conclusions can be drawn based on the experiment you?
.......................................................................................................................................................
...............................................................................................................................