LESSON PLAN

CBE 451 BIOCHEMISTRY AND

METABOLIC REGULATION

FACULTY OF CHEMICAL ENGINEERING

UNIVERSITI TEKNOLOGI MARA
PAGE

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1.0

COURSE INFORMATION
Course Name
: Biochemistry and Metabolic Regulation
Code
: CBE 451
Programme
: EH 222
Semester
:2
Credit Hours
:3
Contact Hours
:4
Course Status
: Core
Prerequisite
: NIL

2.0

SYNOPSIS
This course imparts the fundamental knowledge needed for bioprocess engineering in
terms of chemical aspects of life from molecular point of view. The course provides
the necessary knowledge of protein structure and function, metabolic pathways and
molecular biology. It also includes recent advances in biotechnology, and
technological implications of biochemical engineering in life sciences.

3.0

OBJECTIVES
Upon completion of this course, students should be able to:
1. Explain the basic structure of biomolecules in relation to their functional groups.
2. Explain various metabolic pathways including glycolysis and TCA cycle and
explain how they are regulated in the cells.
3. Understand the techniques, skills, and modern engineering tools necessary for
engineering practice.

4.0

METHODS OF INSTRUCTION
Lectures and tutorials

5.0

LESSON PLAN

WEEK
1

CHAPTER / CONTENT
Chapter 1
Molecular Components of Cells
1.1 Importance of water; Structure and function of
biomolecules: Carbohydrates, Lipids, Proteins and
Nucleic acids.

1.2 Biomolecules and their conformation;

Ramachandran map; Weak inter-molecular
interactions in biomacromolecules;
1.3 Protein structure, folding and function: Myoglobin,
Hemoglobin, Lysozyme, Ribonuclease A,
Carboxypeptidase and Chymotrypsin.
1.4

Biological membranes; Transport across

membranes and pumps; Signal transduction;

ACTIVITIES
Introduce and explain lesson
plan and course outline.
Describe the properties of
water, the biological solvent.
Introduce structure and function
of biomolecules
Describe the structures, classes,
properties, functions proteins.

Describe chemical and physical

properties
of
biological
membranes.
Introduce nucleotides, nucleic

LESSON PLAN
CBE 451 BIOCHEMISTRY AND
METABOLIC REGULATION

FACULTY OF CHEMICAL ENGINEERING

UNIVERSITI TEKNOLOGI MARA
PAGE

2/4
acids, genetic information.

hormones and neurotransmitters.

1.5 Nucleotides and Nucleic Acids: Structure and

chemistry of nitrogeneous bases, nucleotides, and
classes of nucleic acids.
1.6 Thermodynamics of Biological Systems: Basic
concepts of thermodynamics, effect of
concentration on net free energy changes, effect
of pH on standard-state free energies,
relationships between thermodynamic parameters
and biochemical events.
Chapter 2
Protein Dynamics

Explain and discuss biological

order, laws of thermodynamics,
free energy changes.
Read page 92-101 of McKee
Read page 161-188 of McKee

2.1 Chemical and functional nature of enzymes;

Kinetics of single substrate and bi-substrate
enzyme catalysed reactions; inhibition of enzyme
activity; Bioenergetics.
5

6-7

2.2 Enzyme kinetics including its regulation and

inhibition, and Coenzymes.
Chapter 3
Metabolism and Its Regulation
3.1 Metabolism: An Overview

Read page 189-193 of McKee

TEST 1
Describe the metabolic maps,
anabolic and catabolic
processes, how to elucidate
metabolic pathways.
Read page 234-245 of McKee

3.2 Metabolism and bioenergetics; Generation and

utilisation of ATP.
9-10

Read page 272-289 of McKee

Read page 298-324 of McKee

3.3

11-12

Metabolic pathways and their regulation:

glycolysis, TCA cycle, pentose phosphate pathway,
oxidative
phosphorylation,
gluconeogenesis,
glycogen and fatty acid metabolism.
Chapter 4
Nucleic Acids - Information Transfer
4.1 DNA replication, transcription and translation;
Biochemical regulation of gene expression.

Read Chapter 17, 18 of McKee

TEST 2

4.2 Recombinant DNA technology and applications:

PCR, site directed mutagenesis and DNAmicroarray.

13-14

Chapter 5

Discussion of biochemical
techniques for macromolecular

LESSON PLAN

FACULTY OF CHEMICAL ENGINEERING

UNIVERSITI TEKNOLOGI MARA

CBE 451 BIOCHEMISTRY AND

METABOLIC REGULATION

PAGE

3/4
analysis

Techniques
5.1 Biochemical and biophysical techniques for
macromolecular analysis;
5.2

Biochemical
separation
techniques
and
characterisation: ion exchange, size exclusion and
affinity chromatography, electrophoresis, UVvisible, fluorescence and Mass spectrometry.

Chapter 6
Special Topic: Bioinformatics

6.0

7.0

ASSESSMENTS
Assignment
Tests
Examination

: 20%
: 20 %
: 60 %

GRADING SCALE
A+
A
AB+
B
BC+

90 - 100
80 - 89
75 - 79
70 - 74
65 - 69
60 - 64
55 - 59

C
CD+
D
E
F

50 - 54
47 - 49
44 - 46
40 - 43
30 -39
0 - 29

8.0

REFERENCES
McKee T.M., McKee J.R., Biochemistry. The Molecular Basis of Life, 3rd Edition,
McGraw Hill, 2003.

9.0

ADDITIONAL INFORMATION
Please make appointments for consultancy.
Attendance is compulsory.
No make up test will be given unless with medical reasons with legal documents
presented.
No cheating in any aspect. Non-compliance will be met with severest disciplinary
actions.

PREPARED BY:
SIGNATURE:

APPROVED BY:
SIGNATURE:

LESSON PLAN
CBE 451 BIOCHEMISTRY AND
METABOLIC REGULATION
NAME: DR NIK RAIKHAN NIK HIM
DATE: 8 SEPTEMBER 2014

FACULTY OF CHEMICAL ENGINEERING

UNIVERSITI TEKNOLOGI MARA
PAGE

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NAME: DR FAZLENA HAMZAH

POSITION: KPP Bio-Chemical Engineering
DATE: 8 SEPTEMBER 2014