Professional Documents
Culture Documents
of
Technology and Medicine
Malabe
Sri Lanka
Faculty of Medicine
HANDBOOK
FOR STUDENTS
IN
MEDICAL MICROBIOLOGY
and
IMMUNOLOGY
2014
CONTENTS
1. Introduction
2. The staff in the department
3. The time table
4. The course content and topics
5. General objectives of the course
6. Specific objectives in
6.1 General microbiology
6.2 immunology
6.3 systematic bacteriology
6.4 virology
6.5 mycology
6.6 antibiotics
7. The teaching learning methods
7.1 Lectures
7.2 Practical sessions and demonstrations
7.3 Small group discussions
7.4 Tutorials
8. Assessments
8.1 Continuous summative and formative assessments
8.1.1 Control tests
8.1.2 Credit tests
8.2 Final assessment in microbiology and immunology
9. Recommended reading
APPENDICES
1. Gram Stain
2. Acid Fast stain
3. Gram stain assessment
1. Introduction
Welcome to the Department of Microbiology and to the course in
Microbiology.
Microbiology is the study of microscopic organisms. This course deals with
all aspects of microorganisms (bacteria, viruses and fungi), that cause
disease in humans, and those that have the potential to cause disease.
The learning of Microbiology should go hand in hand with your clinical
work in the wards. You should be able to relate the knowledge you learn in
the subject to cases of infectious diseases that you see and discuss in the
wards. In this way the subject becomes more interesting and relevant to
your ward work and also when you complete this course and move on to
become a medical doctor.
2. The staff in the department
Academic staff
Professors/ Senior Lecturers:
Nelun de Silva MBBS (Patna), Dip Micro(Col), MD Micro (Col)
Anura Weerasinghe -MBBS, MD, FRCP, DCH, DTM&H, FCCP, PhD
Nalaka Kanakaratne MD (Rus) MSc. (Kelaniya) PhD (Pera)
Lecturers:
Dr. Vindya Perera BVSc (Peradeniya)
Ms. J. Lohitharajah -BSc (Human Biology)(Hon) on study leave
3.
At a glance the hours that are set aside for Microbiology in a week are as
follows:
SEMESTER 1V
6hours
Lectures in Microbiology and Immunology
2 hours
Small group discussions
Hands on Practical sessions
Practical demonstrations
Tutorials, Case studies
SEMESTER V
2 hours
Lectures in Microbiology - on line and face
to face
2 hours
Small group discussions
Practical demonstrations
Tutorials, Case studies
4.
6.
Specific objectives
6.1 SPECIFIC OBJECTIVES IN GENERAL MICROBIOLOGY
At the end of each session of teaching learning on general
microbiology, the students should be able to:
1. List the generic names of common microorganisms (Bacteria,
Viruses & Fungi), which cause disease in humans.
2. Recognize the basic microscopic features of the common
bacterial pathogens and Fungi and identify them in direct mounts,
Gram and acid fast smears.
3. Select the microbes that are encountered in this country and
prioritize them in order of importance.
3.1 Describe the habitat and routes of transmission and explain
the pathogenesis of infections caused by these.
3.2 Relate the clinical signs and symptoms of these diseases to
the underlying pathogenesis.
3.3 List microbiological tests available for the diagnosis of these
infectious diseases.
3.4 Select appropriate tests according to the duration of the
illness.
3.5 Advise on and be able to collect and transport relevant
microbiological specimens.
3.6 Select appropriate antimicrobial agents that can be used in
treatment and prophylaxis, being aware of
the guidelines for empiric therapy, culture and antibiotic
susceptibility test (ABST) reports and limitations( cost, availability,
patient factors, etc..)
3.7 Explain the measures for the prevention and control of
these diseases.
4. Explain the mechanism of action, identify the spectrum of
activity of antimicrobial agents and describe the resistance
mechanisms of microorganisms against antimicrobials.
5. Advise on appropriate immunization procedures for the bacterial
and viral diseases.
6. Define and list the antiseptics, disinfectants and sterilizing
agents, explain their mode of action and select the appropriate
ones for patient care and in the laboratory.
7. Describe the normal and exaggerated immunological responses
of a human host when exposed to a pathogen / foreign antigen/
self antigens.
10
1.
11
12
13
14
30
20
20
10
20
100
Students obtaining less than 50% in the total mark has to repeat
all components of the examination
To be eligible for distinction a student should obtain at least 75%
in the total mark and performed equally well in all components of
the examination.
Compilation of the final mark as a percentage Repeat examination
SEQ/SAQ
MCQ
OSPE
Viva voce
Total
40
25
25
10
100
9. Recommended Reading
1. Medical microbiology by D. Greenwood, R. Slack, J. Peutherer.-Churchill
Livingstone.
2. Medical Microbiology by Brooks G.F.
et al. Jawetz, Melnick,
st
Adelberg's . 21 ed. Appleton & Lange, 1998.
3. Medical Microbiology by Mims C. A., Playfair J. H. L., Roitt I., Wakelin
D., Williams R., Anderson R. M... Mosby. 1993.
th
4. Immunology by Roitt I., Brostoff J., Male D. 5 ed. Mosby. 1998.
5. Basic immunology by Sharon J.. Williams & Wilkins. 1998.
th
6. Immunology by Weir D. M., J. S. Churchill Livingston. 8 ed. 1997.
APPENDICES
APPENDIX 1: PRESTON & MORELL'S MODIFICATION OF GRAM'S STAIN
1. Apply Ammonium Oxalate-Crystal Violet stain for 1/2 minute.
2. Wash thoroughly with water.
3. Apply Lugol's Iodine solution for 1/2 minute.
4. Wash thoroughly with water.
5. Apply Iodine-Acetone solution for 1/2 minute.
6. Wash thoroughly with water.
7. Counter stain with dilute Carbol Fuchsin for 1/2 minute.
8. Wash thoroughly with water.
The whole smear must be flooded with each reagent and the previous
reagent must be completely washed at each stage. Care must also be taken
not to waste stains by over flooding the slide with the stain.
15
16