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Background study
Problem Statement
Significant of study
The causal pathogen in grouper can be detected
and the prevention or treatment can be applied
in the future.
This study will provide awareness about
diseases in grouper.
By using scanning electron microscope, the
causal pathogen can be identified quickly.
Literature Review
: Animalia
Phylum
: Chordata
Class
: Actinopterygii
Order
: Serranidae
Family
: Serranidae
Genus
: Epinephelus
Species
:Epinephelus fuscoguttatus
Species
Pathogen
Cromileptes altiveli,
Protozoan
Epinephelus bontoides, E. coioides, E. malabaricus,
E. tauvina, E. bleekeri, E. malabaricus, E. suillus, E.
tauvina.
References
Nagasawa et al.,
2004
Nagasawa et al.,
2004
Nagasawa et al.,
2004
Nagasawa et al.,
2004
Copepod
Nagasawa et al.,
2004
Isopods
Epinephelus bleekeri,
E. coioides, E. fuscoguttatus, E. lanceolatus, E.
malabaricus and Cromileptes altivelis
Leeches
Nagasawa et al.,
2004
Nagasawa et al.,
2004
The organs that were used in this study are gills, liver
and heart.
Histology Method
Sample fixation
in Davidsons
fixative.
Staining
Mounting
Dehydration
Sample
embedding
Sample slicing
Observation
Sample Preparation
SEM Method
Fixation
Rinse
Postfixation
Rinse
Sputter coater
Dehydration
CPD
Mounting
Samples scanning
Sputter Coater
Scanning
Results
20 samples were used in this study.
Most of the samples showed the clinical
sign such as darkened body color,
excessive mucous secretion, skin ulcers
and pale gills.
Sample
Clinical sign
Sample
Clinical sign
Skin ulcers
Pale gills
Histology
Ten samples were processed.
No pathological changes were found in
the liver and heart samples.
10 out of 20 gills showed pathological
changes such as hyperplasia, gills diffusion
and gill necrosis.
Specimen
Description
Organ: Gills
Observation: No pathological
changes
Total magnification: 100X
Specimen
Description
Organ: Gills
Specimen
Description
Organ: Gills
Specimen
Descrition
Organ: Gills
Suspected organism:
Diplectanum sp.
Total magnification: 100X
Specimen
Description
Organ: Gills
Suspected organism:
Pseudorhabdosynochus spp
Total magnification: 100X
Specimen
Description
Organ: Heart
Observation: No pathological
changes.
Total magnification: 4x
Specimen
Description
Organ: Liver
Observation: No pathological
changes.
Specimen
Description
Organ: Gill
Observation: Trichodina sp.
attached to gills filament.
The Trichodina sp. size is about
30 m.
Specimen
Description
Organ: Gill
Observation: Trichodina sp.
attached to gill arch.
Discussion
Trichodina sp.
Diplectanum sp.
Pseudorhabdosynochus spp.
Conclusion
The causal pathogen that cause mortality in
grouper were found.
Prevention and treatment can be made to
reduce the mortality rate in the farm.
Further Research
References
Abdel-Baki, A.S., Sakran, T., Fayed, H. and Zayed, E. (2011). Trichidona fahaka in
Tertradon fahaka from Nile River, Egypt. Scientific Research and Essays
6:153-1587
FAO, 2012
Leong, T.S. (1997) Control of parasites in cultured marine n-shes in Southeast Asia
an overview. International Journal forParasitology, 27, 1,1771,184
Lio-Po, G., and de la Pena, L. (2004).Diseases of cultured groupers. Retrieved
http://rfdp.seafdec.org.ph/publication/manual/grouper/
Mendez-Vilaz, A., and Diaz, J. (2007). Modern Research and Educational Topics in
Microscopy. Formatex. p122-131
Nagasawa, K. and E. R. Cruz-Lacierda (eds.) (2004). Diseases of cultured groupers.
Southeast Asian Fisheries Development Center, Aquaculture Department, Iloilo,
Philippines. 81 p.
Purivirojkul, W. (2012). Histological Change of Aquatic by Parasitic Infection. Retrieved
from http://www.intechopen.com/books/histopathology-reviews-and-recentadvances/
Pierre, S., Gaillard, S., Prvot-D'Alvise, N., Aubert, J., Leung-Tack, D., Grillasca, J-P. and RostaingCapaillon, O. (2008) Grouper aquculture: Asian Success and Mediterranean Trials.
Aquatic conservation;18: 297-308
Smith, S. and Schwarz, M. S. (2009). Dealing with Trichidina sp. and Trichodina-like
species. Virginia Cooperative Extension. Publication 600-305
Vale, F. F., and Correia, A. C. (2010). Applications of electron microscopy to virus
detection and identification. Microscopy: Science, technology,
applications and educations
Thank You