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Microbiological, physico-chemical
and sensorial quality of small-scale
produced stirred yoghurt on the
market in Kampala city, Uganda
Quality of
yoghurt in
Uganda
409
1. Introduction
In Uganda today, commercial processing of most food products is no longer a preserve
of large and reputable companies. Many small-scale processors have now joined the
food industry, a trend that has partly been driven by the need for individuals to create
their own jobs in order to earn a living or to supplement their meagre incomes. With
a modest capital, one quickly establishes a small-scale business, usually within their
homes. A number of yoghurt brands on the market in Kampala city and other parts of
the country have actually been born this way. Since such businesses often operate
without quality management systems in place, the quality of their products may be
questionable.
The authors appreciate the technical assistance rendered by the technicians in the Departments
of Food Science & Technology and Animal Science, Faculty of Agriculture, Makerere University,
Kampala.
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Table I.
Microbial counts of
different yoghurt brands
in Kampala
Yoghurt brand
Lactobacili
Coliforms
FD Control
OF
KA
GD
WM
9.67 0.04
7.12 0.54
8.85 0.21
9.25 0.41
8.97 0.19
9.71 0.08
6.80 0.11
6.65 0.07
6.29 0.60
6.62 0.12
ND
3.29 0.16
1.35 0.06
3.44 1.51
3.15 0.62
ND
4.16 1.33
3.11 0.13
3.50 0.13
3.38 0.16
Notes: Values are mean standard deviation of four independent determinations; ND not
detected
their presence in the final product is a result of insufficient heat processing or post heat
treatment contamination.
3.2 Physico-chemical analysis
The physico-chemical properties of the different yoghurt brands are shown in Table II.
Significant differences (p < 0.05) were observed for most of the parameters with the
exception of protein, ash and titratable acidity.
3.2.1 Fat. The fat content ranged from undetectable limits (brand OF) to 3.31 per cent
(Table II). Other authors have reported values ranging between 2.17 and 4.51 (Younus
et al., 2002; Haj et al., 2007). WM had significantly higher contents of fat compared to the
rest of the brands. Brands KA (1.45 1.62) and GD (1.13 1.3) had high standard
deviations, possibly indicating wide variations between batches. The variation in fat
could be attributed to variations in the raw milk, treatments to reduce fat or practices
such as adulteration of milk by adding water. Basing on fat content, yoghurts can be
classified into whole milk yoghurt (min. 3 per cent fat), fat reduced (0.5-3 per cent fat)
or fat free (<0.5 per cent fat) (COMESA, 2009). Staff (1998) also reports the fat content
based categorisation of yoghurt in various countries. Whereas the control, FD, is
marketed as reduced fat yoghurt and labelled as such, there was no such labelling on the
packages of the small-scale produced brands.
3.2.2 Protein. The protein content did not differ significantly and ranged from 2.32
to 2.99 per cent (Table II). Values in the range of 2.66 to 4.16 have been reported (Haj
et al., 2007; Alakali et al., 2008; Khan et al., 2008). The Codex standard for milk and
fermented milks specifies a minimum of 2.7 per cent protein (Codex, 2003). According
to Kroger (1975), protein contents below 3.4 per cent may be associated with syneresis,
thus necessitating fortification or the use of stabilisers.
3.2.3 Ash. The ash content of the small-scale produced yoghurts, which ranged from
0.55 to 0.65 per cent, was not significantly different from the control (Table II). These
values are close to those reported by other authors (Haj et al., 2007; Mehmood et al., 2008).
3.2.4 Carbohydrate. The carbohydrate content (Table II) of the small-scale produced
yoghurt brands was not significantly different from the control. Values ranged from
10.62 per cent (WM) to 14.57 per cent (GD). These values were close to those reported
by Haj et al. (2007) but higher than those given by other studies (Khan et al., 2008;
Quality of
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Property
FD (Control)
OF
Yoghurt brands
KA
GD
WM
Fat (%)
Protein (%)
Ash (%)
Carbohydrate (%)
Total solids (%)
SNF (%)
TSS ( Brix)
pH
Viscosity (%)
Syneresis (%)
0.90 0.34a
2.99 0.54a
0.77 0.01a
12.38 0.42ab
17.28 0.17a
16.38 0.51a
13.65 0.19a
3.93 0.02a
64.6 1.41a
29.47 0.56a
ND
2.50 0.17a
0.62 0.01a
10.92 0.84b
13.92 0.46d
13.92 0.46b
9.6 0.23d
4.35 0.04c
57.00 0.85ac
35.28 1.97ac
1.45 1.62a
2.34 0.08a
0.65 0.00a
11.38 1.60ab
15.48 0.41c
14.03 1.35b
12.15 0.10c
3.96 0.04a
53.85 3.61bc
41.30 9.23c
1.13 1.3a
2.32 0.64a
0.62 0.02a
14.57 0.55a
18.57 0.10b
17.45 1.27a
14.3 0.35b
4.16 0.03b
47.25 2.47b
53.63 0.38b
3.31 0.33b
2.38 0.17a
0.55 0.05a
10.62 0.01b
17.00 0.29a
13.69 0.62b
13.2 0.16a
4.66 0.01d
58.50 1.27ac
52.60 0.36b
Table II.
Notes: Values are mean standard deviation of four independent determinations; values in a row
with similar superscript letters are not significantly different (p > 0.05); ND not detected
Physiochemical
properties of different
yoghurt brands
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Mehmood et al., 2008). Since the carbohydrate content in this study was obtained by
subtraction (Carbohydrate TSS (Protein Fat Ash)), it would be misleading
to refer to it as the lactose content as other authors have done previously (Haj et al.,
2007; Khan et al., 2008). This is because the difference could also account for the added
stabiliser and any other adjuncts that processors may add to increase the total solids.
3.2.5 Total solids. The total solids content ranged between 13.92 and 18.57 per cent
(Table II). WM was comparable to the control while brands OF and KA had
significantly lower values. These values are in agreement with those reported by other
studies (Haj et al., 2007; Alakali et al., 2008; Khan et al., 2008). Besides the concentration
and fortification of the yoghurt base, the type and amount of stabiliser used may also
influence the total solids content (Alakali et al., 2008; Mehmood et al., 2008).
3.2.6 Solids-non-fat. The solids-non-fat (SNF) content ranged between 13.69 and
17.45 per cent (Table II). With the exception of GD, the SNF values of the other brands
were significantly lower than the control. All these values are, however, comparable
with those reported by other authors (Younus et al., 2002; Haj et al., 2007; Alakali et al.,
2008; Khan et al., 2008) and above the proposed minimum of 8.2 per cent for East
and Southern Africa (COMESA, 2009). Values between 12 and 15 per cent may be
necessary to achieve desirable texture (Staff, 1998). Variation in SNF is influenced by
both the fat content and the total solids (Kroger, 1975).
3.2.7 Total soluble solids. Total soluble solids (TSS) ranged from 9.6 to 14.3 per cent.
There were significant differences between all the samples with the exception of FD and
WM. The levels of TSS could be used to compare the sweetness of different yoghurt
brands.
3.2.8 pH. The pH values were in the range 3.93 to 4.66 (Table II). Although there
were significant differences in pH values, all brands, with the exception of WM, had
their pH values below 4.5. Other authors have presented similar findings (Younus et al.,
2002; Haj et al., 2007; Mehmood et al., 2008). According to Kroger (1975) an optimum
final pH of 4.1 to 4.4 is desirable. This not only facilitates flavour production by the
Lactobacilli and development of a good coagulum (Kroger, 1975) but also prevents the
growth of spoilage and pathogenic microbes (Downes and Ito, 2001).
3.2.9 Titratable acidity. The values of titratable acidity did not differ significantly
and ranged from 0.31 to 0.37 per cent. Values close to these have been reported for rice
and soy yoghurts (Haj et al., 2007; Jimoh and Kolapo, 2007). These values are, however,
lower than those obtained by other authors (Younus et al., 2002; Haj et al., 2007;
Mehmood et al., 2008). A minimum acidity of 0.6 per cent is recommended (Codex, 2003)
since the formation of a coagulum is reported to start at this level of acidity (Kroger,
1975). A lower acidity results in less milk protein coagulation and thus lower viscosity.
The level of acidity should also be based on market research as consumers may prefer
different levels of acidity (Kroger, 1975; Tamime and Robinson, 1985).
3.2.10 Viscosity. The viscosity of the yoghurt brands ranged from 47.25 to 64.6 per
cent (Table II). The viscosity of brands OF and W was similar to the control while those
of KA and GD was significantly lower. These values are close to those observed by Haj
et al. (2007) for stirred yoghurts in Sudan but lower than those reported by Younus et al.
(2002) for yoghurts from Pakistan. Viscosity, which is a very important quality
parameter of yoghurt, is influenced by total solids, fat content, processing and addition
of stabilisers (Najgebauer-Lejko et al., 2007). Viscosity can be improved by increasing
milk solids through heating or fortification and the use of stabilisers (Kroger, 1975).
Much as the increase in solids is vital, it can be noted that yoghurt brand GD, which
had the highest total solids content, had the least viscosity while brand OF, which
had the lowest total solids, had the higher viscosity value. Therefore in selecting
fortificants, consideration should be given to their effects on viscosity.
3.2.11 Syneresis. Average syneresis ranged from 29.47 in the control to 53.63 per cent
in brand GD. Syneresis in brand OF was not significantly different from the control.
Brands GD and WM had the highest levels of syneresis. Values of 22.8 to 29.64 per cent
have been reported for commercial yoghurt in Pakistan (Younus et al., 2002). Syneresis is
an undesirable phenomenon in yoghurt as it can result in the leakage of water from the
product (Kroger, 1975). It may result from insufficient heat treatment of the milk (Ranken
and Kill, 1993; Staff, 1998). Heat treatment promotes protein denaturation, which
increases water binding and viscosity. Other causes of syneresis include rough handling
of the coagulum, low protein, low fat and low mineral content, and low acid formation.
On top of addressing these causes, syneresis can also be reduced by the use of stabilisers
(Kroger, 1975; Younus et al., 2002). The extent of syneresis is also influenced by the type
of fortificant used (Athar et al., 2000).
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Figure 1.
Scores for consumer
acceptability and
willingness to buy for
different yoghurt brands
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appearance of all the small-scale produced brands was liked as much as the control
with the exception of WM, which was neither liked nor disliked.
In case of aroma acceptability, brands KA and OF received similar but significantly
higher scores than GD which was disliked. One of the panellist noted that GD had a
burnt milk odour. This could have contributed to its low scores for aroma acceptability.
Aroma (19/23) was ranked as the most important quality attribute of yoghurt followed
by taste (13), viscosity/mouth feel (11), colour (9), consistency (7) and appearance (4).
These observations are in line with earlier reports (Kroger, 1975; Khan et al., 2008;
Mehmood et al., 2008).
Brand OF received significantly higher scores for taste than KA, WM and GD.
The taste of all these brands was acceptable. The taste of GD was, however, disliked.
Panellists noted that the small-scale brands were generally too sweet. Brand GD
actually had the highest content of total soluble solids (Table II). Panellists also noted
that GD had an unpleasant, pungent and sour taste while WM, which neither liked
nor disliked, had a spoilt-milk taste. Brands KA and OF received significantly higher
acceptability scores for mouth feel compared to WM and GD, which were disliked.
Surprisingly, GD had the highest SNF content while that of WM was equivalent to both
OF and KA. Although the addition of stabilisers contributes to the SNF content, thus
affecting mouth feel, the type of stabiliser used also matters (Alakali et al., 2008). Some
panellists observed that WM had solid particles while both WM and GD were quite
thin. Brands GD and WM probably contained stabilisers with a low water holding
capacity or suffered mechanical damage as can be seen from their relatively high level
of syneresis (Table II). The panellists were more willing to buy brands OF and KA
and quite unwilling to purchase brands WM and GD. This can be attributed to the
consumer perception of the aroma, taste and mouth feel of these brands (Figure 1).
Most of the respondents (15/23) expressed disatisfaction with the quality of smallscaled yoghurts citing problems such as poor packaging (8), poor flavour (5) and
early spoilage (3). Other deficiencies noted include inconsistencies in taste, lack of
traceability, absence of expiry dates on the packages, excessive sweetness and often too
much use of flavourants. It was also observed that most yoghurt manufacturers do not
adhere to the general guidelines for labelling (Codex, 1985) especially in regards to
declaration of fat content, date marking and lot identification. The absence of lot
markings renders traceability impossible. Although yoghurt may be bought off the
shelf long before its expiry date, some processors may deliberately avoid date marking
as a safeguard against product rejection on the shelf. This is possible when consumers
realise that the product is close to or past its expiry date.
4. Conclusions
This study has shown that there are variations in the quality of small-scale produced
yoghurts on the market in Kampala with some of the brands being quite inferior. Some
of the quality problems include excessive sweetness and sourness, off odours, thin
consistency, syneresis, short shelf life and microbial contamination. Fungi and coliform
contamination in these yoghurts indicates insufficient process sanitation and also
raises concerns of consumer safety especially with regards to pathogens and
mycotoxins, respectively. The high fungi counts could be responsible for the problem
of short shelf life of these products. Because of improper labelling, the consumer is
not well informed. It also renders it impossible to execute product recall in cases of
defective products. It is consequently recommended that stakeholders should
strengthen surveillance and plan trainings for small-scale processors to ensure that
their products meet both quality and safety requirements.
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About the authors
I.M. Mukisa graduated with a BSc Food Science and Technology degree from Makerere
University, Kampala, Uganda in 2003. He worked as Food Quality Personnel in the fish industry
in Uganda from 2003 to 2005 before joining the Department of Food Science & Technology as a
Teaching Assistant. He completed a Master of Science degree in Food Technology from the
Katholieke Universiteit Leuven and Universiteit Gent (Belgium) in 2007. He subsequently
embarked on a PhD at the Norwegian University of Life Science in Norway. His PhD research
involves the development of starter cultures for Obushera, a traditional fermented beverage from
Uganda. He is currently an Assistant Lecturer in the Department of Food Science and
Technology Makerere University. His work involves lecturing, conducting and supervising
research. His areas of interest includes food microbiology, sensory evaluation and improvement
of local products. I.M. Mukisa is the corresponding author and can be contacted at:
ivanmukisa@agric.mak.ac.ug
R. Kyoshabire has just completed her four-year undergraduate course in Food Science and
Technology at Makerere University. This study was done as a fulfilment of part of the
requirements for the award of the BSc Food Science and Technology degree from Makerere
University.