MCB 3020 Laboratory Schedule Spring 2015

TA office: HPA II 304

MCB 3020 GTA: Bracken Roberts | bracken.roberts@knights.ucf.edu

Date

Day

Title

Lab Safety, Introduction to Lab Equipment, and

Aseptic Technique
Buy Lab Coat, Lab Manual, Packet from Book Store
Introduction to Brightfield Microscopy
Wetmount
1/14/2015
Wednesday Negative Staining
Lab #1
Smear Preparatation/Simple Stain
1/12/2015

1/19/2015

Monday

Monday

Read Intro and Ex. 1-3

Ex. 3-1
Ex. 3-10
Ex. 3-5
Ex. 3-4

MLK Holiday

Staining Methods
1/21/2015
Wednesday Smear Preparation
Lab #2
- Gram Stain
Spore Stain: Schaeffer-Fulton
Acid Fast Stain: Ziehl-Nielsen
1/26/2015
Monday
- Manual error: steam for 10 minutes
Lab #3
Gram Stain Practice
Culture Media Prep: read
Pure Culture Technique:
1/28/2015
Wednesday - Streak Plate Method
Lab #4
- Pour Plate Method
2/2/2015
Lab #5

Lab Exercises

Selective Media: PEA, DES, EMB, BA

Monday

Check streak & Pour plates from previous lab

Motility
Aseptic Techniques: Pipetting
Bacterial Population Counts:
2/4/2015
Wednesday Viable Plate Count: (Standard Plate Count)
Lab #6
Direct Microscopic Count
Results of selective media
Cultural Characteristics of Bacteria
Physiological Characteristics of Bacteria:
Hydrolysis, Starch, Milk, Lipase, Urea, Litmus,
2/9/2015
Monday Gelatinase
Lab #7
Biooxidations: Sugar Fermentation, Oxidase, and
Catalase
Methods for ID of unknown spp.
2/11/2015 Wednesday Read Results from Monday
Physiological Characteristics of Bacteria:
a) Hydrolysis; Tryptophane
2/16/2015
Monday
b) Biooxidations: MRVP, & Nitrate
Lab #8
c) Misc.: IMViC, H2S, Phenylalanine
2/18/2015 Wednesday Use of Bergey's Manual (read Ex. 41)

Ex. 3-6
Ex. 3-9
Ex. 3-7

Ex. 1-2
Ex. 1-4
Course Notes
Course Notes and Ex. 4-2, 44, 5-20
Ex 5-22, pg. 331
Appendix C: pg. 515-518
Course notes and Ex. 6-1
Course Notes/Handout
Course Notes
Ex. 2-2, 2.3, 2-4
Ex. 5-11, 5-13, 5-16, 5-12, 514, and pg 346
Ex. 5-2, 5-5, 5-4

Course Notes and 5-17

Ex. 5-3, 5-6
Ex. 5-3, 5-7, 5-9, 5-18, 5-17

2/23/2015
Lab #9

Monday

Gram Positive Pyogenic Cocci: (Day 1)

- Staphylococcus and Streptococcus
Minimum Inhibitory Conc.

Gram Positive Pyogenic Cocci: (Day 2)

Mannitol Salt Agar Plates, Staph Media 110, and
2/25/2015
Wednesday Blood Agar
Lab #10
Read MIC results
Lab Skills: Gram Stain, Isolation, Transfer
LAB MIDTERM EXAM GIVEN DURING THE
LECTURE
2/27/2015
Friday
Midterm
Lab Packet pages 1-7, Phenol Alanine Deaminase &

Read Course Notes

Ex. 5-20
Handout

Read course notes

Ex. 4-1, 5-20
Handout

Litmus Milk (pg 8) & IMViC (pg 9) & Gelatinase

Gram Positive Pyogenic Cocci (Day 3)

Test for Nitrate Reduction; Catalase; DNAse;
Monday
Coagulase
Gram Negative Intestinal Pathogens
Read Results from Day 3 Gram + Cocci
Inoculate Suspected Salmonella sp into KIA, SIM,
3/4/2015
Wednesday
Urea and read results Friday
Lab #12
Mycology Inoculations
3/9-3/14
Spring Break
Control of Microorganisms
- Temperature: effects on growth
- pH and Microbial Growth
- Osmotic Pressure and aw
3/16/2015
Monday
Lab #13
- Ultraviolet Light
Thioglycollate
Read Mycology results
Discussion of Gram Negative Intest. Patho.
Results of Lab 13
Bacteriological Examination of Water
3/18/2015
Wednesday Multiple Tube Method (MPN)
Lab #14
Demonstration: Filter Membrane Method
Lab skills make-up
Confirmed Test Sanitary Analysis of Water
Evaluation of Antisepsis
Antimicrobial Sensitivity Testing: Kirby-Bauer
3/23/2015
Monday
Method
Lab #15
Unknowns: Streak Mixture for Isolation and Gram
Stain
Completed Test Sanitary Analysis of Water
Read KB and Antisepsis
3/25/2015
Wednesday Unknowns Continued: pick isolated colonies &
Lab #16
inoculate slants: restreak if not isolated
Lab Skills: Make-up
3/30/2015
Monday Unknowns Continued: Biochemical Tests
Lab #17
Unknowns Continued
4/1/2015
Wednesday
3/2/2015
Lab #11

Course Notes
Ex. 5-4, 5-6, 5-15, 5-21
Course Notes, Ex.4-4, 4-6
Course Notes
Ex. 5-12, 5-17, 5-18
Handout

Ex. 2-8
Ex. 2-9
Ex. 2-10
Ex. 2-11
Ex. 2-6
Handout

Course Notes and Ex. 7.6

Ex. 7.5
Course Notes and Ex. 7.6
Course Notes & Ex. 2-12
Ex. 7-2
Course Notes & Ex. 9-1,2,3
Course Notes and Ex. 7-6
Course Notes & Ex. 9-1,2,3
Course Notes & Ex. 9-1,2,3
Course Notes & Ex. 9-1,2,3

4/1/2015
Wednesday
Lab skills make-up
Lab #18
4/6/2015
Course Notes & Ex. 9-1,2,3
Monday Unknowns Continued
Lab #19
Lab
Skills:
Make-up
4/8/2015 Wednesday
4/13/2015
Monday UNKNOWNS DUE
4/15/2015 Wednesday LAB PRACTICAL DURING LAB SESSION
4/20/2015
Monday Clean out the bins and drawers and last chance for skills make-ups