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Introduction:
Aseptic technique is a crucial experimental condition in a
microbiology lab. Preventing the contamination of a sample with
foreign microbes from the environment is the main focus of the present
experiment. The participants were required to maintain a sanitary
environment isolating the samples to be contaminated with microbes
that were not intentionally introduced into the growth medium.
Secondly, but equally important, was to prevent the contamination,
with possible pathogenic bacteria, of the lab members while handling
the cultures.
The experiment introduced a challenging condition to the sterile
technique. The experimental procedures were performed on benchtop.
The myriad of microbes floating in the atmosphere and on the
benchtop, can constantly contaminate the samples, therefore careful,
fast and precise handling was necessary to achieve success in the
experiment.
It
can
be
hypothesized
that
applying
successful
aseptic
plates was only partially opened when the growth medium was
dispensed, to ensure minimal exposure to atmosphere. The plates were
placed, treated and handled in various conditions. The lid from Plate C
was removed and the growth medium was exposed to the atmosphere
for 10 minutes. Plate D was kept closed. The growth medium from
Plate E was placed the fume hood for 10 minutes and the plate was
exposed to the air inside the hood. Plate F was kept closed. The lid
from Plate G was partially opened to minimize exposure to the
environmental air and the growth medium was touched with the finger
that was dirty (finger touched hair, saliva and the surface of the sink).
Plate H lid was partially removed to prevent air from atmosphere to
contaminate the sample and growth medium was touched with a clean
finger (the finger was cleaned with 70% ethanol). The lid from Plate I
was partially opened to ensure minimal exposure to the environment
air and 50 L of E. coli culture was dispensed in the growth medium.
3
The lid from plate J was partially opened to ensure minimal exposure to
atmosphere and 50 L of L-Broth was dispensed in the growth
medium. The plates were later stored in an incubator at 37 and they
incubated for 48 hours. Along with the plates the bottle containing Lbroth that was used was stored in the incubator for 48 hours to ensure
that growth medium was not contaminated with any microbes. After 48
hours the results were documented and the data recorded was used to
determine the validity of the hypothesis.Results:
be
predicted
that,
for
future
experiments,
the
level
of
careful handling of the samples, checking the direction and the amount
of airflow inside the hood.
Conclusion
Analyzing the recorded data, it can be concluded that the
experiment was successful and the aseptic technique used was
properly and thoroughly applied. The present experiment proves that
sterile technique can be achieved even in more challenging condition
when the procedures are completed on an open surfaces such as
bench top. Further studies can be address more challenging conditions
for aseptic techniques than the settings in the present experiment.
These studies can provide valuable information and procedures that
can be used when a sterile technique is required in harsh conditions
outside a microbiology lab (surgeries in the field, first aid in remote
places, making water drinkable, etc.)