MCQ Bio-Chemistry.

Prof, Bun Seangkhin

1. Which of the following sugars is absorbed by facilitated transport?

a. Galactose
b. Fructose
c. Xylose
d. Glucose
e. Arabinose
2. Amylase present in saliva is:
a. -amylase
b. -amylase
c. -amylase
d. All of the above
e. None of the above
3. The absorption of glucose is decreased by the deficiency of:
a. Vitamin A
b. Vitamin D
c. Thiamine
d. Vitamin B12
e. Folic acid
4. For activity of salivary amylase. Which of the following is required as activator:
a. HCO3 ion
b. Presence of Na+
c. Presence of K+
d. Chloride ion
e. None of the above
5. Which of the following hormone increases the absorption of glucose
from GI tract?
a. Insulin
b. Thyroid hormones
c. Glucagon
d. FSH
e. LH
6. UDPGlucose is converted to UDPglucuronic acid by:
a. ATP
b. GTP
c. NADP+
d. NAD+
e. FAD
7. All of the following compounds are intermediates of TCA cycle except:
a. Malate
b. Pyruvate
c. Oxaloacetate
d. Fumarate
e. Succinate
8. In conversion of lactic acid to glucose, three reactions of glycolytic pathway
are circumvented, which of the following enzymes do not participate?
a. Pyruvate carboxylase
b. Phosphoenolpyruvate carboxykinase
c. Pyruvate kinase
d. Glucose-6-phosphatase
e. Fructose-1, 6-biphosphatase
9. MacArdles disease involves a deficiency of the which enzyme?
a. Acid maltase
b. Glucose-6-phosphatase
c. Hepatic phosphorylase
d. Muscle phosphorylase
e. Branching enzyme
10. In the normal resting state of humans, most of the blood
glucose burnt as fuel is consumed by:
a. Liver
b. Brain
c. Kidneys
d. Adipose tissue
e. Muscles
11. A regulator of the enzyme glycogen synthase is:
a. Citric acid
b. 2,3-biphosphoglycerate (2,3-BPG)
c. Pyruvate
d. GTP
e. Glucose-6-PO4

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12. Which of the following compound is a positive allosteric

modifier of the enzyme pyruvate carboxylase?
a. Biotin
b. Acetyl-CoA
c. Oxaloacetate
d. ATP
e. Fructose-6-P
13. A specific inhibitor for succinate dehydrogenase is:
a. Arsenite
b. Malonate
c. Citrate
d. Cyanide
e. Fluoride
14. Most of metabolic pathways are either anabolic (synthetic) or catabolic
(degradation). Which of the following pathways is considered as amphibolic in
nature?
a. Glycogenesis
b. Glycolytic pathway
c. Lipolysis
d. Citric acid cycle
e. Glycogenolysis
15. Transketolase activity is affected in:
a. Biotin deficiency
b. Pyridoxine deficiency
c. PABA deficiency
d. Thiamine deficiency e. Manganese deficiency
16. The following metabolic abnormalities occur in Diabetes mellitus, except:
a. Increase plasma FFA
b. Increased pyruvate carboxylase activity
c. Decrease lipogenesis
d. Decrease gluconeogenesis
e. Increase PEPcarboxykinase activity
17. A substance that is not an intermediate in the formation of D-glucuronic acid from
glucose is:
a. Glucose-1-P
b. 6-phosphogluconate
c. Glucose-6-P
d. UDPGlucose
e. UDPglucuronate
18. Von Gierkes disease is characterised by a deficiency of which enzyme?
a. Glucokinase
b. Glucose-6-phosphatase
c. -1, 6-glucosidase
d. Glycogen synthase
e. De-branching enzyme
19. The hydrolysis of glucose-6-P is catalysed by a phosphatase that is not found
in which of the following?
a. Liver
b. Kidney
c. Muscle
d. Small intestine
e. None of the above
20. An essential for converting glucose to glycogen in Liver is
a. Lactic acid
b. GTP
c. CTP
d. UTP
e. Pyruvic acid
21. Which of the following is a substrate for aldolase activity in glycolytic pathway?
a. Glyceraldehyde-3-P
b. Glucose-6-P
c. Fructose-6-P
d. 1,3-diphosphoglycerate
e. Fructose-1,6-bi-P
22. Which of the following hormones is not involved in carbohydrate metabolism:
a. Cortisol
b. ACTH
c. Glucagon
d. Vasopressin
e. Growth hormone

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23. Dehydrogenases involved in HMP shunt are specific for:

a. NADP+
b. NAD+
c. FAD
d. FMN
e. None of the above
24. Which of the following enzymes in glycolytic pathway is
inhibited by fluoride?
a. Glyceraldehyde 3-P-dehydrogenase
b. Phosphoglycreate kinase
c. Pyruvate kinase
d. Enolase
e. Aldolase
25. Micelles formed in the intestine have which of the following properties?
`
a. They are absorbed intact in the duodenun
b. They contain mostly triacylglycerol (TG)
c. They facilitate transfer of hydrolytic products of TG to intestinal mucosal cell
d. They are secreted by cells lining the intestine
e. They are mainly absorbed from stomach wall
26. Dietary TGs (triacylglycerols) are absorbed from the intestinal lumen after
hydrolysis mainly as:
a. Fatty acids and glycerol
b. Fatty acids and two monoacylglycerol
c. Fatty acids and , -diglyceride
d. Acyl-CoA and glycerol
e. Glycerol-phosphate and fatty acids
27. The gastric lipase is inactive because of the following reasons except:
a. No emulsifications of fats take place in stomach
b. The enzyme is secreted in small quantity
c. pH of gastric juice is not conducive for its action
d. The gastric juice is highly acidic
e. The conc. of bile salts is high in gastric juice
28. Delay in the rate of gastric emptying is due to the action of:
a. Pancreozymin
b. Enterokinase
c. Enterogastrone
d. CCK
e. Gastric lipase
29. Ca++ facilitates action of lipase:
a. By emulsification
b. By inhibiting emulsification
c. By soap formation
d. By formation of micelle
e. By formation of tiny droplets
30. A pathway that requires NADPH as a cofactor is:
a. Fatty acid oxidation
b. Extramitochondrial de novo fatty acid synthesis
c. Ketone bodies formation
d. Glycogenesis
e. Gluconeogenesis
31. The committed step in the biosynthesis of cholesterol from acetyl-CoA is:
a. Formation of acetoacetyl-CoA from acetyl-CoA
b. Formation of mevalonate from HMG-CoA
c. Formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA
d. Formation of squalene by squalene synthetase
e. Formation of lanosterol from squalene by cyclisation.

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32. The most important source of reducing equivalents for FA synthesis in the Liver is:
a. Glycolysis
b. HMP-shunt
c. TCA cycle
d. Uronic acid pathway
e. Gluconeogenesis
33. All of the following tissues are capable of using ketone bodies, except:
a. Brain
b. Renal cortex
c. Red blood cells
d. Cardiac muscle
e. Skeletal muscle
34. Ketone bodies are synthesised from fatty acid oxidation products by which of the
following organs?
a. Skeletal muscles
b. Kidney
c. Erythrocytes
d. Brain
e. Liver
35. Which of the following cofactors of their derivatives must be present for the
conversion of acetyl-CoA to Malonyl-CoA in extramitochondrial FA synthesis?
a. FAD
b. FMN
c. ACP
d. NAD+
e. Biotin
36. All statements regarding 3-OH-3 methly glutaryl CoA are true, except:
a. It is formed in the cytoplasm
b. Required in ketogenesis
c. Involved in synthesis of FA
d. An intermediate in cholesterol biosynthesis
e. Enzyme involved in HMG-CoA synthase
37. Which of the following lipoproteins would contribute to a measurement of plasma
cholesterol in a normal individual following a 12 hour fast?
a. Chylomicrons
b. VLDL
c. Both VLDL and LDL
d. LDL
e. Both chylomicrons and LDL
38. All statements regarding ketone bodies are true except:
a. They may result from starvation
b. They are formed in kidneys
c. They include acetoacetic acid and acetone
d. They may be excreted in urine
e. They are present in high concentration in uncontrolled Diabetes mellitus
39. Brown adipose tissue is characterised by the following:
a. Present in large quantities in adult humans
b. Mitochondrial content higher than white adipose tissue
c. Oxidation and phosphorylation are tightly coupled
d. Absent in hibernating animals
e. All of the above
40. Ketosis is partly ascribed to:
a. Over production of glucose
b. Under production of glucose
c. Increased carbohydrate utilization
d. Increased fat utilisation
e. Increased pyruvate in liver
41. Triacyl glycerol (TG) Present in VLDL is hydrolysed by:
a. Intestinal lipase
b. Lipoprotein lipase
c. Hormone sensitive TG lipase
d. Pancreatic lipase
e. Co-lipase
42. Carnitine is synthesised from:
a. Lysine
b. Serine
c. Choline
d. Arginine
e. Threonine
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43. A metabolite which is common to pathways of cholesterol biosynthesis from acetylCoA and cholecaliferol formation from cholesterol is:
a. Zymosterol
b. Lumisterol
c. Ergosterol
d. 7-Dehydrocholesterol
e. Pregnenolone
44. Acetyl-CoA required for extramitochondrial FA synthesis is produced by:
a. Pyruvate dehydrogenase complete b. Thiolase
c. Acetyl-CoA synthase
d. Carnitine-acyl tranferase
e. Citrate Lyase
45. Biosynthesis of TG and lecithin both require an intermediate
a. Monoacyl glycero-(P)
b. Phosphatidic acid
c. Phosphatidyl ethanolamine d. Phosphatidly cytidylate
e. Phosphatidyl adenylate
46. The rate limiting step in cholesterol biosynthesis is:
a. Squalene synthetase
b. Mevalonate kinase
c. HMG-CoA synthetase
d. HMG-CoA reductase
e. Thiolase
47. Pancreatic juice contains all of the following, except:
a. Trypsinogen
b. Lipase
c. Cholecystokinin
d. Chymotrypsinogen
e. Amylase
48. The milk protein in the stomach in an adult is digested by:
a. Pepsin
b. Rennin
c. HCI
d. Trypsin
e. Chymotrypsinogen
49. Carboxypeptidase A enzyme of pancreatic juice contains:
a. Copper
b. Zinc
c. Magnesium
d. Manganese
e. Cobalt
50. The enzyme collagenase of pancreatic juice hydrolyses collagen present in:
a. Milk
b. Eggs
c. Cereals
d. Meat
e. Soyabeans
51. The zymogen form trypsinogen of pancreatic juice is converted to active trypsin
by:
a. Pepsin
b. Enterocrinin
c. Enterokinase
d. Rennin
e. Gastrin
52. Which of the following regarding digestion of proteins are correct, except:
a. Pepsinogen is activated by autoactivation (pH-2) or autocatalysis
b. The major products or protein hydrolysis are large petides and some free amino acids
c. Most of the digested proteins are absorbed in the intestine in the form of polypeptides
d. Trypsin and chymotrypsin are secreted by the pancreas as inactive zymogens
e. Enterokinase activates pancreatic trypsinogen
53. Rennin acts on casein of milk in infants in presence of:
a. Mg++
b. Zn++
c. Co++
d. Ca++
e. Mn++

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54. During intestinal proteolysis:

a. Enterokinase converts trypsinogen to trypsin
b. Involves conformational change, but no change in covalent structure
c. Trypsin activates procarboxypeptidase A and proelastase
d. a + b true
e. a + c true
55. Pepsin contains large amounts of which of the following amino acids:
a. Aromatic amino acids
b. Acidic amino acids
c. Basic amino acids
d. Sulphur containing amino acids
e. Neutral amino acids
56. D-amino acids are absorbed by:
a. Active transport
b. In presence of a carrier molecule
c. In presence of sodium ions d. Passive diffusion
e. None of the above
57. Chymotrypsin hydrolyses the peptide linkages containing
a. Phenyl alanine and tyrosine b. Phenyl alanine and lysine
c. Tyrosine and lysine
d. Tyrosine and leucine e. Phenylalanine and leucine
58. Quantitatively the most important enzyme involved in formation of NH3 from
amino acids in humans is:
a. L-amino acid oxidase
b. Serine dehydratase
c. Glutamate dehydrogenase
d. Histidase
e. Desulfhydrase
59. The two nitrogen atoms in urea arise from:
a. Ammonia and glutamine
b. Glutamine and aspartic acid
c. Glutamine and glutamic acid
d. Ammonia and aspartic acid
e. Glutamine and alanine
60. A product of the series of reactions that converts carbamoyl-(P) to urea is:
a. Arginine
b. Citrulline
c. Fumarate
d. Aspartate
e. Adenosine triphosphate
61. Ornithine has all of the following properties except:
a. It is an intermediate of urea cycle
b. It is not found in proteins
c. It is glucogenic
d. It can be formed from arginine
e. It is a mono-amino mono-carboxylic acid
62. Epinephrine is formed from norepinephrine by:
a. Hydroxylation
b. Oxidative deamination
c. Decarboxylation
d. N-methylation
e. O-methylation
63. The rate limiting step in the biosynthetic pathway of catecholamines is:
a. The hydroxylation of phenylalanine
b. The hydroxylation of tyrosine
c. The reduction of biopterine
d. The formation of dopamine
e. None of the above
64. Which of the following compounds serve as a primary link between the TCA cycle
and the urea cycle?
a. Malate
b. Citrate
c. Oxaloacetate
d. Fumarate
e. Succinate
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65. Serine can be a percussor of each of the following, except:

a. Cysteine
b. Methionine
c. Ethanolamine
d. Glycine
e. Choline
66. The rate of excretion of certain substances in 24-hr sample of urine can be used to
estimate the rate of muscle protein loss. Which of the following substances falls into
this category?
a. Ammonia
b. Urea
c. Uric acid
d. 3-Methyl histidine
e. Ergothioneine
67. Tryptophan is best described by which of the following statement?
a. It produces thyroid hormones
b. Is a precursor for melanin
c. It is a precursor of the pineal hormone melatonin
d. It produces catecholamines
e. It is glucogenic only
68. Which of the following amino acids are required for synthesis of creatine?
a. Arginine, Asp, 'active' methionine
b. Arginine, glycine 'active' methionine
c. Arginine, lysine, methionine
d. Glycine, Asp, ornithine
e. Arginine, glutamic acid, 'active' methionine
69. The following statements regarding creatinine are correct except:
a. Blood levels of creatinine is a good indicator of kidney function
b. Although 24-hr excretion rate is very constant, there is a marked diurnal variation
c. Urinary creatinine levels give a measure of muscle mass
d. Excretion of creatinine is urine of adults is very constant from day-to-day
e. Cratinine is an anhydride of creatine-(P)
70. Which of the following amino acids on degradation produces a glucogenic
intermediate of TCA cycle and ketone body?
a. Glycin
b. Phenylalanine
c. Alanine
d. Cysteine
e. Serine
71. Reactions of urea cycle take place in liver cells
a. In cytosol
b. Only in lysosomes
c. In mitochondrial matrix
d. Both cytosol and mitochondrial matrix.
e. Both cytosol and lysosomes
72. Breakdown of amino acid tryptophan begins with formation of:
a. Urocanic acid
b. Nicotinic acid
c. Anthranilic acid
d. N-formyl kynurenine
e. None of the above
73. Urinary oxalates in small quantities may arise from:
a. Tyrosine
b. Glycine
c. Serine
d. Citrates
e. Oxaloacetate
74. A high ATP/AMP ratio will inhibit which of the following enzyme?
a. Glyceraldehyde-3-P dehydrogenase
b. Pyruvate kinase
c. Enolase
d. Phosphofructokinase
e. Glucose-6-P dehydrogenase

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Reference
https://books.google.com.kh/books?id=I3gC0bX_IKAC&pg=PA323&lpg=PA323&dq#v =
onepage&q&f=false
Answers researched by Hul Vichhai
Textbook of Medical Biochemistry,

1-2 page
6-24 page
30-31 page
39-46 page
58-69 page
74
page

323
389-390
460
462
529
534

By M. N. Chatterjea, Rana Shinde

3-5
25-29
32-38
47-57
70-73

page
page
page
page
page

324
397
461
469
530

God Bless you abundantly.

Dieu te bnisse abondamment.

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