Professional Documents
Culture Documents
I. Mutual Characteristics: Both genera are Gram positive and have the
similar spherical cell shape, therefore there are called cocci (in Greek coccus
means granule).
II. Visual differentiation between Staphylococci and
Streptococci by cellular arrangement:
When performing laboratory analysis, after Gram staining, first thing we
should do is put a piece of a colony under microscope and observe cellular
arrangement of the bacteria. Both staphylococci and streptococci have round,
spherical cell shape, but the arrangement of cells is different due to a
different binary fission. Streptococci form a chain of round cells, because
their division occurs in one linear direction, whereas staphylococci divide in
various directions forming grape-like clusters.
Catalase test
Coagulase test
Staphyloslide
test
Colour
differences in
colonies
Hemolysis test
Reaction to
Novobiocin
Staphylococcus
aureus
Staphylococcu
s epidermidis
+
+
+
+
-
Staphylococc
us
saprophyticu
s
+
-
Gold yellow
White
White to yellow
Beta
Sensitive
None
Sensitive
None
Resistant
S. pyogenes
S. agalactiae
Lancefield
group
Hemolysis
Diagnostic
features
A
B
Beta
Beta
Bacitracin sensitive
Bacitracin resistant
Hippurate
hydrolyzed
S. faecalis
(Enterococcus)
S. bovis
D
D
Alpha or Beta or
none
Alpha or none
S. pneumoniae
Not applicable
Alpha
Viridans group
Not applicable
Alpha
Growth in 6.5%
NaCl
No Growth in 6.5%
NaCl
Bile soluble
Inhibited by
optochin
Not bile soluble
Not inhibited by
optochin
Catalase test
Positive (exception: Staphylococcus
aureus, subspecies anaerobius is
catalase negative)
Negative
Negative
Negative
Negative
Positive
Rare types from moderate to mostly
non-reactive
Moderate positive reaction
Mostly non-reactive
Negative