Code No: 34052 R05 Set No.

2
II B.Tech II Semester Supplimentary Examinations,February 2010
INSTRUMENTAL METHODS OF ANALYSIS
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. Define the term phase contrast. How is this principle exploited in the Phase
contrast microscope? [16]

2. (a) Write with suitable examples the shielding effects involved in NMR spec-
troscopy.
12
(b) Why C,16 O,18 O,32 S do not show NMR spectra? [8 × 2 = 16]

3. Compare & contrast the principles, applications of preparative & analytical

centrifugation technique. [16]

4. (a) Narrate the principle involved in ESR.

(b) Explain briefly the ESR instrument with a neat labelled diagram. [8 × 2 = 16]

5. (a) What is the process of excitation in emission spectroscopy?

(b) Discuss various excitation sources used in emission spectroscopy. [8 × 2 = 16]

6. (a) Give the basic principle involved in analytical centrifuge?

(b) What are the important applications of this technique in biochemistry?[8×2=16]

7. (a) An unknown organic compound gave molecular ion peak at m/e = 136. Two
possible structures corresponding to m/e =136 are C10 H16 (I) & C10 H2 N (II).
Which of the structures can be eliminated on the basis of nitrogen rule?
(b) Discuss some of the important features of the mass spectra of amines.
[8 × 2 = 16]

8. How would you test to determine water quantitatively in each of the following
circumstances?

(a) Water vapour in tanks of compresed H2 or O2 .

(b) Water dissolved in pure chloroform or ether.
(c) Water content of the atmosphere in a sealed space vehicle.
(d) Water collected in the bottom of a large gasoline storage tank.

Outline a procedure where possible. [4 × 4 = 16]

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Code No: 34052 R05 Set No. 4
II B.Tech II Semester Supplimentary Examinations,February 2010
INSTRUMENTAL METHODS OF ANALYSIS
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. (a) Explain:
i. Rayleigh scattering
ii. Raman scattering.
(b) Give the basic design of a spectrophotometer. [8 × 2 = 16]

2. (a) Why electron spin resonance is also called para magnetic resonance?
(b) Graphite can also be studied with ESR spectra. Why? [8 × 2 = 16]

3. Write an account on various types of centrifuges at laboratory levels? [16]

4. Discuss the principle & important applications of atomic absorption spectroscopy?

[16]

5. (a) Draw a neat labeled diagram of phase contrast microscope.

(b) Describe the function of every part involved in it. [8 × 2 = 16]
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6. (a) What is the difference between proton NMR & C NMR?
13
(b) Why C NMR spectra are difficult to record? [8 × 2 = 16]

7. (a) Discuss in brief the basic principles of mass spectrometry

(b) In the mass spectrum of ethanol, some of the prominent peaks appear at m/e
46,45,31,29,17&15. What are the most probable species responsible for these
peaks. [8 × 2 = 16]

8. Estimate the absolute deviation & the coefficient of variation for the results of the
following calculations. Round each result so that it contains only significant digits.
The number in parentheses are absolute standard deviations.

(a) Y = 19.97 (+- 0.04)+0.0030 (+- 0.0001)+ 1.29 (+-0.08) = 21.263

Y =143(+−6)−64(+−3)
(b) 1249(+−1)+77(+−8)
= 5.9578 × 10−2
(c) Y =67.1 (+/- 0.3) × 1.03 (+/- 0.02 ) ×10−17 = 6.9113 ×10−16 [16]

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Code No: 34052 R05 Set No. 1
II B.Tech II Semester Supplimentary Examinations,February 2010
INSTRUMENTAL METHODS OF ANALYSIS
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. Write a brief account on :

(a) Rate zonal centrifugation

(b) Isopycnic
(c) Centrifugal elutriation. [5+5+6]

2. (a) What is the difference between fluorescence & phosphorescence?

(b) How does an increase in quantum yield affect the intensity of fluorescence?
[8 × 2 = 16]

3. What is ESR spectrum. How it is used in the determination of reaction rates &
mechanisms. [16]

4. Write a brief note on the following:

(a) negative staining

(b) shadowing
(c) freeze- etching. [5+5+6]

5. Write an essay on uv-visible spectrophotometric methods employed in analytical

methods. [16]

6. (a) Define the term sensitivity & detection limits of instruments

(b) Write an account on detection & identification limits for analytical methods.
[16]

7. (a) Calculate the NMR frequency of 19F ina magnetic field of 2.3487τ . Given
gn = 5.255&βn = 5.05 × 10−27 JT −1 .
(b) Describe NMR spectrum of ethanol & explain the position of each band.
[8 × 2 = 16]

8. Write notes on:

(a) bainbridge mass spectrograph.

(b) dempster’s mass spectrometer. [8 × 2 = 16]

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Code No: 34052 R05 Set No. 3
II B.Tech II Semester Supplimentary Examinations,February 2010
INSTRUMENTAL METHODS OF ANALYSIS
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. (a) Discuss the basic principle of infra red spectroscopy?

(b) Briefly describe the scanning of infrared spectrum of an organic compound.
[8 × 2 = 16]

2. Differentiate between the following terms:

(a) Band spectra & Line spectra.

(b) Fluorescence & Phosphoroscence
(c) Molar absorptivity & Absorptivity
(d) Stokes shift & Bathochromic shift. [4 × 4 = 16]

3. (a) Define the term centrifugation.What is differential centrifugation?

(b) Explain the technique with a suitable example. [8 × 2 = 16]

4. (a) What do you mean by saturation

(b) What are the conditions on which saturation depends? [8 × 2 = 16]

5. (a) How does the scanning electron microscope operate?

(b) In what way does it function differ from that of TEM?. [8 × 2 = 16]

6. (a) Give the principle involved in NMR spectroscopy.

(b) Write a brief note on multidimensional NMR? [8 × 2 = 16]

7. Explain the difference between :

(a) Accuracy & Precision

(b) Mean & Median
(c) Variance & Standard deviation
(d) Constant & Proportional error. [4 × 4 = 16]

8. (a) Why the technique of atomic absorption is only limited to metals?

(b) What factors are responsible for the precision of atomic absorption
measurements? [8 × 2 = 16]

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