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INSTR:L.JOHNSON
March1,2013
NAME:_________________________________
(last)
(first)
I.D. #__________________________________
TA___________________________________
TestFormB
INSTRUCTIONS
Thisexamis10pageslong.Beforestartingmakesureyouhaveallthepages.
Usinganumber2pencil,onthefrontofyourscantronfillintheInstructor(Johnson),Class
(LS3),Hour(12:00or1:00),
WriteandBubbleinyourUID,yourlastnameandfirstname.
OnthebackofyourscantronWriteandBubbleinyourUIDandTestForm(seeabove).
d. Lac repressor
e. Sigma factor
11.
Which of the following factors play a role in two stages of
translation (initiation, elongation, termination)?
a. EF-Tu
b. EF-G elongation, termination (translocates)
c. IF3 initiation, termination (dissociates the ribosome
subunits)
d. a and b
e. b and c
12.
Pol II transcripts are modified at the 5 end with
a. ATP in a 5-3 linkage
b. ATP in a 5-5 linkage
c. GTP in a 5-3 linkage
d. GTP in a 5-5 linkage
13.
Charging of tRNAs by amino acyl synthetases is a two step
process. The first step can be described as:
a. Transesterification
b. Phospho-transfer reaction
c. Adenylylation
d. Hydrolysis
14.
In the second step in the tRNA charging reaction, the
nucleophile is
a. The 2OH on ATP
b. The carboxyl oxygen on the amino acid
c. The 3 OH on the tRNA everything is always 35
d. The nitrogen on the amino acid
15.
16.
a.
b.
c.
a.
b.
c.
d.
17.
is
e. b and c
The expression of eve in stripe 2 of the Drosophila embryo
a.
b.
c.
d.
d. a and c
e. b and c
23.
The Torpedo Model refers to
a. how DNA is synthesized at the replication fork.
b. how RNA is terminated in eukaryotes polymerase keeps
transcribing after cleavage, the 3 end is
exposed/uncapped, RNase jumps up and starts chewing the
RNA
c. how RNA is terminated in prokaryotes
d. how translation is terminated
24.
Which of the following is NOT true about yeast mating type
control?
a. Mcm1 in combination with 1 activates specific genes
true
b. Haploid specific genes are on unless repressed in a diploid
cell true
c. Mcm1 in combination with a1 activates a specific genes
MCM1 will express a1 by itself
d. a1 and 2 combine to form a repressor true; they repress
alpha 1
25.
In the graph above, the peaks of radioactivity look different
from the peaks of OD 260 because
a. the radioactivity is only measuring mRNA and the OD260 is
measuring rRNA.
b. the radioactivity is only measuring newly synthesized RNA
and the OD260 is measuring total RNA, which is mostly
rRNA.
c. the radioactivity is measuring RNA and the OD260 is
measuring protein.
d. All of the above.
26.
Which of the following factors recognizes the TATA box?
a. TFIIA
b. TFIIB
c. TFIID contains TBP as one of its proteins
6
27.
28.
29.
d. THIIE
e. TFIIH
The Gal80p repressor inhibits transcription by
a. binding to Gal3p and blocking activation
b. binding to Gal4p and blocking activation gal80 is really
an anti-activator
c. binding to the GAL1 operator and blocking RNA polymerase
d. binding to the UAS and blocking Gal4p binding
General transcription factors in eukaryotes
a. bind to enhancers to regulate transcription in a tissue
specific manner no, general transcription factors bind to
promoter
b. are responsible for recognizing the TATA box yes
c. are needed at all promoters during transcription initiation
yes
d. b and c
e. all of the above
Alternative splicing
a. allows exons to be spliced together in reverse order.
b. allows exons to be spliced together in the DNA to create
new genes.
c. allows exons to be spliced together in more than one way
to encode more than one protein from a single gene.
d. All of the above.
30.
The first transesterification reaction that takes place during
splicing
a. is catalyzed by snRNPs yes
b. involves the branch point 2OH attacking the 3 splice site
no; A 3 OH attacks 5 splice site!!!
c. occurs after activation of the snRNP by adenylylation
d. a and b
e. all of the above
31.
Which of the following is NOT true of splicing?
a. U2 snRNP binds to the branch point true
b. U1 snRNP binds to the 3 splice site false; U1 binds to 5
splice site
c. The formation of the spliceosome brings the 5 and 3
splice sites near each other
d. A lariat is formed in the intron.
32.
Which of the following is NOT true of the lac and ara
operons?
a. They are both regulated by activators and repressors
b. Their respective repressors have two DNA binding sites and
can cause loops to form in the DNA yes; lac has 2
operators and ara has I1, I2, and O2
35.
When Jacob and Monod screened for repressor mutants
E.coli, what did they put in their media?
a. Glucose didnt use lactose as sugar source bc needed it
to not be an inducer
b. X-GAL turned blue when broken down by B-gal
c. IPTG no, would act as an inducer which they didnt want
d. a and b
e. a and c
36.
Experiments not described in class showed that when a
eukaryotic mRNA was hybridized to its genomic DNA, regions
were observed that looped out (were not complementary). This
result is cartooned below.
1
2
a.
b.
c.
d.
translation
translocation
transformation
transcription
Wobble Rules