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ABSTRACT
This study analyzed the evaluation of the antioxidant activity (AOA), total
phenolics, total flavonoids, and total carotenoids of sweet potato (Ipomoea batatas Lam.)
var. Tainong 73 during the drying process. Flesh and skin samples were submitted to
sun-drying, low-temperature drying (LTD) at 25C, hot air drying (HAD) at 50C, or
HAD at 75C, and extracted with ethanol. Then, AOA was assessed by
2,2-diphenyl-1-picrylhydradzyl (DPPH); anti-oxidative potency in linoleic acid system
model (AOP), and 2,2-Azino-di-[3-ethylbenzothiazoline-6-sulfonate] (ABTS) assays.
Skin samples showed, on average, higher AOA than the flesh. The AOA and
composition were higher in samples submitted to HAD at 50 or 75C for four or one day
than at lower temperatures for longer times. Stronger correlation was observed between
the AOA and phenolics than carotenoids content, suggesting that phenolics are the major
contributors to AOA of sweet potato var. Tainong 73.
Key words: antioxidant activity, phenolics, carotenoids, sweet potato, drying process
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Introduction
Food storage is a key factor of food
and nutrition security and food drying by
heat is an old and common practice of
preserving food. It brings food water
activity to a level unfavorable for the
microorganisms development during
storage and facilitates food distribution
by reducing bulk volume. However,
thermal processing has long been
perceived to cause the loss of some
heat-labile nutrients, thus lowering the
nutritional value of the food. Earlier
studies in selected legume sprouts and
seedlings
indicated
that
thermal
processing significantly alters functionality
of legumes (Randhir and Shetty, 2005).
The suitability of a post harvest food
treatment depends on its effect on
nutritious and functional properties of the
food (Marc et al., 2004).
Sweet potato (Ipomoea batatas Lam.)
is an easy-to-grow crop with good
adaptability to diverse environmental
conditions, high yielding ability and high
energy content (Ravindran et al., 1995).
It is suitable for organic food production
and other environmentally friendly
agricultural practices. It ranks fifth most
important food crop in terms of fresh
weight after rice, wheat, corn, and
cassava in developing countries where
95% of its annual production (more than
133 million tons) is concentrated (Centro
Internacional de Potato (CIP), 1998).
Some varieties have anti-carcinogenic
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RDI =
X j ,k X j
j =1
+C ,
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A450 x1000
,
250 xLxW
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y=176.03x3-148.96x2+50.3x, where y is
the water activity and x, the water content
of the samples. The coefficient of
determination was R2= 0.972.
Statistical Analyses
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Table 1. Effect of the Drying Process on the Antioxidant Activity of Sweet Potato var.
Tainong 73
DPPH (%)
A2
AOP (%)
B2
ABTS+23
C2
Treatment1
Flesh
Control
89.260.07b
71.261.80a
4882.240.00a
Sun-drying
86.740.08c 2.82 64.070.00c 10.09
616.520.41c 87.37
LTD at 25C
46.790.39e 47.58 59.180.71c 16.95
616.041.75c 87.38
HAD at 50C 77.330.00d 13.37 68.460.20b 3.93
569.680.00d 88.33
HAD at 75C 90.320.00a -1.19 67.660.00b 5.05
1524.601.82b 68.77
Skin
Control
94.960.14a
82.640.00a
4669.6311.71a
Sun-drying
94.060.08a 0.95 80.440.20a 2.66
1618.024.56d 65.35
LTD at 25C
90.590.04b 4.60 62.871.20c 23.12 1898.970.88c 59.33
HAD at 50C 81.110.12d 14.59 73.650.92b 10.87
622.041.70e 86.67
HAD at 75C
89.100.1b
6.17 74.850.60b 9.67
3224.730.00b 30.94
1
LTD: low-temperature drying; HAD: hot air drying. Means with a same letter in a column are not
significantly different (p>0.05).
2
A, B and C are extent of decrease (%) of DPPH, AOP and ABTS, respectively, during the drying process,
with regard to the fresh samples values.
3
mg vitamin C equivalent /100 g dry weight.
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0.61**
ABTS
0.44*
0.37*
Total phenolics
0.40*
0.63**
0.78**
Total Flavonoids
0.36NS
0.52**
0.31NS
0.64**
0.23NS
0.80**
0.75**
0.16NS
RDI
0.55*
0.63*
0.45*
0.22 NS
0.60*
0.38*
*,** Correlation significant (p<0.05) and (p<0.01), respectively. NScorrelation non-significant (p>0.05).
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Table 3. Effects of the Drying Process on Total Carotenoid Contents of Tainong 731
Total Carotenoids (g -carotene equivalent/g SFW)
Treatment2
A3
Flesh
Skin
B3
Fresh samples
8.460.01a
4.760.01a
Sun-drying
2.410.01d
71.51
1.780.01e
62.61
LTD at 25C
2.700.01b
68.09
3.240.01d
31.93
HAD at 50C
2.540.04c
69.98
3.520.05c
26.05
HAD at 75C
2.190.04e
74.11
3.790.04b
20.37
Means with a same letter in a column are not significantly different ( p>0.05).
A and B are extent of decrease (%) of flesh and skin total carotenoids, respectively, during the drying
process based on fresh samples values.
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Table 4. Effects of the Drying Process on the Total Phenolic and Total Flavonoid
Contents of Tainong 73
Treatment1
Flesh
Fresh samples
Sun-drying
LTD at 25C
HAD at 50C
HAD at 75C
Total Phenolics2
A3
Total Flavonoids2
B3
243.020.32a
23.210.10c
27.620.01c
76.110.02b
76.100.08b
90.95
88.63
68.68
68.68
42.250.09a
19.620.07e
21.780.17d
34.450.02c
36.200.02b
53.56
48.45
18.46
14.32
Fresh samples
258.950.67a
104.250.12a
Sun-drying
74.160.05e
71.36
57.480.06e
44.86
LTD at 25C
94.810.28d
63.39
61.780.14d
40.74
HAD at 50C
185.0324.35c
28.55
70.540.12c
32.34
HAD at 75C
237.481.11b
8.29
99.810.02b
4.26
Skin
LTD: low-temperature drying; HAD: hot air drying; means with a same letter in a column are not
significantly different (p>0.05).
A and B are extent of decrease (%) of total phenolics and total flavonoids respectively, during the drying
process, with regard to the fresh samples values.
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Florida,
USA:
Brooks/Cole
Publishing Company.
Larraruri, J. A., C. Sanchez-Moreno, and
F. Saura-Calixto. 1998. Effect of
Temperature on the Free Radical
Scavenging Capacity of Extracts
from Red and White Grape Pomace
Peels. Journal of Agricultural and
Food Chemistry 46: 2694-2697.
Marc,
F.,
A.
Davin,
L.
Deglne-Benbrahim, C. Ferrand, M.
Baccaunaud, and P. Fritsch. 2004.
Mthodes dvaluation du Potentiel
Antioxydant
Dans
les
Aliments. Medecine Sciences 20:
458-63.
Marinova, D., F. Ribarova, and M.
Atanassova. 2005. Total Phenolics
and Total Flavonoids in Bulgarian
Fruits and Vegetables. Journal of
the University of Chemistry and
Technological Metallurgy 40 (3):
255-260.
Miliauskas, G, P. R. Venskutonis, and T.
A. van Beek. 2004. Screening of
Radical Scavenging Activity of
Some Medicinal and Aromatic Plant
Extracts. Food Chemistry 85:
231-237.
Molyneux, P. 2004. The Use of the
Stable
Free
Radical
diphenylpicrylhydrazyl (DPPH) for
Estimating Antioxidant Activity.
Songklanakarin Journal of Science
and Technology 26(2): 211-219.
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