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-Enumeration of total
microorganisms in a sample
using fluorescent stains
-Enumeration of viable cells in a
sample using viable stains
-Identification of microorganisms
using genetic stains
10/28/13
Methods in Microbial Ecology
PART 2
However,
When high sensitivity is required (e.g., low levels of particular molecule)
Turnover rates need to be determined
Fate of portions of a molecule need to be followed
Radioisotopes (e.g., 35S, 14C) are generally used
How can microbial activity be distinguished from
abiotic processes?
A killed cell control is required to prove that the
transformations are not due to abiotic processes
10/29/2013
10/29/2013
Apprill 2007 Coral Reefs Visibly healthy corals exhibit variable pigment concentrations
and symbiont phenotypes, 26:387397
Microbial community
14C- organic compound for chemoorganotrophs
(or 14C- CO2 for autotrophs) and incubation
MAR- FISH
Combines the identification and activity measurements of a microbial community
Fig (a) a Proteobacteria (FISH analysis) and an autotroph (MAR- measured uptake of 14CO2)
Fig (b) uptake 14C-glucose by a mixed culture of E. coli (yellow cells FISH analysis) and Herpetosiphon
aurantiacus (filamentous green cells)
Fig (c) MAR of the same field of cells shown in b 14C-glucose assimilated by E. coli only