Selectra I I

ACID PHOSPHATASE (ACP)
Continuous-spectrophotometric
SFBC
Instrument:
Principle of the method
SELECTRA-2
Acid phosphatase (ACP) catalyzes in acid medium the hydrolysis of the

phosphate group from -naphtyl phosphate. The -naphtol formed reacts with
a diazonium salt (Fast Red TR) originating a chromogen. The catalytic
concentration is determined from the rate of chromogen formation, measured
at 405 nm. Tartrate is used as a specific inhibitor of the prostatic fraction.
Samples
Reagent preparation
Working Reagent: Stopper the vial with the cap containing -naphtyl
phosphate and press the red button until the solute falls into the vial. Add 10
mL of Reagent A1 (Total ACP) or 10 mL of Reagent A2 (Non Prostatic ACP).
Cap and shake until dissolved. Stable for 10 days at 2-8C.
Performance characteristics
Serum.
Acid phosphatase is unstable in serum. In acidfied serum is stable for 6 days at 2-8C.
Linearity: up to 150 U/L.

Interferences: Hemolysis and bilirubin interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
ACP
Kinetic
405 nm
U/L
0
0 U/L
150 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 U/L
10 U/L
0 U/L
10 U/L
Correlation Factor
Correlation Offset
1.000
0.000
ACP
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
-0.050
3.000
-0.050
0.500
0.150
Yes
(...)
(...)
If the test is run without calibration enter

... Data entered by the operator
* assigned value of standard
FACTOR: 1480
20 L
10 L
289, 175 sec.
Version 0206
ALBUMIN
Spectrophotometric
BROMOCRESOL GREEN
Instrument:
SELECTRA-2
Reagent preparation
Samples
Albumin in the sample reacts with bromocresol green in acid medium forming a Reagent is ready to use.
coloured complex that can be measured by spectrophotometry.
Serum, plasma.
Stable for 6 days at 2-8C.
Linearity: up to 70 g/L.
Interferences: Hemoglobin (1 g/L) and bilirubin (25 mg/dL) interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Albumin
Endpoint
620 nm
g/L
1
0 g/L
70 g/L
(...)
3
1
*
(...)
No
Albumin
No
25 mL
270 L
250 L
4 L
3 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
35 g/L
50 g/L
35 g/L
50 g/L
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.180
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0207
ALKALINE PHOSPHATASE (ALP)
DEA BUFFER
Instrument:
SELECTRA 2
Reagent preparation
Samples
Alkaline phosphatase (ALP) catalyzes in alkaline medium the transfer of the

phosphate group from 4-nitrophenylphosphate to diethanolamine (DEA),
liberating 4-nitrophenol. The catalytic concentration is determined from the rate
of 4-nitrophenol formation, measured at 405 nm.
Serum, plasma.
Alkaline phosphatase in serum or plasma is stable for 7 days at 2-8C.
Heparin may be used as anticoagulant
Working Reagent: Dissolve the powder of a Reagent B vial with 20 mL of the

Reagent A bottle (if 10x20 mL size) or dissolve the contents of a Reagent B
vial with the entire volume of a Reagent A bottle (if 5x100 mL size).
Stable for 2 months at 2-8C.
-

Interferences: Fluoride, oxalate, citrate and EDTA as anticoagulants
interfere. Hemolysis interferes due to the alkaline phosphatase content in
red cells.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
ALP DEA
Kinetic
405 nm
U/L
0
0 U/L
690 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
90 U/L
280 U/L
90 U/L
280 U/L
Correlation Factor
Correlation Offset
1.000
0.000
ALP DEA
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
-0.050
3.000
-0.050
0.950
0.150
Yes
(...)
(...)

FACTOR: 2764
5 L
3 L
32, 175 sec.
Version 0012
ALKALINE PHOSPHATASE (ALP)
AMP BUFFER (IFCC)
Instrument:
SELECTRA 2
Reagent preparation
Samples
Alkaline phosphatase (ALP) catalyzes in alkaline medium the transfer of the

phosphate group from 4-nitrophenylphosphate to 2-amino-2-methyl-1-propanol
(AMP), liberating 4-nitrophenol. The catalytic concentration is determined from
the rate of 4-nitrophenol formation, measured at 405 nm.
Serum, plasma.
Alkaline phosphatase in serum or plasma is stable for 7 days at 2-8C.
Working Reagent: Dissolve the powder of a Reagent B vial with 20 mL of the

Reagent A bottle (if 10x20 mL size) or dissolve the contents of a Reagent B
vial with the entire volume of a Reagent A bottle (if 5x100 mL size).
-

interfere. Hemolysis interferes due to the alkaline phosphatase content in
red cells.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
ALP IFCC
Kinetic
405 nm
U/L
0
0 U/L
1200 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
26 U/L
117 U/L
26 U/L
117 U/L
Correlation Factor
Correlation Offset
1.000
0.000
ALP IFCC
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
-0.050
3.000
-0.050
0.950
0.150
Yes
(...)
(...)

FACTOR: 2764
5 L
3 L
32, 175 sec.
Version 0012
ALANINE AMINOTRANSFERASE (ALT)
IFCC
Instrument:
SELECTRA 2
Reagent preparation
Samples
Alanine aminotransferase (ALT or GPT) catalyzes the transfer of the amino

group from alanine to 2-oxoglutarate, forming pyruvate and glutamate. The
catalytic concentration is determined from the rate of decrease of NADH,
measured at 340 nm, by means of the lactate dehydrogenase (LDH) coupled
reaction.
Serum.
Alanine aminotransferase in serum is stable for 7 days at 2-8C.
MONO MODE.- Working Reagent: Pour the contents of the Reagent B into the
Reagent A bottle. Mix gently.
DUAL MODE.- Reagent 1: Use the Reagent A.
Reagent 2: Use the Reagent B.
-
Interferences: High pyruvate in the sample will consume NADH during the
delay time before measurements, reducing the linearity of the method.
Linearity: Up to 500 U/L.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Delay, min. Time
ALT
Kinetic
340 nm
U/L
0
0 U/L
500 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 U/L
41 U/L
0 U/L
41 U/L
Correlation Factor
Correlation Offset
1.000
0.000
ALT
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
0.800
2.500
0.800
2.200
0.150
Yes
(...)
(...)
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
0.800
2.500
0.800
2.200
0.100
Yes
(...)
(...)

FACTOR: -3466
12 L
6 L
70, 176 sec.
ALT
No
25 mL
225 L
225 L
12 L
6 L
25 mL
25 L
25 L
No
No
77, 132 sec.
Version 0012
-AMYLASE
DIRECT SUBSTRATE
Instrument:
SELECTRA 2
Reagent preparation
Samples
-Amylase catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-maltotrioside Reagent is ready to be used.

(CNP-G3) to 2-chloro-4-nitrophenol (CNP). The catalytic concentration is
determined from the rate of 2-chloro-4-nitrophenol formation, measured at 405
nm.
Serum, plasma, urine.
-Amylase in serum, plasma or urine is stable for 5 days at 2-8C.
Linearity: up to 1300 U/L (serum) or 2600 U/L (urine).

interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
AMYL DIRECT
Kinetic
405 nm
U/L
0
0 U/L
1300 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 U/L
60 U/L
0 U/L
60 U/L
Correlation Factor
Correlation Offset
1.000
0.000
AMYLASE DIR.
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
-0.050
3.000
-0.050
0.250
0.150
Yes
(...)
(...)

FACTOR: 3292
5 L
3 L
32, 175 sec.
Version 0012
-AMYLASE
IFCC
Instrument:
SELECTRA-2
Reagent preparation
-Amylase catalyzes the hydrolysis of 4-nitrophenyl-maltoheptaosideethylidene to smaller oligosacharides which are hydrolyzed by -glucosidase
liberating 4-nitrophenol. The catalytic concentration is determined from the rate
of 4-nitrophenol formation, measured at 405 nm.
Samples
Working Reagent: Pour the contents of the Reagent B into the Reagent A
bottle. Mix gently. Other volumes can be prepared in the proportion: 4 mL
Reagent A + 1 mL Reagent B.

-Amylase in serum or plasma is stable for 1 month at 2-8C. Use heparin or EDTA as anticoagulant.
-Amylase in urine is stable for 1 month at 2-8C if pH is adjusted to
approximately 7 before storage.
Linearity: up to 1300 U/L (serum) or 2600 U/L (urine).

Interferences: Lipemia (triglycerides 10 g/L) and blirubin (20 mg/dL) do
not interfere. Hemoglobin (10 g/L) interfere. Other drugs and substances
may interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
AMYL
Kinetic
405 nm
U/L
0
0 U/L
1300 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
28 U/L
100 U/L
28 U/L
100 U/L
Correlation Factor
Correlation Offset
1.000
0.000
AMYLASE
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
-0.100
3.000
-0.100
3.000
0.300
Yes
(...)
(...)

FACTOR: 3042
8 L
6 L
129, 195 sec.
Version 0204
ASPARTATE AMINOTRANSFERASE (AST)
IFCC
Instrument:
SELECTRA 2
Reagent preparation
Samples
Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino

group from aspartate to 2-oxoglutarate, forming oxalacetate and glutamate.
The catalytic concentration is determined from the rate of decrease of NADH,
measured at 340 nm, by means of the malate dehydrogenase (MDH) coupled
reaction.
Serum.
Aspartate aminotransferase in serum is stable for 7 days at 2-8C.
MONO MODE.- Working Reagent: Pour the contents of the Reagent B into the
Reagent A bottle. Mix gently.
DUAL MODE.- Reagent 1: Use the Reagent A.
-
Interferences: High pyruvate in the sample will consume NADH during the
delay time before measurements, reducing the linearity of the method.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Delay, min. Time
AST
Kinetic
340 nm
U/L
0
0 U/L
500 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 U/L
42 U/L
0 U/L
42 U/L
Correlation Factor
Correlation Offset
1.000
0.000
AST
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
0.800
2.500
0.800
2.200
0.150
Yes
(...)
(...)
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
0.800
2.500
0.800
2.200
0.100
Yes
(...)
(...)

FACTOR: -3465
12 L
6 L
70, 176 sec.
AST
No
25 mL
225 L
225 L
12 L
6 L
25 mL
25 L
25 L
No
No
77, 132 sec.
Version 0012
TOTAL BILIRUBIN
Spectrophotometric
DIAZOTIZED SULFANILIC
Instrument:
SELECTRA 2
Reagent preparation
Samples
Total bilirubin in the sample reacts with diazotized sulfanilic in acid medium Working Reagent: Transfer the contents of one Reagent B vial into a Reagent
forming a coloured complex that can be measured by spectrophotometry. Both A-T bottle. Mix thoroughly.
direct (conjugated with glucuronate) and indirect (unconjugated) bilirubin Stable for 20 days at 2-8C.
couple with diazo in the presence of cetrimide. The terms direct and total
refer to the reaction characteristics of serum bilirubin in the absence or
presence of solubilizing (accelerating) reagents, and are only approximately
equivalent to the conjugated and unconjugated fractions.
Serum.
Stable for 20 days at 2-8 C and protected from light.
Linearity: Up to 15 mg/dL.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Bilirubin
Endpoint
546 nm
mg/dL
2
0 mg/dL
15 mg/dL
(...)
3
1
*
(...)
No
T-BILIRUBIN
No
25 mL
250 L
250 L
25 L
15 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0.00 mg/dL
1.10 mg/dL
0.00 mg/dL
1.10 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.150
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
CALCIUM
Spectrophotometric
ARSENAZO III
Instrument:
SELECTRA 2
Reagent preparation
Samples
Calcium in the sample reacts with arsenazo III forming a coloured complex that Reagent is ready to be used.
can be measured by spectrophotometry.
Serum, heparinized plasma, urine.
Calcium in serum or plasma is stable for 10 days at 2-8 C.
Anticoagulants other than heparin should not be used.
Interferences: Hemoglobin (1.5 g/L), bilirubin (20 mg/dL), magnesium (10
mg/dL) and phosphate (20 mg/dL) do not interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Calcium
Endpoint
620 nm
mg/dL
2
0 mg/dL
18 mg/dL
(...)
3
1
*
(...)
No
CALCIUM
No
25 mL
300 L
300 L
4 L
2 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
9.0 mg/dL
10.7 mg/dL
9.0 mg/dL
10.7 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
2.000
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
CALCIUM
Spectrophotometric
METHYLTHYMOL BLUE
Instrument:
SELECTRA 2
Reagent preparation
Samples
Calcium in the sample reacts with methylthymol blue in alkaline medium Working Reagent: Mix equal volumes of Reagent A and Reagent B. Mix
forming a coloured complex that can be measured by spectrophotometry. thoroughly.
Hydroxyquinoline is included in the reagent to remove magnesium interference. Stable for 2 days at 2-8C.
Serum, heparinized plasma, urine.
Calcium in serum or plasma is stable for 10 days at 2-8 C.
Interferences: Hemoglobin (1.5 g/L), bilirubin (20 mg/dL), magnesium (10
mg/dL) and phosphate (20 mg/dL) do not interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Calcium
Endpoint
620 nm
mg/dL
2
0 mg/dL
15 mg/dL
(...)
3
1
*
(...)
No
CALCIUM
No
25 mL
300 L
300 L
3 L
2 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
9.0 mg/dL
10.7 mg/dL
9.0 mg/dL
10.7 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.700
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
CHOLINESTERASE
BENZOYLCHOLINE
Instrument:
SELECTRA 2
Reagent preparation
Samples
Cholinesterase (CHE) catalyzes the hydrolysis of benzoylcholine to choline and

benzoic acid. The catalytic concentration is determined from the rate of
quinoneimine formation, measured at 500 nm, by means of the choline and
peroxidase coupled reactions.
Serum, heparinized plasma or EDTA plasma.
Cholinesterase in serum or plasma is stable for 7 days at 2-8C.
Working Reagent: Reconstitute the contents of a Reagent B vial with 3 mL (if

20x3 mL size), 15 mL (if 10x15 mL size) or 50 mL (if 4x50 mL size) of Reagent
A. Swirl gently.
-
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
Cholinesterase
Kinetic
505 nm
U/L
0
0 U/L
6500 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
2150 U/L
4950 U/L
Correlation Factor
Correlation Offset
1.000
0.000
Cholinesterase
No
25 mL
300 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
-0.050
3.000
-0.050
0.950
0.150
Yes
(...)
(...)

FACTOR: 28000
2 L
2 L
188, 175 sec.
Version 0203
CHOLESTEROL
Enzymatic-spectrophotometric
CHOLESTEROL OXIDASE/PEROXIDASE
Instrument:
SELECTRA 2
Reagent preparation
Samples
Free and esterified cholesterol in the sample originates, by means of some Reagent is ready to be used
coupled reactions, a coloured complex that can be measured by
spectrophotometry.
Serum or plasma.
Heparin, EDTA, oxalate and fluoride may be used as anticoagulants.
Interferences: Hemoglobin (3 g/L), ascorbic acid (0.3 mmol/L) and

bilirubin (0.25 mmol/L) interfere. Lipemia does not affect results.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Cholesterol
Endpoint
505 nm
mg/dL
0
0 mg/dL
1000 mg/dL
(...)
3
1
*
(...)
No
Cholesterol
No
25 mL
300 L
300 L
3 L
2 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
123 mg/dL
270 mg/dL
123 mg/dL
243 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.150
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
CREATINE KINASE (CK)
IFCC
Instrument:
SELECTRA 2
Reagent preparation
Samples
Creatine kinase (CK) catalyzes the phosphorylation of ADP, in the presence of Working Reagent: Empty the contents of a Reagent B bottle into a Reagent A
creatine phosphate, to form ATP and creatine. The catalytic concentration is bottle. Swirl gently.
determined from the rate of NADPH formation, measured at 340 nm, by means Stable for 15 days at 2-8C.
of the hexokinase (HK) and glucose-6-phosphate coupled reactions.
Serum.
Creatine kinase in serum is stable for 7 days at 2-8C.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
CK
Kinetic
340 nm
U/L
0
0 U/L
730 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
38 U/L
174 U/L
26 U/L
140 U/L
Correlation Factor
Correlation Offset
1.000
0.000
CK
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
-0.050
3.000
-0.050
0.500
0.150
Yes
(...)
(...)

FACTOR: 3465
12 L
6 L
188, 175 sec.
Version 0101
CREATININE
Kinetic-spectrophotometric
ALKALINE PICRATE
Instrument:
SELECTRA 2
Reagent preparation
Samples
Creatinine in the sample reacts with picrate in alkaline medium forming a Reagent 1: Use the Reagent A.
coloured complex. The complex formation rate is measured in a short period to Reagent 2: Use the Reagent B.
avoid interferences.
Creatinine in serum or plasma is stable for 24 hours at 2-8C.
Interferences: Hemoglobin (0.1 g/L), bilirubin (10 mg/dL), protein and

ketonic bodies do not interfere.
Linearity: Up to 20 mg/dL (serum or plasma).
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Point one, two
Creatinine
Twopoint
505 nm
mg/dL
1
0 mg/dL
20 mg/dL
(...)
3
1
*
(...)
No
Creatinine
No
25 mL
190 L
190 L
25 L
12 L
25 mL
65 L
65 L
No
No
24, 103 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0.6 mg/dL
1.1 mg/dL
0.5 mg/dL
0.9 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.100
3.000
-0.100
0.350
0.100
Yes
(...)
(...)

Version 0012
GAMMA-GLUTAMYLTRANSFERASE ( -GT)
IFCC
Instrument:
SELECTRA 2
Reagent preparation
Samples
Gamma-glutamyltansferase ( -GT) catalyzes the transfer of the -glutamyl Working Reagent: Pour the contents of the Reagent B into the Reagent A
group from -glutamyl-3-carboxy-4-nitroanilide to glycylglycine, liberating 3- bottle. Mix gently.
carboxy-4-nitroaniline. The catalytic concentration is determined from the rate Stable for 2 months at 2-8 C.
of 3-carboxy-4-nitroaniline formation.
Serum.
Gamma-glutamyltransferase in serum is stable for 5 days at 2-8 C.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
GGT
Kinetic
405 nm
U/L
0
0 U/L
300 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
15 U/L
86 U/L
10 U/L
40 U/L
Correlation Factor
Correlation Offset
1.000
0.000
GGT
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
-0.050
3.000
-0.050
0.900
0.150
Yes
(...)
(...)

FACTOR: 1111
25 L
12 L
32, 175 sec.
Version 0207
GLUCOSE
GLUCOSE OXIDASE/PEROXIDASE
Instrument:
SELECTRA 2
Reagent preparation
Samples
Glucose in the sample originates, by means of some coupled reactions, a Reagent is ready to be used
Serum or plasma.
Interferences: Hemoglobin (0.3 g/L), ascorbic acid (10 mg/dL) and

bilirubin (15 mg/dL) interfere. Moderate lipemia does not affect the
results.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Glucose
Endpoint
505 nm
mg/dL
0
0 mg/dL
500 mg/dL
(...)
3
1
*
(...)
No
Glucose
No
25 mL
300 L
300 L
3 L
2 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
76 mg/dL
110 mg/dL
76 mg/dL
110 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.150
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
HDL CHOLESTEROL
Precipitation/Enzymatic-spectrophotometric
PHOSPHOTUNGSTATE/Mg 2+-CHOLESTEROL OXIDASE/PEROXIDASE
Instrument:
SELECTRA-2
Reagent preparation
Samples
Very low density lipoproteins (VLDL) and low density lipoproteins (LDL) in the Reagent B is ready to be used.
sample precipitate with phosphotungstate and magnesium ions. The
supernatant contains high density lipoproteins (HDL). The HDL cholesterol is
then spectrophotometrically measured by means of some coupled reactions.
Serum or plasma. Stable for 7 days at 2-8C.
Precipitation Procedure:
1.
Pipette into labelled centrifuge tubes:
Sample
0.2 mL
Reagent A
0.5 mL
2.
Mix thoroughly and let stand for 10 minutes at room temperature.
3.
Centrifuge at a minimum of 4000 r.p.m. for 10 minutes.
4.
Carefully collect the supernatant.
Linearity: up to 200 mg/dL.

Interferences: Hemoglobin (1 g/L), bilirubin (10 mg/d/L) and acid ascorbic
(0.1 mmol/L) interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
HDL-C
Endpoint
505 nm
mg/dL
0
0 mg/dL
200 mg/dL
(...)
3
1
*
(...)
No
HDL-C
No
25 mL
260 L
260 L
13 L
2 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
30 mg/dL
60 mg/dL
40 mg/dL
70 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.200
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0206
IRON
Spectrophotometric
CHROMAZUROL B
Instrument:
SELECTRA 2
Reagent preparation
Samples
Ferric ions in the sample react with chromazurol B and Reagent is ready to be used
cetyltrimethylamoniumbromide (CTAB) forming a coloured complex that can be
measured by spectrophotometry.
Serum or heparinized plasma.
Interferences: Do not use hemolyzed sera.

Linearity: Up to 500 g/dL.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Iron
Endpoint
620 nm
g/dL
0
0 g/dL
500 g/dL
(...)
3
1
*
(...)
No
Iron
No
25 mL
250 L
250 L
12 L
12 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
70 g/dL
155 g/dL
55 g/dL
140 g/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.100
3.000
-0.100
3.000
0.100
No
(...)
(...)

Version 0012
IRON
Spectrophotometric
FERROZINE
Instrument:
SELECTRA 2
Reagent preparation
Samples
Transferrin-bound ferric ions in the sample are released by guanidinium and Working Reagent: Transfer the contents of one Reagent B vial into a Reagent
reduced to ferrous by means of hydroxylamine. Ferrous ions react with A bottle. Mix thoroughly.
ferrozine forming a coloured complex that can be measured by Stable for 6 months at 2-8C.
spectrophotometry.
Serum or heparinized plasma.

Linearity: Up to 1000 g/dL.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Iron Ferrozine
Endpoint
546 nm
g/dL
0
0 g/dL
1000 g/dL
(...)
3
1
*
(...)
No
Iron Ferrozine
Yes
25 mL
220 L
220 L
30 L
15 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
70 g/dL
155 g/dL
55 g/dL
140 g/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.150
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
LACTATE DEHYDROGENASE (LD/LDH)
PYRUVATE
Instrument:
SELECTRA 2
Reagent preparation
Samples
Lactate dehydrognase (LD or LDH) catalyzes the reduction of pyruvate by Working Reagent: Pour the contents of the Reagent B into the Reagent A
NADH, to form lactate and NAD+. The catalytic concentration is determined bottle. Mix gently.
from the rate of decrease of NADH, measured at 340 nm.
Serum or plasma.
Lactate dehydrogenase in serum or plasma is stable for 24 hours at 2-8C.
Heparin may be used as anticoagulant.
Interferences: Hemolysis interferes due

dehydrogenase concentration in red cells.
to
the
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
LDH
Kinetic
340 nm
U/L
0
0 U/L
1200 U/L
(...)
3
1
*
(...)
No
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
207 U/L
414 U/L
207 U/L
414 U/L
Correlation Factor
Correlation Offset
1.000
0.000
LDH
No
25 mL
250 L
250 L
Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
10%
-0.050
3.000
-0.050
2.000
0.150
Yes
(...)
(...)

FACTOR: -8095
5 L
3 L
32, 175 sec.
Version 0203
high
lactate
LDL CHOLESTEROL
Precipitation/Enzymatic-spectrophotometric
POLIVINYL SULPHATE-CHOLESTEROL OXIDASE/PEROXIDASE
Instrument:
SELECTRA-2
Reagent preparation
Samples
Sample preparation
Low density lipoproteins (LDL) in the sample precipitate with polivinyl sulphate. Reagent is ready to be used.
The supernatant contains low density lipoproteins (LDL). LDL cholesterol
concentration is calculated by substracting cholestrol values in serum from
supernatant values after being precipitated. The LDL cholesterol is then
spectrophotometrically measured by means of some coupled reactions.
Serum. Stable for 24 hours at 2-8C.
Precipitation:
1.- Pipette into labelled centrifuge tubes: 0.2 mL Sample + 0.1 mL Reagent B
2.- Mix thoroughly and let stand for 15 minutes at room temperature
3.- Centrifuge at a minimum of 4000 r.p.m. for 15 minutes
4.- Carefully collect the supernatant
Linearity: up to 500 mg/dL.

Interferences: Hemoglobin (1 g/L), bilirubin (10 mg/d/L) and acid ascorbic
(0.1 mmol/L) interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
LDL-Cholesterol
Endpoint
505 nm
mg/dL
0
0 mg/dL
500 mg/dL
(...)
3
1
*
(...)
No
LDL-Cholesterol
No
25 mL
300 L
300 L
6 L
3 L
11.5 min.
Prozone Check
Ref. Male Low
Ref. Male High
Ref. Female Low
Ref. Female High
No
150 mg/dL
150 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.150
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0206
MAGNESIUM
Spectrophotometric
CALMAGITE
Instrument:
SELECTRA-2
Reagent preparation
Samples
Magnesium in the sample reacts with calmagite in alkaline medium forming a Reagent is ready to be used.
coloured complex that can be measured by spectrophotometry. EGTA is
included in the reagent to remove calcium interference.
Serum, heparinized plasma.
Magnesium in serum or plasma is stable for 10 days at 2-8C.
Interferences: Hemoglobin (1.5 g/L), calcium (20 mg/dL) and bilirubin (20
mg/dL) do not interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
MAGNESIUM
Endpoint
505 nm
mg/dL
1
0.0 mg/dL
4.0 mg/dL
(...)
3
1
*
(...)
No
MAGNESIUM
No
25 mL
250 L
250 L
3 L
2 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
1.8 mg/dL
2.1 mg/dL
1.8 mg/dL
2.1 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.700
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0109
PHOSPHORUS
Spectrophotometric
PHOSPHOMOLYBDATE/UV
Instrument:
SELECTRA 2
Reagent preparation
Samples
Inorganic phosphorus in the sample reacts with molybdate in acid medium Working Reagent: Mix 35 mL Reagent A + 15 mL Reagent B. Mix thoroughly.
forming a phosphomolybdate complex that can be measured by Stable for 12 months at 15-30C.
spectrophotometry.
Phosphorus in serum or plasma is stable for 7 days at 2-8C. EDTA and fluoride may be used as anticoagulants.

Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Phosphorus
Endpoint
340 nm
mg/dL
2
0 mg/dL
20 mg/dL
(...)
3
1
*
(...)
No
Phosphorus
Yes
25 mL
300 L
300 L
3 L
2 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
2.70 mg/dL
4.50 mg/dL
2.70 mg/dL
4.50 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.500
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
PROTEIN
Spectrophotometric
BIURET
Instrument:
SELECTRA 2
Reagent preparation
Samples
Protein in the sample reacts with copper (II) ion in alkaline medium forming a Reagent is ready to be used.
Interferences: Hemoglobin (0.2 g/L) and bilirubin (15 mg/dL) interfere.

Moderate lipemia does not affect the results.
Linearity: Up to 150 g/L.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Protein
Endpoint
546 nm
g/L
1
0 g/L
150 g/L
(...)
3
1
*
(...)
No
Protein
No
25 mL
250 L
250 L
5 L
3 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
65 g/L
80 g/L
65 g/L
80 g/L
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.180
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
PROTEIN (URINE)
Spectrophotometric
PYROGALLOL RED
Instrument:
SELECTRA 2
Reagent preparation
Samples
Protein in the sample reacts with pyrogallol red and molybdate in acid medium Reagent is ready to be used.
forming a coloured complex that can be measured by spectrophotometry.
Urine, cerebrospinal fluid.
Linearity: Up to 4 g/L.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Protein-Urine
Endpoint
620 nm
g/L
2
0.00 g/L
4.00 g/L
(...)
3
1
*
(...)
No
Protein-Urine
No
25 mL
250 L
250 L
5 L
3 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0.05 g/L
0.14 g/L
0.05 g/L
0.14 g/L
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.180
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
TRIGLYCERIDES
GLYCEROL PHOSPHATE OXIDASE/PEROXIDASE
Instrument:
SELECTRA 2
Reagent preparation
Samples
Triglycerides in the sample originates, by means of some coupled reactions, a Reagent is ready to be used
Serum or plasma.

bilirubin (0.25 mmol/L) interfere. Lipemia does not affect results.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Triglycerides
Endpoint
505 nm
mg/dL
0
0 mg/dL
600 mg/dL
(...)
3
1
*
(...)
No
Triglycerides
No
25 mL
300 L
300 L
3 L
2 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
60 mg/dL
150 mg/dL
60 mg/dL
150 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.200
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
UREA/BUN
COLOR
Instrument:
SELECTRA 2
Reagent preparation
Samples
Urine in the sample originates, by means of some coupled reactions, a Reagent 1: Transfer the contents of one Reagent A2 vial into a Reagent A1
bottle. Mix thoroughly. Stable for 2 months at 2-8C.
Serum, plasma, urine. Stable for 7 days at 2-8C.
Heparin is recommended as anticoagulant.
Interferences: Ammonium salts of the anticoagulants interfere.

Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Incubation Time
Urea Color
Endpoint
620 nm
mg/dL
0
0 mg/dL
300 mg/dL
(...)
3
1
*
(...)
No
Urea Color
No
25 mL
220 L
220 L
2 L
1 L
25 mL
180 L
180 L
6.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
10 mg/dL
50 mg/dL
10 mg/dL
50 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.200
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
UREA/BUN
ULTRAVIOLET
Instrument:
SELECTRA 2
Reagent preparation
Samples
Urea in the sample consumes, by means of some coupled reactions, NADH Reagent 1: Use the Reagent A.
that can be measured by spectrophotometry.
Heparin is recommended as anticoagulant.
Interferences: Ammonium salts of the anticoagulants interfere.

Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Delay, min. Time
Urea
Twopoint
340 nm
mg/dL
0
0 U/L
300 U/L
(...)
3
1
*
(...)
No
Urea
No
25 mL
300 L
250 L
2 L
2 L
32, 70 sec.
Urea
No
25 mL
270 L
250 L
2 L
2 L
25 mL
30 L
30 L
No
No
24, 77 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
10 mg/dL
50 mg/dL
10 mg/dL
50 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.100
3.000
-0.100
2.000
0.100
Yes
(...)
(...)
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.100
3.000
-0.100
2.000
0.100
Yes
(...)
(...)

Version 0012
URIC ACID
URICASE/PEROXIDASE
Instrument:
SELECTRA 2
Reagent preparation
Samples
Uric acid in the sample originates, by means of some coupled reactions, a Reagent is ready to be used.
Magnesium in serum or plasma is stable for 10 days at 2-8C.

bilirubin (15 mg/dL) do not interfere. Lipemia may affect the results
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time
Uric acid
Endpoint
505 nm
mg/dL
1
0 mg/dL
25 mg/dL
(...)
3
1
*
(...)
No
Uric acid
No
25 mL
250 L
250 L
6 L
3 L
11.5 min.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
3.4 mg/dL
7.0 mg/dL
2.4 mg/dL
5.7 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
-0.100
3.000
-0.100
0.200
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
Yes
(...)
(...)

Version 0012
ANTI-STREPTOLYSIN O (ASO)
Turbidimetry
LATEX
Instrument:
SELECTRA 2
Reagent preparation
Samples
Anti-streptolysin O (ASO) causes agglutination of the latex particles coated Working Reagent: Pour the contents of a Latex vial into a Diluent bottle. Mix
with streptolysin O. The agglutination of the latex particles is proportional to the thoroughly.
ASO concentration and can be measured by turbidimetry.
Serum.
Stable for 7 days at 2-8 C.
Hemolyzed or lipemic samples are not suitable for testing..
Linearity: Up to 800 IU/mL.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Point one, two
ASO
Twopoint
546 nm
IU/mL
0
0 IU/mL
800 IU/mL
(...)
3
1
*
(...)
No
ASO
No
25 mL
300 L
300 L
3 L
2 L
12, 110 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 IU/mL
200 IU/mL
0 IU/mL
200 IU/mL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
0.900
0.150
Yes
(...)
(...)

Version 0012
COMPLEMENT COMPONENT C3
Turbidimetry
Instrument:
SELECTRA 2
Reagent preparation
Samples
Complement component C3 precipitates in the presence of anti-human C3 Reagent 1: Use the Reagent A.
antibodies. The originated turbidity is proportional to the C3 concentration and Reagent 2: Use the Reagent B.
can be measured by turbidimetry.
Serum or plasma treated with heparin or EDTA.
Stable 2 days at 2-8 C.
Hemolyzed or lipemic samples are not suitable for testing.
The measurement interval depends on concentration of the highest

standard.
Due to the zone effect, falsely low values will be obtained when C3 is
present in the sample at a concentration higher than 600 mg/dL.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Point one, two
Complement C3
Twopoint
340 nm
mg/dL
0
0 mg/dL
600 mg/dL
(...)
2
5
*
(...)
No
Complement C3
No
25 mL
220 L
220 L
3 L
3 L
5 mL
50 L
50 L
No
No
-3, 236 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
90 mg/dL
180 mg/dL
90 mg/dL
180 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
3.000
0.000
Yes
(...)
(...)
1.00

Version 0109
COMPLEMENT COMPONENT C4
Turbidimetry
Instrument:
SELECTRA 2
Reagent preparation
Samples
Complement component C4 precipitates in the presence of anti-human C4 Reagent 1: Use the Reagent A.
antibodies. The originated turbidity is proportional to the C4 concentration and Reagent 2: Use the Reagent B.
can be measured by turbidimetry.
Stable 2 days at 2-8 C.

standard.
Due to the zone effect, falsely low values will be obtained when C4 is
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Point one, two
Complement C4
Twopoint
340 nm
mg/dL
0
0 mg/dL
150 mg/dL
(...)
2
5
*
(...)
No
Complement C4
No
25 mL
220 L
220 L
9 L
4 L
5 mL
50 L
50 L
No
No
-3, 236 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
10 mg/dL
40 mg/dL
10 mg/dL
40 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
3.000
0.000
Yes
(...)
(...)
1.00

Version 0102
C-REACTIVE PROTEIN (CRP)
Turbidimetry
LATEX
Instrument:
SELECTRA 2
Reagent preparation
Samples
Serum C-reactive protein (CRP) causes agglutination of the latex particles Working Reagent: Pour the contents of a Latex vial into a Diluent bottle. Mix
coated with anti-human C-reactive protein. The agglutination of the latex thoroughly.
particles is proportional to the CRP concentration and can be measured by Stable for 20 days at 2-8C.
turbidimetry.
Serum.
Linearity: Up to 150 mg/L.

Interferences: Rheumatoid factors, up to 200 IU/mL, do not interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Point one, two
CRP
Twopoint
546 nm
mg/L
0
0 mg/L
150 mg/L
(...)
3
1
*
(...)
No
CRP
No
25 mL
398 L
398 L
2 L
1 L
12, 110 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 mg/L
6 mg/L
0 mg/L
6 mg/L
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
0.900
2.000
Yes
(...)
(...)

Version 0012
C-REACTIVE PROTEIN-hs (CRP-hs)

Turbidimetry
LATEX-HIGH SENSITIVITY
Instrument:
SELECTRA 2
Reagent preparation
Samples
Serum C-reactive protein (CRP) causes agglutination of the latex particles Working Reagent: Pour the contents of a Reagent B vial into a Reagent A
coated with anti-human C-reactive protein. The agglutination of the latex bottle. Mix thoroughly.
particles is proportional to the CRP concentration and can be measured by Stable for 20 days at 2-8C.
turbidimetry.
Serum.
Measurement: 0.06-15 mg/L.

Interferences: Hemoglobin (10 g/L) and lipemia (triglycerides 10 g/L) do
not interfere. Bilirubin (>10 mg/dL) and rheumatoid factors (>75 IU/mL)
may interfere.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Point one, two
CRP-hs
Twopoint
546 nm
mg/L
1
0 mg/L
15 mg/L
(...)
3
1
*
(...)
No
CRP-hs
No
25 mL
225 L
225 L
3 L
2 L
12, 304 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 mg/L
5 mg/L
0 mg/L
5 mg/L
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
1.000
1.700
2.000
Yes
(...)
(...)

Version 0203
IMMUNOGLOBULIN A (IgA)
Turbidimetry
Instrument:
SELECTRA 2
Reagent preparation
Samples
Immunoglobulin A precipitates in the presence of anti-human immunoglobulin Reagent 1: Use the Reagent A.
A antibodies. The originated turbidity is proportional to the immunoglobulin A Reagent 2: Use the Reagent B.
concentration and can be measured by turbidimetry.
Use the Working Reagent if MONO MODE.

standard.
Due to the zone effect, falsely low values will be obtained when IgA is
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Point one, two
Ig A
Twopoint
620 nm
mg/dL
0
0 mg/dL
1300 mg/dL
(...)
2
5
*
(...)
No
Ig A
No
25 mL
220 L
220 L
3 L
2 L
5 mL
50 L
50 L
No
No
-3, 236 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
70 mg/dL
400 mg/dL
70 mg/dL
400 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
3.000
0.000
Yes
(...)
(...)
1.00

Version 0110
IMMUNOGLOBULIN G (IgG)
Turbidimetry
Instrument:
SELECTRA 2
Reagent preparation
Samples
Immunoglobulin G precipitates in the presence of anti-human immunoglobulin Reagent 1: Use the Reagent A.
G antibodies. The originated turbidity is proportional to the immunoglobulin G Reagent 2: Use the Reagent B.
Use the Working Reagent if MONO MODE.

standard.
Due to the zone effect, falsely low values will be obtained when IgG is
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Point one, two
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Point one, two
Ig G
Twopoint
620 nm
mg/dL
0
0 mg/dL
8000 mg/dL
(...)
2
5
*
(...)
No
Ig G
No
25 mL
220 L
220 L
2 L
2 L
5 mL
50 L
50 L
No
No
-3, 236 sec.
Ig G
No
25 mL
250 L
250 L
5 L
5 L
12, 70 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
700 mg/dL
1600 mg/dL
700 mg/dL
1600 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
3.000
0.000
Yes
(...)
(...)
1.00
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
0.000
3.000
0.000
3.000
0.100
Yes
(...)

Version 0109
IMMUNOGLOBULIN M (IgM)
Turbidimetry
Instrument:
SELECTRA 2
Reagent preparation
Samples
Immunoglobulin M precipitates in the presence of anti-human immunoglobulin Reagent 1: Use the Reagent A.
M antibodies. The originated turbidity is proportional to the immunoglobulin M Reagent 2: Use the Reagent B.

standard.
Due to the zone effect, falsely low values will be obtained when IgM is
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Point one, two
Ig M
Twopoint
340 nm
mg/dL
0
0 mg/dL
600 mg/dL
(...)
2
5
*
(...)
No
Ig M
No
25 mL
220 L
220 L
5 L
3 L
5 mL
50 L
50 L
No
No
-3, 236 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
40 mg/dL
230 mg/dL
40 mg/dL
230 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
3.000
0.000
Yes
(...)
(...)
1.00

Version 0102
ALBUMIN (URINE)
Turbidimetry
LATEX
Instrument:
SELECTRA 2
Reagent preparation
Samples
Albumin in the urine sample causes agglutination of the latex particles coated Working Reagent: Pour the contents of a Latex vial into a Diluent bottle. Mix
with anti-human albumin. The agglutination of the particles is proportional to thoroughly. Stable for 8 hours at 2-8C.
the albumin concentration and can be measured by turbidimetry.
Urine.
Urine should be centrifugated befor analysis.
Linearity: Up to 130 mg/L.

The zone effect will cause to obtain falsely low values when albumin is
present in the sample at a concentration higher than 1000 mg/L.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Point one, two
Albumin (Urine)
Twopoint
546 nm
mg/L
0
0 mg/L
130 mg/L
(...)
3
1
*
(...)
No
Albumin (Urine)
No
25 mL
300 L
300 L
2 L
1 L
12, 110 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 mg/L
15 mg/L
0 mg/L
15 mg/L
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
0.900
0.200
Yes
(...)
(...)

Version 0012
RHEUMATOID FACTORS (RF)
Turbidimetry
LATEX
Linear calibration
Instrument:
SELECTRA 2
Reagent preparation
Samples
Rheumatoif factors (RF) cause agglutination of the latex particles coated with Reagent 1: Use the Diluent.
human gamma-globulin. The agglutination of the latex particles is proportional Reagent 2: Use the Latex.
to the RF concentration and can be measured by turbidimetry.
Serum.
Linearity: Up to 120 IU/mL.

This method has not zone effect up to 800 IU/mL.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Point one, two
RF
Twopoint
620 nm
IU/mL
1
0 IU/mL
120 IU/mL
(...)
2
1
*
(...)
No
RF
No
25 mL
350 L
270 L
3 L
2 L
25 mL
40 L
30 L
No
No
-3, 120 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 IU/mL
20 IU/mL
0 IU/mL
20 IU/mL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
3.000
2.000
Yes
(...)
(...)

Version 0012
RHEUMATOID FACTORS (RF)
Turbidimetry
LATEX
Non linear calibration
Instrument:
SELECTRA 2
Reagent preparation
Samples
Rheumatoif factors (RF) cause agglutination of the latex particles coated with Reagent 1: Use the Diluent.
human gamma-globulin. The agglutination of the latex particles is proportional Reagent 2: Use the Latex.
to the RF concentration and can be measured by turbidimetry.
Serum.
Linearity: Up to highest value of standard.

This method has not zone effect up to 800 IU/mL.
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Point one, two
RF
Twopoint
620 nm
IU/mL
1
0 IU/mL
... IU/mL
(...)
2
5
*
(...)
No
RF
No
25 mL
350 L
270 L
3 L
2 L
25 mL
40 L
30 L
No
No
-3, 120 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
0 IU/mL
20 IU/mL
0 IU/mL
20 IU/mL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
3.000
2.000
Yes
(...)
(...)

Version 0106
TRANSFERRIN
Turbidimetry
Instrument:
SELECTRA 2
Reagent preparation
Samples
Transferrin precipitates in the presence of anti-human transferrin antibodies. Reagent 1: Use the Reagent A.
The originated turbidity is proportional to the transferrin concentration and can Reagent 2: Use the Reagent B.
be measured by turbidimetry.
Interferences: Hemoglobin (10 g/L), bilirubin (20 mg/dL) and Rheumatoid

factors (300 UI/mL) do not interfere. Lipemia does not affect the results (5
g/L).
Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
DUAL MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
R2 Bottle
Normal Volume
Rerun Volume
Predilution
Slope Blank
Point one, two
Transferrin
Twopoint
340 nm
mg/dL
0
0 mg/dL
... mg/dL
(...)
2
5
*
(...)
No
Transferrin
No
25 mL
220 L
220 L
2 L
4 L
5 mL
50 L
50 L
No
No
-3, 236 sec.
Prozone Check
No
Ref. Male Low

Ref. Male High
Ref. Female Low
Ref. Female High
200 mg/dL
360 mg/dL
200 mg/dL
360 mg/dL
Correlation Factor
Correlation Offset
1.000
0.000
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor
-0.050
3.000
-0.050
3.000
0.000
Yes
(...)
(...)
1.00

Version 0105

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ACID PHOSPHATASE (ACP)

Acid phosphatase (ACP) catalyzes in acid medium the hydrolysis of the

Linearity: up to 150 U/L.

Ref. Male Low

If the test is run without calibration enter

Principle of the method

Ref. Male Low

... Data entered by the operator

ALKALINE PHOSPHATASE (ALP)

Principle of the method

Alkaline phosphatase (ALP) catalyzes in alkaline medium the transfer of the

Working Reagent: Dissolve the powder of a Reagent B vial with 20 mL of the

Linearity: up to 690 U/L.

Ref. Male Low

If the test is run without calibration enter

ALKALINE PHOSPHATASE (ALP)

Principle of the method

Alkaline phosphatase (ALP) catalyzes in alkaline medium the transfer of the

Working Reagent: Dissolve the powder of a Reagent B vial with 20 mL of the

Linearity: up to 1200 U/L.

Ref. Male Low

If the test is run without calibration enter

ALANINE AMINOTRANSFERASE (ALT)

Principle of the method

Alanine aminotransferase (ALT or GPT) catalyzes the transfer of the amino

Ref. Male Low

If the test is run without calibration enter

Principle of the method

-Amylase catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-maltotrioside Reagent is ready to be used.

Linearity: up to 1300 U/L (serum) or 2600 U/L (urine).

Ref. Male Low

If the test is run without calibration enter

Principle of the method

Serum, plasma, urine.

Linearity: up to 1300 U/L (serum) or 2600 U/L (urine).

Ref. Male Low

If the test is run without calibration enter

ASPARTATE AMINOTRANSFERASE (AST)

Principle of the method

Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino

Ref. Male Low

If the test is run without calibration enter

Principle of the method

Ref. Male Low

... Data entered by the operator

Principle of the method

Ref. Male Low

... Data entered by the operator

Principle of the method

Ref. Male Low

... Data entered by the operator

Principle of the method

Cholinesterase (CHE) catalyzes the hydrolysis of benzoylcholine to choline and

Working Reagent: Reconstitute the contents of a Reagent B vial with 3 mL (if

Linearity: up to 6500 U/L.

Ref. Male Low

If the test is run without calibration enter

Principle of the method

Interferences: Hemoglobin (3 g/L), ascorbic acid (0.3 mmol/L) and

Ref. Male Low

... Data entered by the operator

CREATINE KINASE (CK)

Principle of the method