BIOL 350 Exam A Fall 2010

BIOL 350: General Microbiology

Exam 1

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BIOL 350 Exam A Fall 2010

1.WhichtypeofmicroscopywouldbethebesttovisualizeabacterialSlayer?
A. fluorescentmicroscopy.
B. lightmicroscopy.
C. scanningelectronmicroscopy.
D. transmissionelectronmicroscopy.
E. bothBandD.
2.WhichstructureaccountsforwhyGrampositiveandGramnegativebacteriastaindifferently
whenusingtheGramstain?
A. periplasmicspace
B. LPS
C. Slayer
D. peptidoglycan
E. alloftheabove
3. Whichcondition(s)favorlyticgrowthbylambdabacteriophage?
A. HighlevelsofcIrepressor
B. LowMOI.
C. HighMOI.
D. Bacteriainstationaryphase.
E. BandDonly.
4. Whenperformingfluorescentmicroscopy,whichstainassociatesexclusivelywithDNA?
A. DAPI.
B. GFP.
C. ethidiumbromide.
D. FITC.
E. alloftheabove.
5. DuringtheprocessofChemotaxis,Attractantsresultinwhichofthefollowing?
A. Counterclockwiserotationoftheflagella.
B. Tumblingofflagella.
C. Arandomwalk.
D. Clockwiserotationofflagellatopushthecellforward.
E. bothAandC.

BIOL 350 Exam A Fall 2010

6.Staphylococcusiswhichofthefollowing?
A. AGramnegativecocci.
B. AGrampositivebacilli.
C. AGrampositivevibrio.
D. AGramnegativebacilli.
E. AGrampositivecocci.
7. MacConkeyagarcontains________thatisusedtoselectforthegrowthof________organisms?
A.Lactose,Grampositive
B. Bilesalts,Grampositive
C. apHindicater,Gramnegative
D. Bilesalts,Gramnegative
E. Lactose,Acidophiles
8. Glycerolistypicallybroughtintothecellbywhichprocess?
A. ABCtransporters
B. PTSsystem
C. Antiport
D. Symport
E. FacilitatedDiffusion
9. WhichofthefollowinginNOTacharacteristicofavirus.
A. assemblecapsids.
B. attachtohost.
C. replicategenome.
D. growanddivide.
E. releaseprogenyvirusesfromthehostcell.
10. WhichofthefollowingcellsurfacestructurescanbefoundonGrampositivebacteria?
A. capsule
B. Slayer
C. pili
D. flagella
E. alloftheabove

BIOL 350 Exam A Fall 2010

11.Peptidoglycanconsistsoftwojoinedaminosugars(NAMandNAG):duringcellwallsynthesis
whichenzymesareresponsibleforformingthecrosslinksbetweentherowsandlayersof
peptidoglycan?
A. autolysins.
B. transpeptidases.
C. transglycosidases.
D. bactoprenols.
E. Nacetylglucosamines.
12. Ahalophilicorganismrequireswhichofthefollowingcondition(s)togrow?
A. apHbetween911.
B. ahighlevelofoxygen.
C. highconcentrationofNaCl.
D. atemperatureof0oC20oC.
E. cangrowatbothhighandlowlevelsofoxygen.
13. Pilifoundonthesurfaceofbacteriaactasa(n)____________?
A. specializedphospholipid.
B. endotoxin.
C. adhesin
D. corepolysaccharide.
E. alloftheabove
14. WhichofthefollowingisonlyfoundinGramnegativebacteria?
A. capsule
B. LPS
C. Slayers
D. peptidoglycan
E. flagella
15. WhichofthefollowingstatementsisFALSEabouttheprocessofcelldivision?ALL
ANSWERSACCEPTABLE
A. DNAreplicationisbidirectional.
B. Cellundergoesseptationattheequator.
C. Cellelongatesandaddsanewwallatthecellequator.
D. Cellcanbeginanewroundofreplicationoncethecellhasdivided
E. allstatementsaretrue.

BIOL 350 Exam A Fall 2010

16. WhichofthefollowingisTRUEregardingregulationofthelacoperonwhenLactoseispresent
(andglucoseisabsent)
A. theCRPactivatorproteincannotbindthelacpromoter.
B. theLacIrepressordoesnotbindtotheLacoperator.
C. cAMPlevelsarelow.
D. allolactosebindstotheLacpromoter.
E. Alloftheabovearetrue.
17. Bacteriaproduce2offspringpergeneration,butifanewlyidentifiedorganismfromMars
producesnineoffspringpergeneration,whichofthefollowingwouldexpresstherateof
increaseinpopulation,wherencorrespondstothenumberofgenerations?
A. 2n
B. n2
C. 9n
D. n9
E. 2n9
18. Whennutrientsarelimitedwhatisthemostlikelygrowthphaseabacterialculturewillbein?
A. lagphase
B. logphase
C. deathphase
D. stationaryphase
E. bothAandB
19. IfabacteriumobtainsCarbonfromanoutsidesourceandrequiresinorganiccompoundsas
sourcesofenergy/electrons,itwillbereferredtoasa/an______?
A. Photoautotroph
B. Photoheterotroph
C. Chemoheterotroph
D. Chemoautotroph
E. Autotroph
20. Taqpolymerase,whichisusedinPCR,isisolatedfromwhichtypeoforganism?
A. halophiles.
B. psychrophiles.
C. mesophiles.
D. thermophiles.
E. barophiles.

BIOL 350 Exam A Fall 2010

21. Whichbacteriamakeadormantformcalledanendosporethatisresistanttodesiccationand
othernaturalstresses?
A.Bacillusanthracis.
B. Bacilluscereus.
C. Clostridiumtetani.
D. Clostridiumbotulinum.
E. alloftheabove.
22. WhatistrueofaSensorKinaseinaTwoComponentSignalTransductionSystem?BOTHB
ANDDACCEPTED
A. Bindsthechromosometoaltertranscription.
B. Activatesitselfviaphosphorylation.
C. Bindstotheresponseregulator.
D. Foundintheplasmamembrane.
E. alloftheabove.
23. Theperiodoftimewhenvirionsareassemblinginsidetheinfectedcellisthe?
A. eclipseperiod.
B. latentperiod.
C. riseperiod.
D. burstperiod.
E. lagperiod.
24. Aprophagereferstowhatpartofthebacteriophagelifecycle?
A. Theprocessofreleaseofinfectiousparticles.
B. Wheretheviralgenomeisintegratedintothehostchromosome.
C. Whenalysogenisinducedtoenterlyticcycle.
D. Theinjectionoftheviralgenomeintothehostcell.
E. Theassemblyofearlyphageparticlesinsidethehostcell.
25. Abacterialphagethatiscapableofalyticrelationshipwiththeirhostiscalledwhattypeof
phage?
A. Virulent
B. Prophage
C. Lysogenic
D. Temperate
E. Inductant

BIOL 350 Exam A Fall 2010

26.Amissensemutationmaygenerate:
A. aframshift.
B. asilentmutation.
C. astopcodonmutation.
D. alloftheabove
E. BandConly
27. WhatisTRUEaboutamicroaerophilicbacterium?
A. willgrowinhighlevelsofoxygen.
B. cantoleratebothhighandlowlevelsofoxygen.
C. isananaerobe.
D. willgrowinlowlevelsofoxygen.
E. noneoftheabovearetrue.
28. AnFplasmidthathasintegratedintothehostgenomeiscalleda/an?
A. Hstrain
B. F+strain
C. Fstrain
D. F'strain
E. Hfrstrain
29. Genetransferthatinvolvesalyticbacteriophageisknownas?
A. conjugation.
B. specializedtransduction.
C. generalizedtransduction.
D. transformation.
E. bothBandC.
30. WhatdidtheUTubeexperimentdemonstrate?
A. specializedtransductionrequiresthelyticpathway.
B. conjugationonlyoccursinauxotrophs.
C. transformationrequiresuptakeofnakedDNA.
D. generaltransductionoccursviabacteriophage.
E. conjugationrequirescelltocellcontact.

BIOL 350 Exam A Fall 2010

31. WhathappensduringconjugationbetweenanHfrstrainandFstrain?
A. TheFstrainbecomesF+.
B. PortionsofthebacterialchromosomearetransferredtotheFstrain.
C. TheHfrstraintransferstheFplasmid.
D. alloftheabove
E. noneoftheabove
32.WhichofthefollowingisNOTtrueofDNAreplicationinE.coli?
A. Itissemiconservative.
B. Itisbidirectional.
C. Thesecondstrandissynthesizeddiscontinuously.
D. Involvesokazakifragmentsinthelaggingstrand.
E. Synthesisoccursinthe3'to5'direction.
33. WhichmoleculeisresponsibleforloadingthehelicaseduringDNAreplication?
A. DNAprimase
B. DnaC
C. DNApolymeraseIII
D. DnaA
E. DnaB
34. Ifabacteriumcangrowonadefinedminimalmedia,itiscalleda(n)________?
A. prototroph
B. autotroph
C. heterotroph
D. auxotroph
E. chemotroph
35. ProtonMotiveForceisusedtodrivewhichprocess?
A. RotationofATPsynthase.
B. Drugeffluxpumps.
C. flagellarmotility.
D. Symporttransport.
E. alloftheabove.

BIOL 350 Exam A Fall 2010

36.DnaBisresponsibleforwhichprocessduringDNAreplication?
A. attachingRNAprimertosinglestrandedDNA.
B. loadingthehelicase.
C. removingtheRNAprimer.
D. unwindingthedoublestrandedDNAhelix.
E. bindingtheorigintoinitiateDNAopening.
37. Ifageneisonlyexpressedundercertainconditions,itisconsideredtobe?
A. constitutive.
B. inducible.
C. alwaysexpressed.
D. silent.
E. anonsensemutation.
38. Virusescanbeclassifiedaswhichdomainoflife?
A. bacteria
B. archaea
C. acytota
D. eukarya
E.none,itisnotalivingcell
39. WhichofthefollowingisTRUEregardingregulationoftheLacoperonwhenLactoseis
absent?
A. TheLacIrepressorfallsofftheoperon.
B. LacZistranscribed.
C. allolactosebindstothepromoter
D. LacIisboundtothelacoperator.
E. alloftheabovearetrue.
40. WhathappensinthecellwhenGlucoseispresent?
A. CRPisactiveandbindstolacpromoter.
B. adenylatecyclasebecomesactive.
C. lacoperonisnottranscribed.
D. cAMPismadeathighlevels.
E. alloftheabove.