WWW - Emtech.upm - Edu.my Document EMTech Calendar

Celebrating the International Year of
Crystallography
2014 CALENDAR
Enzyme and Microbial Technology Research Centre
Enzyme and Microbial Technology Research

Centre (EMTECH),
Faculty of Biotechnology and Biomolecular Sciences,
Universiti Putra Malaysia, 43400 Serdang, Selangor,
MALAYSIA.
Tel: +603-8947 1416 Fax: +603-8946 7590
UPM Academic Calendar

FIRST SEMESTER
Classes
9 September 2013 - 3 November 2013
8 weeks
# Mid-semester Holidays
4 November - 10 November 2013
1 week
* Classes
11 November 2013 - 22 December 2013
6 weeks
# Study Break
23 December 2013 - 29 December 2013
1 week
Final Examination
30 December 2013 - 12 January 2014
2 weeks
- Breaks Between Semester
13 January 2014 - 16 February 2014
5 weeks
## Classes
17 February 2014 - 6 April 2014
7 weeks
Mid-semester Holidays
7 April 2014 - 13 April 2014
1 week
Classes
14 April 2014 - 1 June 2014
7 weeks
Study Break
2 June 2014 - 8 June 2014
1 week
Final Examination
9 June 2014 - 22 June 2014
2 weeks
*** Final Semester Break
13 January 2014 - 16 February 2014
10 weeks
Classes
23 June 2014 - 10 August 2014
7 weeks
Final Examination
11 August 2014 - 18 August 2014
1 weeks
SYMBOL
DATE
DAY
** Deepavali
2 November 2013
Saturday
# Christmas
25 December 2013
Wednesday
## Chinese New Year
31 January - 1February 2014
Friday & Saturday
*** Hari Raya Puasa
28 - 29 July 2014
Monday & Tuesday
SECOND SEMESTER
THIRD SEMESTER (SPECIAL)
EMTECH Activity Calendar

Workshop on Manuscript Writing
March
EMTECH Colloquium 2014
* May/June
Workshop on Protein Crystallography
* November/December
* To be confirmed. Subject to change
EMTECH also offers service of renting equipments. For a full list of equipments,
please contact +603 8947 1416 for further information.
From left to right: Freeze dryer, spray dryer, bioreactor (7.5 L)
From left to right: YSI 2700 Select Biochemistry Analyzer, circular dichroism (CD), gel documentation
2014 Public Holidays

1 January 2014 (Wednesday)
New Year Celebration
All states except Johor, Kedah, Kelantan, Perlis &

Terengganu
14 January 2014 (Tuesday)
Prophet Muhammads Birthday
All states
17 January 2014 (Friday)
Thaipusam
Kuala Lumpur, Putrajaya, Johor, N. Sembilan,

Perak, Penang & Selangor
31 January 2014 (Friday)
Chinese New Year (Day 1)
All states except Kelantan & Terengganu
1 February 2014 (Saturday)
Chinese New Year (Day 2)
1 May 2014 (Thursday)
Labour Day
All states
13 May 2014 (Tuesday)
Wesak Day
All states
7 June 2014 (Saturday)
Agongs birthday
All states
15 July 2014 (Tuesday)
Nuzul Al-Quran
Kelantan, Pahang, Perak, Perlis, Penang, Selangor

& Terengganu
28 July 2014 (Monday)
Hari Raya Puasa *
All states
31 August 2014 (Sunday)
Merdeka Day
All states
16 September 2014 (Tuesday)
Malaysia Day
All states
5 October 2014 (Sunday)
Hari Raya Qurban *
All states
6 October 2014( Monday)
Hari Raya Qurban (Day 2)
Kelantan & Terengganu only
23 October 2014 (Thursday)
Deepavali *
All states except WP Labuan & Sarawak
25 October 2014 (Saturday)
Awal Muharram (Maal Hijrah)
All states
11 December 2014 (Thursday)
Sultan Selangors birthday
Selangor
25 December 2014 (Thursday)
Christmas Day
All states
29 July 2014 (Tuesday)
* To be confirmed. Subject to change
2014 School Holidays

21 March - 29 March
Term 1 Holidays
Kedah, Kelantan & Terengganu
22 March - 30 March
Term 1 Holidays
Perlis, Penang, Perak, Selangor, N. Sembilan, Melaka, Johor,

Pahang, Sabah, Sarawak, WP Kuala Lumpur, Putrajaya,
Labuan
27 May - 14 June
Mid-year Holidays
28 May - 15 June
Mid-year Holidays

Labuan
12 September - 20 September
Term 2 Holidays
13 September - 21 September
Term 2 Holidays

Labuan
21 November - 3 January
End-year Holidays
22 November - 4 January
End-year Holidays

Labuan
2014
JANUARY 2014
Sun
T1 lipase protein crystal at different crystallization

temperature: 16C (top), 20C (middle) and 40C.
T1 lipase is the first protein crystal in Malaysia.
Mon
Tue
Wed
Thu
Fri
Sat
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
FEBRUARY 2014
DECEMBER 2013
3D structure of T1 lipase
10
11
12
13
14
15
16
17
18
19
20
21
10
11
12
13
14
15
22
23
24
25
26
27
28
16
17
18
19
20
21
22
29
30
31
23
24
25
26
27
28
Public Holidays (January 2014):

Reference:
Leow, T. C., Rahman, R. N. Z. A., Basri, M. and Salleh, A. B. (2007) High Temperature Crystallization of
Thermostable T1 Lipase. Crystal Growth & Design. 7: 406-410
Hiroyoshi Matsumura, Takahiko Yamamoto, Leow Thean Chor, Abu Bakar Salleh, Mahiran Basri, Tsuyoshi Inoue,
Yasushi Kai, Raja Noor Zaliha Raja Abd Rahman (2008) Novel cation-p interaction revealed by crystal structure
of thermoalkalophilic lipase, PROTEINS: Structure, Function, and Bioinformatics 70 (2): 592-598
1st - New Year

14th - Maulidur Rasul
14th - Yang di-Pertuan Besar Negeri Sembilan's Birthday
17th - Thaipusam
19th - Sultan of Kedah's Birthday
31st - Chinese New Year (1st)
FEBRUARY 2014
Sun
Mon
Tue
Wed Thu
Sat
Fri
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
Top: T1 lipase space crystal (1.35 ). Microgravity

crystallization produces better electron density
map thus an improved protein structure for better
understanding.
Top: T1 lipase ground

crystal (1.7 ). Poor
electron density map.
MARCH 2014
JANUARY 2014
10
11
12
13
14
15
16
17
18
10
11
12
13
14
15
19
20
21
22
23
24
25
16
17
18
19
20
21
22
26
27
28
29
30
31
23
24
25
26
27
28
29
30
31
Public Holidays (February 2014):

1st - Chinese New Year (2nd )
1st - Federal Territory Day
HDPCG (High Density Protein Crystal Growth)

was used for protein crystallization in space
with T1 lipase was used as model protein.
Ground crystal of T1 lipase was compared
with its space crystal.
National Angkasawan Programme:

Soyuz TMA-11 was launched to space on October 10th, 2007
and Soyuz TMA-10 landed on Earth on October 21st, 2007.
MARCH 2014
FEBRUARY 2014
Sun
F
Mon Tue
Wed Thu
Fri
Sat
1
1
2
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
APRIL 2014
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Public Holidays (March 2014):

4th - Anniversary of Installation of Sultan of
Terengganu
Top: One set of JCB-HD (JAXA crystallization apparatus)

contains 12 capillaries with 4 protein samples for 3 capillary
for each experiment.
Collaboration between EMTECH and JAXA (Japanese Aerospace Exploration Agency) to

grow protein crystals in Kibo or JEM (Japanese Experiment Module) that is designed
for microgravity experiment. It is a Japanese science module for the ISS developed by
JAXA. JEM is the first human-rated spaced facility for conducting scientific research
activities on orbit.
Six flights offered in three years from July 2009
until March 2013 with each flight duration between
2-4 months.
V171S
L2 lipase
T1 lipase
L42 lipase
D311E
APRIL 2014
Sun
Mon
Tue
Wed Thu
Fri
Sat
10
11
12
L2 lipase ground crystals of two different shapes. Diamond
shaped (orthorhombic) (left) and rod-shaped (right).
13
14
15
16
17
18
19
20
21
22
23
24
25
26
L2 lipase space crystals. Orthorhombic shaped crystal is

the one in blue (left).
27
28
29
30
MAY 2014
MARCH 2014
10
10
11
12
13
14
15
11
12
13
14
15
16
17
16
17
18
19
20
21
22
18
19
20
21
22
23
24
23
24
25
26
27
28
29
25
26
27
28
29
30
31
30
31
Solved 3D structure of L2 lipase (1.5 )
Public Holidays (April 2014):

15th - Declaration of Malacca as a
Historical City
18th - Good Friday
19th - Sultan of Perak's Birthday
26th - Sultan of Terengganus Birthday
Reference:
Fairolniza Mohd Shariff, Raja Noor Zaliha Raja Abd. Rahman,
Mohd Shukuri Mohd Ali, Adam Thean Chor Leow, Mahiran Basri
and Abu Bakar Salleh (2010) Crystallization And Preliminary X-ray
Crystallographic Analysis Of A Highly Thermostable L2 Lipase From
A Newly Isolated Bacillus sp. L2. Acta Crystallographica Section F,
F66:715-717
MAY 2014
Sun
Ground-grown (top) and

space-grown (right) crystals
of T1 lipase in capillary
using counter diffusion
method.
Superimpose of chains A and B for

ground-grown and space-grown of T1
lipase. (Top) Superimpose of chain A
for both T1 lipase crystal (magenta
for space crystal) with RMSD of
0.2185. (Left) Superimpose of
chain B (gray for space crystal) with
RMSD of 0.4214 .
Mon Tue
Fri
Sat
Wed Thu
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
APRIL 2014
Snapshots of ground-grown crystal

(1.1) and space-grown crystal (1.3
) electron density map countered
at 1 sigma level displayed in COOT.
JUNE 2014
10
10
11
12
13
14
11
12
13
14
15
16
17
15
16
17
18
19
20
21
18
19
20
21
22
23
24
22
23
24
25
26
27
28
25
26
27
28
29
30
31
29
30
Public Holidays (May 2014):

Reference:
Sayangku Nor Ariati Mohamad Aris, Adam Leow Thean Chor, Mohd
Shukuri Mohamad Ali, Mahiran Basri, Abu Bakar Salleh, and Raja Noor
Zaliha Raja Abd. Rahman, Crystallographic Analysis of Ground and
Space Thermostable T1 Lipase Crystal Obtained via Counter Diffusion
Method Approach, BioMed Research International, vol. 2014, Article
ID 904381, 8 pages, 2014. doi:10.1155/2014/904381
1st - Labour Day

7th - Hari Hol Pahang
13th - Wesak Day
17th - Raja Perliss Birthday
27th - Israk & Mikraj
30th & 31st - Pesta Kaamatan Sabah
JUNE 2014
Sun
Mon
Tue
Wed
Thu
Fri
Sat
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
Top: Diffraction patterns of thermostable mutant lipase
D311E. The resolution is ~2.1 at the edge with starting
position; distance: 50.00 mm, 2 theta: 22.00, Omega:
31.64, Phi: 271.48, Chi: 30.01 and ending position;
distance: 50.00 mm, 2 theta: 22.00, Omega: 31.64, Phi:
271.98, Chi: 30.01.
29
Bottom: D311E lipase crystal grown using sitting drop method with 0.1 M MES pH 5.5, 0.1 M Sodium phosphate, 0.1 M
Potassium phosphate, and 1.5 M NaCl as precipitant reagent.
30
MAY 2014
JULY 2014
10
11
12
10
13
14
15
16
17
18
19
11
12
13
14
15
16
17
20
21
22
23
24
25
26
18
19
20
21
22
23
24
27
28
29
30
31
25
26
27
28
29
30
31
Top: Snapshot of ion pair interaction formation of D311E lipase

crystal structure at residue between Glu311 and four residues
(Arg 274, Thr 278, Gly 279 and Arg 303).
Reference:
Public Holidays (June 2014):

1st & 2nd - Hari Gawai Sarawak
7th - Agongs Birthday
29th - Awal Ramadhan
Rudzanna Ruslan, Raja Noor Zaliha Raja Abd. Rahman, Thean Chor
Leow, Mohd Shukuri Mohamad Ali, Mahiran Basri, Abu Bakar Salleh
(2012) Improvement of Thermal Stability via Outer-loop Ion Pair
Interaction of Mutated T1 Lipase, International Journal of
Molecular Sciences 13, 943-960
JULY 2014
Sun
Wed Thu
Fri
Sat
Mon Tue
Optimization of S2F lipase protein

crystal. Optimum precipitant at
0.75 M, and at pH 9.0
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Top: S2F lipase space crystal

S2F is a mutant of thermostable L2, and was mutated at
its N-terminal with the second amino acid, serine being
substituted to phenylalanine.
JUNE 2014
AUGUST 2014
10
11
12
13
14
15
16
17
18
19
20
21
10
11
12
22
23
24
25
26
27
28
17
18
29
30
24
25
Public Holidays (July 2014):

7th - Georgetown Heritage Day
12th - Penangs Governors Birthday
15th - Nuzul Al-Quran
28th & 29th - Hari Raya Puasa
13
14
15
16
19
20
21
22
23
26
27
28
29
30
31
Top: 3D Structure of S2F lipase
AUGUST 2014
Sun
Mon
Tue
Wed
Thu
Fri
Sat
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Ground crystal of ARM lipase produced in the

optimized crystallization formulation that is 0.1
M Tris-HCl pH 8.0 and 12% (v/v) PEG 20000.
Space-grown crystals of ARM lipase obtained in a

gel-tube capillary using 0.1 M MES monohydrate pH
6.5 and 12% (v/v) PEG 20000 as precipitant, with
approximate dimension of 0.05 x 0.01 x 0.20 mm.
31
SEPTEMBER 2014
JULY 2014
10
11
12
10
11
12
13
13
14
15
16
17
18
19
14
15
16
17
18
19
20
20
21
22
23
24
25
26
21
22
23
24
25
26
27
27
28
29
30
31
28
29
30
Public Holidays (August 2014):
Top: 3D structure of ARM lipase (1.15 )
31st - National Independence Day (Merdeka Day)
SEPTEMBER 2014
Sun Mon
Tue Wed
Thu
Fri
Sat
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Enhanced elastase crystal size. Elastase crystal. (Top) Crystal

growth during crystal screening; (middle) crystal growth using
the hanging drop method; and (bottom) crystals in the capillary
using the microseeding counter-diffusion method.
AUGUST 2014
OCTOBER 2014
10
11
12
13
10
11
12
13
14
15
16
14 15
16
17
18
19
20
17
18
19
20
21
22
23
21 22
23
24
25
26
27
24
25
26
27
28
29
30
28 29
30
31
Public Holidays (September 2014):

13th - Sarawak Governor's Birthday
16th - Malaysia Day
Reference:
Mohd Shukuri Mohamad Ali, Zatty Syamimi @ Adura Mat Said, Raja
Noor Zaliha Raja Abd Rahman, Adam Leow Thean Chor, Mahiran
Basri, Abu Bakar Salleh (2013). Capillary-Seeding Crystallization and
Preliminary Crystallographic Analysis of a Solvent-Tolerant Elastase
from Pseudomonas aeruginosa Strain K. International Journal of
Molecular Sciences, 14 (9), 17608-17617
OCTOBER 2014
Sun
Mon
Tue
Wed
Thu
Fri
Sat
10
SEPTEMBER 2014
11
M
1
T
2
W
3
T
4
F
5
S
6
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
NOVEMBER 2014
12
19
13
20
14
21
15
22
16
23
17
24
S
1
18
25
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Public Holidays (October 2014):
26
27
28
29
30
4th - Sabah Governors Birthday

5th - Hari Raya Haji
10th - Malacca Governor's Birthday
23rd - Deepavali (Diwali)
24th - Sultan of Pahangs Birthday
25th - Awal Muharram (Maal Hijrah)
31
Left: L42 crystal in counter-diffusion method. Right: Diffraction pattern

of L42 crystal in counter-diffusion (~2.0 ).
Top: Solved 3D structure of L42 Lipase (1.4 )
Reference:
Left: L42 crystal in vapor-diffusion. Right: Diffraction pattern of L42

crystal in vapor-diffusion(~2.4 ).
Mohd Saif Khusaini, Raja Noor Zaliha Raja Abd Rahman, Mohd Shukuri Mohd Ali, Adam
Leow Thean Chor(2011). Preliminary X-ray Crystallographic Analysis of Thermostable
Lipase 42. Acta Crystallographica section F 67, 401403
NOVEMBER 2014
Sun
Mon
Tue
Wed
Thu
Fri
Sat
1
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Crystals of LipPI12, a cold active bifunctional lipase

with protease activity from Antarctic microorganism
Pi 12 grown in 0.1M HEPES sodium buffer at pH 7.5
with precipitants 20% w/v of PEG 4000 and 10% v/v
of 2-propanol. The crystals take form in a shape of
needle cluster.
OCTOBER 2014
DECEMBER 2014
10
11
12
13
10
11
12
13
14
15
16
17
18
19
20
14
15
16
17
18
19
20
21
22
23
24
25
26
27
21
22
23
24
25
26
27
28
29
30
28
29
30
31
Top: 3D structure of PI 12
Public Holidays (November 2014):

11th & 12th - Sultan of Kelantan's Birthday
22nd - Sultan of Johors Birthday
29th - Hari Hol Almarhum Sultan Iskandar Johor
DECEMBER 2014
Sun
Mon
Tue
Wed
Thu
Fri
Sat
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
NOVEMBER 2014
JANUARI 2015
10
10
11
12
13
14
15
11
12
13
14
15
16
17
16
17
18
19
20
21
22
18
19
20
21
22
23
24
23
24
25
26
27
28
29
25
26
27
28
29
30
31
30
Public Holidays (December 2014):

11th - Sultan Selangors Birthday
25th - Christmas
From left to right, from up to down:

The EMTECHs protein crystals: F16L/S157g lipase
(ground), F16L/S157g lipase (space), F16L lipase
(ground), F16L lipase (space), L2 lipase (ground), L2
lipase (space), L42 lipase (ground), PI12 lipase, S2F
lipase (ground), T1 lipase (ground), T1 lipase
(space), V171S, D311E lipase, ARM lipase (ground),
HZ lipase (space), HZ lipase (ground).

WWW - Emtech.upm - Edu.my Document EMTech Calendar

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

WWW - Emtech.upm - Edu.my Document EMTech Calendar

Uploaded by

Copyright:

Available Formats

Celebrating the International Year of

Enzyme and Microbial Technology Research Centre

Enzyme and Microbial Technology Research

UPM Academic Calendar

9 September 2013 - 3 November 2013

4 November - 10 November 2013

11 November 2013 - 22 December 2013

23 December 2013 - 29 December 2013

30 December 2013 - 12 January 2014

- Breaks Between Semester

13 January 2014 - 16 February 2014

17 February 2014 - 6 April 2014

7 April 2014 - 13 April 2014

14 April 2014 - 1 June 2014

2 June 2014 - 8 June 2014

9 June 2014 - 22 June 2014

*** Final Semester Break

13 January 2014 - 16 February 2014

23 June 2014 - 10 August 2014

11 August 2014 - 18 August 2014

## Chinese New Year

31 January - 1February 2014

Friday & Saturday

*** Hari Raya Puasa

Monday & Tuesday

THIRD SEMESTER (SPECIAL)

EMTECH Activity Calendar

EMTECH Colloquium 2014

Workshop on Protein Crystallography

From left to right: Freeze dryer, spray dryer, bioreactor (7.5 L)

2014 Public Holidays

New Year Celebration

All states except Johor, Kedah, Kelantan, Perlis &

14 January 2014 (Tuesday)

Prophet Muhammads Birthday

17 January 2014 (Friday)

Kuala Lumpur, Putrajaya, Johor, N. Sembilan,

31 January 2014 (Friday)

Chinese New Year (Day 1)

All states except Kelantan & Terengganu

1 February 2014 (Saturday)

Chinese New Year (Day 2)

1 May 2014 (Thursday)

13 May 2014 (Tuesday)

7 June 2014 (Saturday)

15 July 2014 (Tuesday)

Kelantan, Pahang, Perak, Perlis, Penang, Selangor

28 July 2014 (Monday)

Hari Raya Puasa *

31 August 2014 (Sunday)

16 September 2014 (Tuesday)

5 October 2014 (Sunday)

Hari Raya Qurban *

6 October 2014( Monday)

Hari Raya Qurban (Day 2)

Kelantan & Terengganu only

23 October 2014 (Thursday)

All states except WP Labuan & Sarawak

25 October 2014 (Saturday)

Awal Muharram (Maal Hijrah)

11 December 2014 (Thursday)

Sultan Selangors birthday

25 December 2014 (Thursday)