Professional Documents
Culture Documents
Spring 2015
(use this sheet to guide your reading in the lab manual)
Lab 1, Week of : January 19th.
Part 1: Introduction to the lab, administration and safety procedures.
1. Review of student guidelines and lecture on safety procedures using CST Student Guidelines manual.
You must have the Safety manual with you to sign a safety training sheet to be in the labs.
Part 2: Introduction to the Microscope; Lab 1 in Symbiosis lab manual
Review the objectives on page 1 on the lab chapter. You should be able to:
1. Identify and be knowledgeable about usage for the compound and stereomicroscopes.
2. Distinguish between prokaryotic and eukaryotic cells
3. Distinguish between the major structures of plant and animal cells
4. Recognize in cells the increasing complexity of organization and understand the evolutionary significance.
1. The 1st, 2nd & 3rd exercises introduce the student to the use of, and care for, our compound and stereo
microscopes. The goal of this part is to learn how to use the microscopes properly for good results and to avoid
breakage problems
Prepared slides used for this part are:
a.) Letter e; used to demonstrate basic scope techniques
b.) Colored threads; used to demonstrate more advanced aspects of the microscope.
2. The 4th exercise on the electron microscope is omitted.
3. The 5th exercise starts the investigation of cell structure and organization.
This exercise follows the same basic procedures learned in part 1, but has different specimens for
observations, both prepared slides and live material. This part introduces the student to the material for the next
series of labs, Bacteria, Protists and Plants. What students learn here with the prepared and live material will
help them in the following labs, particularly the protist lab covering singled cell organism.
a. Lab study A; Unicellular Organisms.
1. mixed bacterial species (bacilli, cocci, spirilla)
2. cyanobacteria example. slide of mixed cyanobacteria.
3. prepared slides of amoeba and paramecia, or mixed protists slide
b. Lab study B; Aggregate and Colonial organisms.
Prepared slides, some live material.
c. Lab study C; Multicellular organisms
Students will make wet mounts here of animal and plant tissues.
d. Lab Study D; Unknown culture.
Prepared slides and pond water used for samples.