2015 AAPS Annual Meeting and Exposition

29/10/15 08:50

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Sessions: Tuesday Contributed Paper Posters
T3227 - Oxidative Stability of Bullfrog (Rana catesbeiana Shaw) Oil

University of Iowa
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Drug Delivery-Technol-SMFormulation
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Sessions: Tuesday Contributed Paper Posters

See attached abstract pdf for images.

Authors: Renata Rutckeviski, Federal University of Rio Grande do Norte (Main Author); Lucas Machado, Federal University of Rio Grande do Norte;
Everton Alencar, University of Iowa (Presenting Author); Andreza Morais, Federal University of Rio Grande do Norte; Francisco Xavier, Jr., Federal
University of Rio Grande do Norte; Aleph Souza, Federal University of Rio Grande do Norte; Wogenes Oliveira, Federal University of Rio Grande do
Norte; Franceline Reynaud, Federal University of Rio Grande do Norte; Eryvaldo Egito, Federal University of Rio Grande do Norte
Presenting Author: Everton Alencar
Purpose: To evaluate the oxidative stability of bullfrog (Rana catesbeiana Shaw) oil.
Methods: The oxidative stability of pure bullfrog oil (BO), BO with butylated hydroxyanisole (BHA) (0.02% and 0.5% w/w) and butylated
hydroxytoluene (BHT) (0.01% and 0.5% w/w) were monitored by the peroxide value (PV), the acidity value (AV), the iodine value (IV) and the
saponification value (SV). The stability was evaluated by the Schaal oven test, in which samples were stored at 60 2 C for 15 days. Dienes (DC) and
trienes (TC) conjugated were determined by UVVis Spectrophotometer, after hexane dilution (10 mL). The absorption at 232 and 273 nm were used to
identification of DC and TC, respectively. The oxidation induction time (OIT) was evaluated by the Rancimat method, in which 3 g of each sample were
heated at 110 1C, under air flow of 10 L.h-1. The volatile compounds produced during the oxidation process were captured and analyzed into a
conductivimetric cell containing water.
Results: After 15 days of storage the PV and SV increased significantly for all samples after the heating process. However, the addition of antioxidants,
mainly BHT at 0.5 % reduced thirteen times the PV and the SV compared to the BO itself. The AV on the BO sample increased 3.2 times in 15 days of
storage (9.33 mg of potassium hydroxide (KOH).g-1 oil). However, the addition of BHA (0.02 %) and BHT (0.01 %) maintained low levels until the
twelfth day. The IV of the BO sample showed a reduction of 43 % after 15 days under the Schaal oven test. On the other hand, the addition of both BHT
(0.01 %) and BHA (0.02 %) induced a reduction of 33 % in this value. The BO showed a DC of 6.5 g.mL-1 0.16, which may be related to the presence
of initial peroxides. However, the addition of BHA at 0.02 % and 0.5 % reduced to 22 % and 68 % the DC concentration, respectively. The addition of
BHT at 0.01 % and 0.5 % reduced the DC value at 40 % and 83 %, respectively. The TC increased significantly after 15 days of storage. This may be due
to the peroxides instability under heating, leading to secondary oxidation products that absorb at 273 nm. Samples showed a significant TC reduction with
the addition of BHT at both concentrations. The BO showed an OIT of 0.32 0.01 h and the addition of BHA at 0.02 % or 0.5 %, decreased this value.

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2015 AAPS Annual Meeting and Exposition

29/10/15 08:50

Furthermore, the addition of BHT (0.01 %) increased the OIT two times, while the addition of the BHT at 0.5 % increased the OIT 24 times (7.88 h) (p <
0.05).
Conclusion: The oxidative stability of the BO can be improved with the antioxidants addition. BHT has proven to be more effective than BHA in
increasing the oxidative stability of the BO, allowing the oil to be used in the development of new pharmaceutical products for wound treatment, asthma
and inhibition of biofilm formation.
Abstract Link: http://abstracts.aaps.org/Verify/AAPS2015/PosterSubmissions/T3227.pdf
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Contributed Poster
Tuesday Afternoon Poster Session

1:30 PM5:00 PM Oct 27, 2015

OCCC - Exhibit Hall WB1-WB2
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