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Acta Biomaterialia
journal homepage: www.elsevier.com/locate/actabiomat
Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
Department of Mechanical Engineering, Ege University, 35100 Bornova, Izmir, Turkey
c
Advanced Material Research and Development Center, Zhejiang Industry & Trade Vocational College, Wenzhou, Zhejiang 325003, China
d
Department of Materials Science and Engineering, Jilin University, Changchun, Jilin 130025, China
e
School of Aerospace, Mechanical and Manufacturing Engineering, RMIT University, Melbourne, Victoria 3083, Australia
b
a r t i c l e
i n f o
Article history:
Received 13 September 2014
Received in revised form 19 March 2015
Accepted 20 March 2015
Available online 25 March 2015
Keywords:
Elastic admissible strain
Cytocompatibility
TNZ (TiNbZr) alloys
Mechanical properties
Youngs modulus
a b s t r a c t
A new series of beta TiNbZr (TNZ) alloys with considerable plastic deformation ability during
compression test, high elastic admissible strain, and excellent cytocompatibility have been developed
for removable bone tissue implant applications. TNZ alloys with nominal compositions of Ti34Nb
25Zr, Ti30Nb32Zr, Ti28Nb35.4Zr and Ti24.8Nb40.7Zr (wt.% hereafter) were fabricated using the
cold-crucible levitation technique, and the effects of alloying element content on their microstructures,
mechanical properties (tensile strength, yield strength, compressive yield strength, Youngs modulus,
elastic energy, toughness, and micro-hardness), and cytocompatibilities were investigated and compared.
Microstructural examinations revealed that the TNZ alloys consisted of b phase. The alloy samples displayed excellent ductility with no cracking, or fracturing during compression tests. Their tensile strength,
Youngs modulus, elongation at rupture, and elastic admissible strain were measured in the ranges of
704839 MPa, 6265 GPa, 9.914.8% and 1.081.31%, respectively. The tensile strength, Youngs modulus
and elongation at rupture of the Ti34Nb25Zr alloy were measured as 839 31.8 MPa, 62 3.6 GPa, and
14.8 1.6%, respectively; this alloy exhibited the elastic admissible strain of approximately 1.31%.
Cytocompatibility tests indicated that the cell viability ratios (CVR) of the alloys are greater than those
of the control group; thus the TNZ alloys possess excellent cytocompatibility.
2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
1. Introduction
According to a report by the United Nations in 2013 [1], the
population of the age group 60 years and older is increasing
rapidly, and in the more developed regions of the world, the ratio
of elderly people is expected to increase by 45% as of 2050.
Orthopedic biomaterials are implanted into human bodies in order
to heal bone tissue diseases developed due to aging, various congenital defects in bone tissue, injuries to bone tissue and joints
from trafc and sports accidents [2,3].
With the increasing utilization of orthopedic implant materials,
if biologically and mechanically compatible materials are not used
for implants, it is inevitable that the number of revision surgeries
will increase [4]. Genotoxic, cytotoxic, carcinogenic, mutagenic,
allergenic, neurological effects are taken into account while
Corresponding author at: School of Aerospace, Mechanical and Manufacturing
Engineering, RMIT University, Melbourne, Victoria 3083, Australia. Tel.: +61 3 9925
7290.
E-mail address: cuie.wen@rmit.edu.au (C. Wen).
http://dx.doi.org/10.1016/j.actbio.2015.03.023
1742-7061/ 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
177
Bo RX i Bo i
Md RX i M d i
where Xi is the atomic ratio of the given element, (Bo)i is the bond
order value of the given element [54], and (Md)i is the metal
178
179
Fig. 2. Optical micrographs of as-cast TNZ alloys: (a) TNZ-1; (b) TNZ-2; (c) TNZ-3; (d) TNZ-4.
180
Fig. 4. Microhardness of TNZ alloys in comparison with those of other Ti alloys published in the literature.
181
182
Fig. 8. Tensile mechanical properties (tensile strength, yield strength and elongation at rupture) of TNZ alloys.
of the toughness and elastic energy of the alloys from the highest
to lowest is TNZ-1 > TNZ-2 > TNZ-3 > TNZ-4. As seen in Fig. 9, the
average Youngs modulus of the alloys was in the range of
6265 GPa.
Ti alloys that are used as implant material are required to possess high mechanical strength and a low Youngs modulus, so that
they can safeguard the host bone tissue without causing stress
d r=E
183
Fig. 9. Tensile mechanical properties (Youngs modulus, elastic energy and toughness) of TNZ alloys.
Table 1
Comparison of mechanical properties of TNZ alloys with those of some commercial titanium alloys.
Ti alloys
Yield strength
(MPa)
Tensile strength
(MPa)
Youngs
modulus (GPa)
Elongation (%)
Elastic admissible
Strain (%)
Alloy type
References
Pure Ti (Grade 4)
Ti16Nb10Zr (annealed)
Ti15Mo (annealed)
Ti-6-4 ELI (mill annealed)
Tiadyne 1610 (aged)
Ti35.3Nb5.1Ta7.1Zr (annealed)
Ti29Nb13Ta4.6Zr (annealed)
CP-Ti (cold worked)
Ti34Nb25Zr (TNZ-1)
Ti32Zr30Nb (TNZ-2)
Ti35.4Zr28Nb (TNZ-3)
Ti40.7Zr24.8Nb (TNZ-4)
485
485
544
875
736
547
607 10.3
810 48.0
782 18.8
729 26.3
682 52.9
550
520
874
965
851
597
549
758 14.1
839 31.8
794 10.1
755 28.3
704 49.5
104.1
70
78
110
81
55
60
109 1.1
62 3.6
65 4.2
64 4.5
63 4.2
15
22
21
1015
I0
19
41.6
26 0.2
14.8 1.6
13 1.8
11.3 1.5
9.9 1.1
0.47
0.69
0.70
0.80
0.91
0.99
0.56
1.31
1.20
1.14
1.08
[8]
[83]
[84]
[84]
[84]
[8]
[81]
[This
[This
[This
[This
[This
b + a
b
a+b
b
b
b
a
b
b
b
b
study]
study]
study]
study]
study]
184
Fig. 10. Fracture surfaces of as-cast TNZ alloys (a) TNZ-1; (b) TNZ-2; (c) TNZ-3; (d) TNZ-4.
Fig. 11. Morphology of SaOS2 cells attached on the surface of TNZ-2 alloy after cell
culture for 24 h.
185
Fig. 12. SEM images of SaOS2 cells attached on to surfaces of TNZ alloys after cell culture for 7 days: (a) TNZ-1; (b) TNZ-2; (c) TNZ-3; (d) TNZ-4.
Fig. 13. SaOS2 cell adhesion density of as-cast TNZ alloys and CP-Ti after cell culture for 7 days.
4. Conclusions
The microstructures, mechanical properties and cytocompatibility of the as-cast TiNbZr alloys have been examined. The conclusions are as follows:
1. Microstructural examinations revealed that the TNZ alloys display primary grain boundaries together with secondary grain
structure.
2. The average compressive yield strength and microhardness
values of the TNZ alloys exhibited the same ranking: TNZ2 > TNZ-3 > TNZ-1 > TNZ-4. The alloys exhibited considerable
186
Fig. 14. Cell viability ratio of as-cast TNZ alloys and CP-Ti.
Acknowledgments
SO would like to acknowledge the Council of Higher Education
(CoHE) of Turkey for PhD research scholarship. CW acknowledges
the nancial support from the Australian Research Council (ARC)
through the ARC Discovery Project DP110101974.
Appendix A. Figures with essential color discrimination
Certain gures in this article, particularly Figs. 5 and 79 are difcult to interpret in black and white. The full color images can be
found in the on-line version, at http://dx.doi.org/10.1016/j.actbio.
2015.03.023.
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