Professional Documents
Culture Documents
Lifestyle,
Health and
Risk
Explain the importance of water as a solvent in transport,
including its dipole nature. (2).
June 2010: Water is described as a dipolar molecule because it has a:
Positively charged hydrogen end and a negatively charged oxygen end
Consists of () glucose
June 2012: Carbohydrates are important components of our diets. Distinguish between the
structures of each monosaccharides and disaccharides and amylose and amylopectin of
carbohydrate molecules.
Monosaccharides and disaccharides:
Idea that a monosaccharide consists of one sugar / named sugar / unit whereas a disaccharide
consists of two sugar units
Idea that disaccharide has a glycosidic bond whereas monosaccharide does not
General formula for a monosaccharide is CnH2nOn whereas formula for disaccharide is CnH2n2On-1
Amylose and amylopectin:
glucose
Glycosidic bonds / links
Amylose and amylopectin
Amylose has 1- 4 glycosidic bonds / links
AND amylopectin has 1- 4 and 1- 6 glycosidic bonds
Amylose is spiralled / coiled
Amylopectin is branched
Compact molecule
January 2013: Explain the meaning of the following terms: catalyst and hydrolysis.
Catalyst:
January 2013: Bread contains a high proportion of starch. If bread is chewed for a long
period of time it begins to taste sweet. Suggest why bread tastes sweet after chewing for a
long period of time.
Idea that maltose / disaccharide / glucose / monosaccharide is produced and so tastes sweet.
Both
in
correctly
hexose molecules
disaccharide
drawn
Indication
that water is
Glycosidic
bond correctly
formed
drawn
June 2011: Name the chemical reaction that joins the galactose and glucose molecules
together.
Condensation reaction (polymerisation)
June 2011: Name the bond that joins the galactose and glucose molecules together.
1-4 glycosidic bond
lipids.(5)
January 2011: Show the synthesis of a triglyceride by a condensation reaction.
Aorta
tick
tick
Vena cava
cross (X)
cross (X)
Pulmonary artery
tick
cross (X)
Pulmonary ven
cross (X)
tick
January 2010: Suggest the advantages that the human circulatory system has compared
with that of a fish.
January 2010: The heart of an insect is a long tube. It pumps blood into the body cavity so
that blood surrounds the cells. The blood then passes back into the heart from the body
cavity. Suggest why the insect does not need blood vessels to transport its blood around
the body.
June 2010: Read through the following passage about the heart and its major blood
vessels, added on the dotted lines are the most appropriate word or words to complete the
passage.
The mammalian heart consists of four chambers, two upper chambers called
....................atria........................... and two lower chambers called ventricles.
The ..................aorta............................. carries oxygenated blood away from the
.....................left.......................... ventricle to the cells of the body and the pulmonary
..................artery............................. carries deoxygenated blood to the lungs. The vena
cava............................. returns deoxygenated blood back to the heart from the body.
January 2011: Give one reason why many animals have a circulatory system.
June 2012: Many animals, such as mammals, have a heart and circulation. This helps them
to meet their requirements by overcoming the limitations of diffusion. Describe the
structure of the mammalian heart.
June 2012: Using your own knowledge, explain the importance of the heart and circulation
to a giraffe.
Idea that the heart has to pump blood a long way around the body of the giraffe
Idea of oxygen / glucose needed as high metabolic rate / high rate of respiration
June 2012: Suggest why there are changes to the available energy (ATP) in the heart muscle
cells following the loss of blood flow.
June 2009: During the cardiac cycle, the pressure in the right ventricle rises to a maximum
of about 3.3 kPa. Suggest reasons for the difference between this pressure and the
maximum pressure in the left ventricle.
January 2013: Read through the following passage on the cardiac cycle, on the dotted
lines the most appropriate word or words have been written in to complete the passage.
The cardiac cycle consists of three stages: atrial systole, ventricular systole and
....................diastole.................... .
During atrial systole, the ..........atria............. contract and the
..........................ventricles............ are relaxed. The ..........atrioventricular............ valves are open.
During ventricular systole, the .semilunar valves.................... open as oxygenated
blood is forced out of the heart through the aorta to the body and through the pulmonary
................artery...................... to the lungs.
Capillary
No
Yes
Yes
Vein
Yes
No
Yes
January 2011: Semilunar valves and elastic fibres are found in the aorta.
For each of these structures, describe its location in the aorta and explain its function.
Semilunar valves:
Idea that there is a thick wall / lots of collagen / thick layers / thick tunica media (ALLOW idea of
folded wall) idea that it needs to avoid rupture / to withstand high pressure (IGNORE damage
alone ALLOW stretch to accommodate more blood)
Elastic / muscular/ layer / fibres / wall controlS the flow of blood / maintain blood pressure /
elastic recoil (ALLOW to squeeze blood along smooth endothelial wall to reduce friction /
resistance
Semi lunar valve present to prevent backflow of blood into the heart during diastole
Large lumen (IGNORE narrow lumen) idea of accommodating large volumes of blood
Branches to supply blood to different parts of the body including coronary arteries
January 2013: Describe two differences between the structure of a capillary and the
structure of a vein.
Idea that heart rate of daphnia determined in white tea only / known caffeine concentration
January 2010: Give one ethical reason for the use of invertebrates and one ethical reason
against the use of invertebrates in experiments of this type.
For:
Daphnia are very simple organisms / Daphnia have basic nervous system
Against:
Use of (any) animal is wrong / how can we be sure what the Daphnia can feel / ref. to possibility
that the Daphnia could die
January 2013: An investigation was carried out to study the effect of caffeine on the heart
rate of a chicken embryo. The heart from a chicken embryo was removed and placed in a
glucose solution. The heart rate was determined and recorded as the base heart rate. The
experiment was repeated using glucose solutions containing five different concentrations
of caffeine. The heart rate was determined and recorded as a percentage of the base heart
rate for each solution. Suggest why glucose was included in the solutions.
January 2013: Suggest how the caffeine solutions were prepared to obtain valid results.
Glucose / pH at same concentration / volume / value.
January 2013: State how these results could be made more reliable.
Reference to replication procedure.
January 2013: Describe how this investigation could be carried out using Daphnia instead
of chicken embryos.
Details of method to limit movement of Daphnia e.g. use of cotton wool IGNORE cavity slide
Reference to determining base heart rate in absence of caffeine ALLOW measure heart rate in
0% caffeine NOT distilled water
Repeats / replicates
Named control variable e.g. source / size / age / type / of Daphnia, temperature, pH
January 2013: Suggest one ethical issue in the use of chicken embryos in this investigation.
Chicken embryo is a vertebrate / feels pain / will die / cannot give consent
June 2010: Using your own knowledge of the blood clotting process, suggest why frequent
cocaine use could increase the risk of a blood clot forming with the knowledge that the von
Willebrand factor is involved in platelets sticking to each other and platelets sticking to the
endothelial lining of blood vessels .
Idea that von Willebrand factor results in platelets sticking to the endothelium / each other.
Reference to release of thromboplastin from platelets.
If the blood is flowing slower then there is an increased chance of blood clotting
June 2013:
June 2009: One cause of coronary heart disease is atherosclerosis. Describe how
atherosclerosis develops.
January 2011: Cardiovascular diseases are very common in the Western World. (a) Many
cardiovascular diseases result from atherosclerosis.
Atherosclerosis usually results from the formation of plaques inside:
Arteries
The plaques begin to form after damage to:
Endothelial cells
These cells may be damaged due to:
Blood flowing quickly under high pressure
The plaque consists of:
Fatty deposits
The presence of a plaque in the vessels supplying blood to the brain could result in:
A stroke
January 2012: Cardiovascular disease (CVD) is responsible for many deaths. One cause of
CVD is atherosclerosis. Describe how atherosclerosis develops.
Genetic
Diet qualified
Increasing age
Being male
High blood pressure
Smoking,
Inactivity / lack of exercise
June 2011: Risk calculators can be used to estimate the probability that a person will
develop CVD. Many of these calculators start by asking for the age and gender of the
person using them. Explain why information about age and gender is important in
estimating the risk of developing CVD.
Age effect qualified e.g. older increases risk, arteries become less elastic / more easily damaged
/ blood pressure increases with increase in age
Gender effect qualified e.g. women less likely to develop CVD than men / oestrogen offers some
protection to women against CVD pre menopause.
January 2012: Give two factors, other than genetic factors, that increase the risk of
developing CVD.
More saturated fat / more cholesterol / more salt /obesity / more alcohol / more age / male / postmenopausal women / high blood pressure / smoking / diabetes / less activity / stress.
January 2013: State two factors, other than obesity, high blood pressure and high blood
cholesterol, that increase the risk of CVD.
Any two from:
Being male
Increase in age
Lack of exercise / inactivity
Smoking
Genetics
High alcohol consumption high salt diet
High saturated fat intake stress
Diabetes
irregular heart rate / chest pain / hives / rash / dehydration / reduced circulation effects / low
potassium / blurred vision.
January 2012: Trials have shown that plant statin therapy is more effective in 719 Arg
carriers than in non-carriers of this allele. Describe the risks of using plant statins to treat
CVD.
June 2012: Give the name of the type of drug that is used to treat high blood pressure.
Antihypertensives / antihypertensive drug / beta blockers / diuretics / ACE inhibitors / calcium ion
channel blockers / vasodilators.
June 2012: Explain why many patients, who are over 80 and have high blood pressure, are
routinely prescribed with drugs such as antihypertensives.
High blood pressure can cause / increases risk of CVD / correctly named complication /
description
People more aware of the dangers / better health education / appropriate named example
Less stress
Better / more screening
Better treatments
More exercise being taken
Changed diet / less obesity
Less alcohol intake
Decrease in smoking
Change in population genetics
Idea that the woman could reduce her energy intake weight / BMI decreases if her energy
expenditure greater than intake.
Diet should have reduced cholesterol levels cholesterol has been associated with high blood
pressure / atherosclerosis.
Diet should have reduced saturated fat reduces blood cholesterol / LDL
Idea that the woman could increase the amount of exercise she took weight decreases if energy
expenditure is greater than her intake / exercise helps maintain a healthy heart / reduces blood
pressure.
Idea that if the woman smoked she should reduce it smoking reduces oxygen uptake /
increases stickiness of platelets / increases blood pressure / increases risk of atheroma.
Idea that diet should have reduced salt high salt associated with high blood pressure.
Idea of moderate alcohol intake high alcohol associated with high blood pressure.
June 2011: Give three other ways apart from lowering blood cholesterol levels and lowering
blood pressure in which the risk of CVD may be reduced.
January 2013: Suggest two reasons for the overall decrease in high blood cholesterol as a
risk factor.
June 2009: Describe how an investigation could be carried out to compare the effect of
storage time on the vitamin C content of the Paranastate camu-camu fruit with those from
the Amazon region.
Reference to DCPIP
June 2011: It has been suggested that cooking food in a microwave oven does not reduce
the nutrient content of foods by as much as cooking in boiling water. A student wanted to
test this idea on the vitamin C content of carrots. Describe an investigation that the student
could carry out to compare these two methods of cooking on the vitamin C content of
carrots.
Idea that some carrots need to be boiled in water and some cooked in microwave
Reference to control of appropriate variable
Reference to juice / cooking water being used
Reference to DCPIP
Reference to titration / description of titration of juice
Colour change of DCPIP e.g. from blue to colourless / pink as juice added / until stays blue as
DCPIP added
Reference to repeats
June 2012: Explain why a diet consisting of a high proportion of carbohydrates could lead
to obesity.
Idea that a correlation is a relationship between two factors / when one factor changes another
factor changes.
Saline
Water
Sugar tablet
Empty capsule
June 2010: Suggest why this study had to run for a number of years.
Topic 2:
Genes and
health
Phospholipids;
Phosphate head
Two fatty acid tails
Reference to location of glycerol
Correct reference to ester bonds
June 2010: Explain how the properties of a phospholipid contribute to the structure of the
cell membrane.
June 2010: Describe how the results of an investigation showing that proteins from two
different cells have intermingled in one cell after fusion can be explained by the fluid
mosaic model.
Proteins
Credit details of proteins e.g. description of channel/carrier protein structure or position
intrinsic, extrinsic or transmembrane
June 2011: A student wanted to sweeten some strawberries, so she sprinkled some sugar
on top of them, one hour before eating them. The student noticed that the sugar that she
had sprinkled on them was no longer visible and that there was some juice at the bottom of
the bowl. Using your knowledge of cell transport mechanisms and the properties of water,
explain how the juice is formed from the water that came from the fruit.
June 2011: Molecules are transported into and out of cells by several mechanisms.
Read through the following passage that describes some of these mechanisms, the the
most appropriate word or words have been added on the dotted lines to complete the
passage.
Some molecules move across a cell surface membrane by passing down a
concentration gradient, through the phospholipid bilayer. The movement of some polar molecules
across the membrane involves carrier and channel ............................protein.......................
molecules. When this movement occurs down a concentration gradient, the process is called
..facilitated diffusion ........................... and when it occurs against a concentration
gradient the process is called ..active transport................................. .
Energy in the form of ........................................ATP......................................... is used in the
movement of molecules against a concentration gradient
June 2012: Suggest how oxygen passes from the cell membrane into the centre of an
amoeba.
Description of how permeability can be assessed e.g. use of colorimeter, standard solutions.
Reference to repeats / replicate.
June 2010: Explain why it is necessary to measure the initial rate of reaction when
investigating the effect of enzyme concentration on the rate of reaction.
Idea that there should be enough substrate molecules to saturate the enzyme
To ensure that substrate is not a limiting factor
Fastest / highest rate / decreases after initial rate
As reaction proceeds substrate concentration decreases
As substrate gets used up by enzyme / in reaction
Substrate concentration should be constant in each test
June 2010: In this investigation, the substrate concentration was a factor that was kept
constant. Suggest two other factors that should be kept constant. For each factor, state how
it can be kept constant.
pH buffer
Temperature water bath (not room temperature)
Time of reaction stopwatch
Volume of enzyme / substrate (not amount) measuring cylinder / pipette
Type of enzyme same batch of enzym
June 2012: The action of lipase (enzyme) can be investigated using a triglyceride as the
substrate. Describe an experiment, using lipase and a triglyceride, that could be carried out
to collect data to plot a graph.
January 2011: Using your knowledge of enzymes, explain the importance of the primary
structure of an enzyme to its function.
Reference to position of amino acids determines position of bonds / correctly named bond
Correct reference to two cys (aminoacids) form bonds
Idea that shape / position / of active site is determined by position of amino acids
Reference to shape of active site being correct to bind to substrate
Reference to amino acids / R groups involved in chemical reaction
Reference to globular / soluble / enzyme molecules being relatively short / small / made up of
relatively few amino acids.
Reference to globular / soluble proteins / enzyme having relatively high number of polar / small
amino acids / R groups.
January 2012: Proteins, such as enzymes, are important molecules found in all living
organisms. Read through the following passage on the primary structure of proteins, where
the most appropriate word or words have been written on the dotted lines to complete the
passage.
Proteins are made of monomers called amino acids............... . These monomers are
joined together by .peptide .......................... bonds, formed during
.condensation (polymerisation) reactions. Each monomer of a protein consists of a
central carbon atom attached to a hydrogen atom, an R group, an ............amine......... group and
a ..carboxyl (carboxylic acid). group. The sequence of monomers
determines the primary structure of the protein.
January 2013: Lipoproteins are composed of phospholipids, cholesterol and proteins. (i)
Proteins are made up of amino acids. Describe how amino acids join together to form the
three-dimensional structure of a protein.
Disulfide bridges / hydrogen / H bonds / ionic bonds / Van der Waals forces ;
Between the R groups
Globular
Reference to active site
Reference to specific shape of active site
January 2012: Explain how the primary structure of an enzyme determines its threedimensional (tertiary) structure and its properties.
Idea that there should be enough substrate molecules to saturate the enzyme
To ensure that substrate is not a limiting factor
Fastest / highest rate / decreases after initial rate
As reaction proceeds substrate concentration decreases
As substrate gets used up by enzyme / in reaction
Substrate concentration should be constant in each test
June 2010: In this investigation, the substrate concentration was a factor that was kept
constant. Suggest two other factors that should be kept constant. For each factor, state how
it can be kept constant.
pH buffer
Temperature water bath (not room temperature)
Time of reaction stopwatch
Volume of enzyme / substrate (not amount) measuring cylinder / pipette
Type of enzyme same batch of enzyme
January 2012: Describe how an inverted measuring cylinder under water could be used to
compare enzyme activity in two different types of mussel.
June 2011: In the late 1950s, Meselson and Stahl performed some important experiments.
These experiments provided evidence to support the idea that new DNA was synthesised by
semi-conservative replication. Name an enzyme involved in DNA replication.
DNA polymerase
DNA helicase
DNA ligase
June 2012: Describe the role of each mRNA and tRNA in protein synthesis.
mRNA:
Idea of mRNA being a copy of the antisense DNA strand / template DNA strand / coding DNA
strand / gene / allele / part of DNA
Idea that mRNA made up of codons / codes for specific amino acids / code for amino acid
sequence
Idea of mRNA being taken into the cytoplasm / to the ribosomes / out of the nucleus
Used in translation
Binds to ribosome
tRNA:
Idea that DNA molecule unwinds / unzips / uncoils / DNA strands separate
Free RNA mono nucleotides line up against / attach to one strand / template / antisense strand
Ref to complementary base pairing between DNA and mononucleotides
Ref to formation of phosphodiester bonds
Ref to condensation reaction
Correct name of enzyme involved
Idea that mRNA detaches from the DNA
January 2013: Describe two ways in which the structure of a tRNA molecule differs from the
structure of a mRNA molecule.
parents, this person will show symptoms of anaemia and will require blood transfusions.
Symptoms of anaemia include tiredness and breathlessness. Using the information given
above and your knowledge of gene mutation, suggest why a person with beta thalassaemia
has symptoms of anaemia.
Idea that fewer albino squirrels survive/ squirrels may not breed so frequently.
A suitable reason given (e.g.more predation, less camouflage)
Idea of frequency of albinism allele in squirrel (population) is lower / chances of two squirrels
with the allele less likely to mate.
Idea that both of these alleles need to be present in order for the recessive phenotype to be
expressed
January 2013: Cystic fibrosis and albinism are examples of recessive genetic disorders.
Tay-Sachs disease is another example of a recessive genetic disorder. Explain the meaning
of the term recessive genetic disorder.
Recessive genetic disorder:
June 2010: Suggest why people with cystic fibrosis are more likely to suffer from lung
infections than people without cystic fibrosis.
June 2011: Cystic fibrosis is a genetic disease that can affect many body systems,
including the digestive system. In a carrier of this disorder, preimplantation genetic
diagnosis can be used to detect the presence of an allele for cystic fibrosis. Explain how
cystic fibrosis affects the digestive system.
June 2012: Cystic fibrosis and albinism are examples of recessive genetic disorders.
Krabbe disease is another example of a recessive genetic disorder.
Krabbe disease is caused by mutations in the GALC gene, resulting in a deficiency of an
enzyme called galactocerebrosidase. Suggest how a mutation in the GALC gene could
result in a change in the enzyme galactocerebrosidase.
Idea of a gene being a sequence of bases that code for the sequence of amino acids in the
Suggest why a gene coding for an endorphin was used in this investigation.
January 2012: Gene therapy has the potential to treat some genetic disorders. Explain why
gene therapy has the potential to treat some genetic disorders.
January 2012: Suggest how patients with cystic fibrosis could be treated using gene
therapy.
Reference to using alleles / genes coding for the CFTR protein / channel
Reference to introducing the alleles / genes into the cells
Of the lungs / pancreas / reproductive tracts
That produce mucus
Using a vector / named vector
Credit suitable delivery mechanism e.g. nebuliser, injection
Idea that treatment needs to be repeated due to cell replacement
January 2013: Tay-Sachs disease is caused by a gene mutation that results in the build up
of lipid in the brain. It is hoped that gene therapy will be able to treat this disease in the
future.Sheep can also suffer from Tay-Sachs disease. Investigations have found that gene
therapy increases the life span of these animals. Suggest how these gene therapy
investigations could have been carried out.
Fetal /embryonic cells present in amniotic fluid /fetal / embryonic cells needed
DNA can be analysed
June 2009: Describe one benefit and one risk, to a pregnant woman, of prenatal testing:
Benefit:
January 2010: Cystic fibrosis is a life-threatening condition that can affect many different
parts of the body. It is a recessive genetic trait. Genetic screening can be used to test for
the presence of recessive alleles. A person found to possess a recessive allele is called a
carrier. Suggest why cells from mouth swabs or blood samples are used rather than
gametes.
January 2010: Explain why it is necessary to test for several different recessive alleles in
the screening for cystic fibrosis.
Cystic fibrosis results from one of a number of possible mutations of this gene
Idea that testing for only one will miss other recessive alleles
January 2010: If neither partner is a carrier then it is considered that the chance of having a
child with cystic fibrosis is low. Explain why the probability of having a child with cystic
fibrosis is low and not zero.
June 2011: Explain how preimplantation genetic diagnosis is performed to detect cystic
fibrosis.
Amniocentesis
Chorionic villus sampling
Denying them the opportunity to live / fetus should be allowed to live / fetus has a right to live
June 2011: Discuss either one ethical issue or one social issue relating to the use of
preimplantation genetic diagnosis.
Any of the following paired points:
Or:
If abnormality found
Consequence of abnormality found e.g. abortion, comment on possible problems with future
employment / insurance / what constitutes a serious condition
Or: