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Qriz Subnissims - Emyrre Lab quiz - BIOLOGY 2BG|: CELL BIOLOGY FALL 2013 - McMaster Uniwrsity

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- Enzyme Lab quiz

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ALEXANDRA MACDONALD (username: macdonat)

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Written: Sep 29, 2013 11:48 AM - Sep 29,2OL312:18

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Question

O.O1

O.O1 Points

Which enzyme did you conduct the "experiments" with?

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alpha-amylase

beta-amylase

E*

catalase

E*

lipase

E+

maltase

Question 2

O.5

O.5 points

O.5 points

The substrate for this enzyme is...

E*

Triglycerides

Maltose

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r*

i!rl, Starch
Hydrogen peroxide
Protein

Question 3
What was the product of the reaction?

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Quiz Subnissions

- Enzyne

l-ab quiz

- BIOLOGY 2803: CELL BIOLOGY FALL 2013 - McMaster Unircrsity

glucose

Dextrins

water

fatty acids
peptides

4)

maltose

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Question4

O/lpoint

What did you determine the optimum pH for the reaction to be?
7.OO

The correct answer is not displayed for Long Answer type questions.

Question5

t/lpoint

What did you determine the optimal temperature for the reaction to be?
40.00 deg

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Question6

O/lpoint

What was the Vmax for the reaction and what did you determine the Km to be?

Vmax =49.40
Krn =24.7

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QuestionT

O.S/lpoint

What happened to the reaction and the Km value when you added the competitive inhibitor?

the Km increased

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QuestionS

O/lpoint

What happened to the reaction and the Km when you added the non-competitive inhibitor?
The Km increased

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Question9

2/2points

Explain what is meant by a competitive inhibitor and why it gave the result that it did.

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Quiz Suhrissions - Erzyne Labquiz - BIOLOGY2803: CELL BIOLOGY FALL2013- McMaster Uniwrsity

A competetive inhibitor bind to the active site where the substrate is supposed to bind. As such, it becomes
competition between whether the substrate or the cometetive inhibitor will bind to the active site. The
competetive inhibitor slowed the reaction down, it took more time to get 100o/o product, and 07o substrate.

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Question

1O

2 | 2po-nts

Explain what is meant by a non-competitive inhibitor and why it gave the result that it did?
A non-competetive inhibitor is a molecule that binds to the enzyme somewhere other than the active site,
which causes a slight conformational change, making the active site less efficient for binding to the substrate.
When a little bit of the non competetive enzyme was added, the reaction took longer to proceed, when a lot
(90 units) were added, the reaction did not proceed,

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Attempt Score: 6.01 / 10.01

Overall Grade (highest attempt): 6.01 / 10.01

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