ActiveCount Manual Rev.1.4

ActiveCount
MICROBIOLOGICAL
AIR SAMPLER
USER MANUAL
ActiveCount 60
Active Count 90-C
Lighthouse ActiveCount User Manual 10/07 rev.1.0
page 1
ActiveCount
60/90-C
Contents
INDEX
page.
GENERAL INFORMATION
Working principle
page.
page.
3
3
DESCRIPTION OF THE UNIT

Case content
Main features
Technical data
Back control panel
page.
page.
page.
page.
page.
4
4
4
5
5
OPERATIVE INSTRUCTIONS
Preparing the unit
Placing the plate
Setting a sampling program
Function Sampling
Run a manual sampling
Run a programmed sampling
Run a sequential sampling
Stored advise
End of sampling
Disinfecting of the unit
Sampling head fire disinfecting
page.
page.
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page.
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page.
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page.
6
6
6
7
7
8
9
11
14
14
15
15
OTHER FUNCTIONS
View battery level
Reset system
Setup head
Setup language
Flow rate calibration
Delete memory
Download data
Stored samplings
Setup date and clock
page.
page.
page.
page.
page.
page.
page.
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16
17
18
18
20
20
20
21
21
22
POWER SUPPLY
page.
23
REMOTE CONTROL
page.
24
SERVICE
page.
25
TROUBLE SHOOTING
page.
25
WARRANTY
page.
26
CONVERSION TABLE ActiveCount 60
page.
27
page.
28
CERTIFICATES
page.
29
page 2
GENERAL INFORMATION
ACTIVECOUNT has been developed for monitoring of air biocontamination level in critical places
(e.g.: clean rooms, food plants, fermentations plants, etc.). The equipment conforms to the new
European ISO/TC 209 dated 25 July 1999 recommendation.
ACTIVECOUNT allows collection of a wide range of airborne biocontaminant by impacting on agar
standard plates (60 mm + 2 mm and 90 mm + 2 mm diameter, filled with every kind of culture
media).
ACTIVECOUNT can be used successfully in a wide range of applications (air quality control in
pharmaceutical, food, cosmetics plants, in hospitals, clinics and laboratories) for sterile or non
sterile areas monitoring. It is very useful also for air monitoring in building air conditioning (HVAC)
plants.
Working principle
The air to be sampled is drawn through a perforated head, at constant velocity and for a period of
time depending on the condition of the area to be controlled. The inflow air impacts in the surface of
the culture media, chosen on the basis of the microbiological investigation to be carried out, placed
in a 90 mm Petri dish. At the end of the sampling period the Petri dish is removed and placed into
an incubator. At the end of the incubation it will be possible to count the CFU/cm2 and evaluate the
air biocontamination level of the critical area on the basis of air volume sampled .
page 3
DESCRIPTION OF THE UNIT

Case content
ACTIVECOUNT air sampler kit includes:
Carrying case
Microbiological sampler "ActiveCount"
60 mm aluminium adding head (inside kit
ActiveCount 60 , cod. G.1006_L )
90-C mm aluminium adding head (inside kit
ActiveCount 90-C, cod. G.1009_L)
60 or 90 mm aluminium head assembly (inside kit
ActiveCount 60/90-C, cod. G1069_L )
Battery charger with full charge indicator
I.R. remote control
Pencil torch
User manual in English
Calibration Report
Main features
Used with: contact plates 60 + 2 mm (ActiveCount 60)
Petri plates 90 + 2 mm (ActiveCount 90C)
the same instrument allows to sample with 60 or 90 mm plates (ActiveCount 60/90C)
Rechargeable battery operated. Battery Ni/Mh (memory effect free)
Silent electric fan microprocessor controlled to assure the maximum reliability of the air volume
sampled
Compensation airflow system (models compensated)
Low battery control and alarm
User friendly. Prompts in 5 languages: English, French, German, Spanish and Italian. All
functions are visualised step-by-step.
Delay start
Total volume of sampled air programmable from 1 up to 2000 litres, with 1 litre steps
Manual sampling (for air volumes greater than 2000 litres)
Sequential sampling also available
Storing and visualising up to 99 samplings, with record of progressive number, date, hour,
volume or time of sampling, flow rate, type of sampling (manual, sequential, programmable).
USB port to download data to a PC (optional)
Calibration control function (with optional calibration kit)
Remote switch with infra-red rays control
Two sampling positions (90-180) and built-in soc ket for tripod
page 4
Technical data
Dimensions:
Weight:
Case:
60 mm head:
90 mm head:
Plate support:
310 x 130 x 170 mm (W x D x H)

1,9 Kg
Polyurethane, joint-free, easy to disinfect
Autoclavable anodised aluminium head, with 219 holes ( 1 mm)
Autoclavable anodised aluminium head, with 380 holes ( 1 mm)
Autoclavable anodised aluminium plate support for 60 and 90 mm 2 mm
plates.
Flow rates:
30 60 90 - 100 (ISO/TC 209) -120 l/min
Sampled Air Volume: 1 - 2000 litres (and more with manual control)
Back control panel
Fig. 1 - Control panel
ON/OFF button
This is the power switch.
Put in the ON position to power the unit.
Place on OFF at the end of the use to save the battery charge.
Data Output
USB port (optional) to transfer on the PC the stored data
Battery charger connector
Remote control infra-red sensor
Display
High contrast graphic display matrix
Start/Stop; Prog; Clear; Set ; Enter ; Up ; Down.

Programming multifunctional keys
page 5
OPERATIVE INSTRUCTIONS
Preparing the unit
Before to start the sampling, ACTIVECOUNT must be disinfected, programmed, loaded with a
plate and put on a flat surface or on a suitable photographic tripod (avoid to screw too hard).
WARNING: dont screw too hard the tripod to avoid demage the
ACTIVECOUNT allows to operate with 60 + 2 mm or 90 mm + 2 mm AGAR PLATES. This it
depends on the head assembly chosen.
First to operate its necessary to set the right plate diameter of the aluminium plate support. This
setting will not be modified until the change of the type or brand of Petri dishes used for the
sampling.
Plate holders
Screw
Aluminium plate
support
Screw
Placing the plate

Wear sterile gloves. Remove the sampling head handling with care to avoid any contamination of
its surfaces. Deposit the head on a clean surface.
Disinfect the aluminium plate support with a suitable disinfectant, avoiding liquid spillage inside the
instrument.
Place a sterile filled plate on the first aluminium plate
support. Press gently in outer direction and lay the
plate on the others (see figure).Remove the dish lid,
and deposit it on a clean surface.
Close the sampling unit screwing clockwise the
sterilised aluminium head on the plate support.
page 6
Setting a sampling program

Switch on the unit by pressing ON push button.
On the display will appear sequentially the following start information:
LIGHTHOUSE
Worlwide
Solution
ActiveCount 90
Version
28/12/06
2.0
12:00
Level
100%
Set
Setup
Prg
Program
Start
Start
FUNCTION: "Sampling"
Press [Start] key to start a sampling using the last stored parameters.
WARNING: using this procedure the delay start is not considered

Press [Prog] key to enter a new sampling program.
Press [Up] and [Down] key to display the following indications :
Samp lin g s t yp e:
M an u al
Pro g rammed
Seq u en t ial
ch an g e
ch an g e
ch an g e
En t er
En t er
En t er
select
select
select
When the needed sampling is shown, press [Enter].
page 7
Run a Manual sampling

The operator can run a manual sampling with times or volumes different from the preset by the
manual equipment control.
To program the ActiveCount on a manual sampling, display:
Samp lin g t yp e:
M an u al
ch an g e
En t er
select
and Press [Enter] key.

It will appear:
F lo w Rat e
xxx l/min
ch an g e
En t er
Where xxx is the last selected flow rate.
select
If the flow rate is correct, press [Enter]. If not, select the needed flow rate using the [Up] and
[Down] keys. When the flow rate needed is displayed press [Enter].
It will appear:
M an u al Samp .
xxx l/min
En t er
select
Where:
Manual Samp.= manual sampling selected
xxx is the needed flow rate.
If the programmed data sampling are correct, press [Enter] and it will appear the sequence of the
three initial displays. If it is necessary to change some data, press the [Clear] key and the first
display (Manual sampling) will appear.
Press [Start] key to start the sampling. The two red LED will switch on and, on the display, it will
appear:
M an u al samp lin g
Where:
t h h : mm: ss
t = sampling time
L= sampling volume
L xxxx.x
F r xxx
Fr = selected flow rate
Press [Stop] key to end the sampling. The two red LED will switch off and, on the display, it will
appear the following message:
Samp lin g en d
F r xxx t h h : mm: ss
En t er esc
Clear d elet e
A beep will continue until it will not be pressed [Enter].
page 8
Run a programmed sampling

To program the ActiveCount on a programmed sampling, display:
Samp lin g t yp e:
Pro g rammed
ch an g e
En t er
select

It will appear:
Vo lu me
L xxxx
ch an g e
En t er
Where xxxx is the last selected volume.
select
If the volume is correct, press [Enter]. If not, select the needed volume using the [Up] and [Down]
keys, to increase or decrease the value, and the [Set] key to move the cursor.
WARNING: Programmed mode allows to sample air volume between 1 and 2000
Lt.. To sample greater air volumes it is necessary to work in Manual
mode.
When the volume needed is displayed press [Enter].
It will appear:
F lo w Rat e
xxx l/min
ch an g e
En t er
Where xxx is the last flow rate selected.
select
It will appear:
Delay St art
00 : 00
ch an g e
En t er
select
Set the needed delay using the [Up] and [Down] keys, to increase or decrease the value, and the
[Set] key to move the cursor. When the delay needed is displayed press [Enter].
page 9
WARNING: Set EXACTLY delay time (hour an minutes) for the beginning of the
sampling
If the delay start is not needed, leave 00:00
After the [Enter] key has been pressed, all the parameters will be displayed (Volume, Flow rate,
Sampling time calculated on the volume and flow rate basis, and the eventual delay start)
F r xxx l/min
V
yyyy L
Ds 00 : 00
t
mm : ss
Where:
Fr = set flow rate (L/min.)
V = sampling volume (Litres)
Ds = delay start
t
= sampling duration (the sampling time calculation
is based on the flow rate and volume set)
If the set data are correct, press [Enter] to save the program.
The three first displays will appear.
If it is necessary to change one or more data, press the [Clear] key and it will be shown the initial
display with the programmed sampling settings. Press the [Enter] key up to the parameter that it is
necessary to change. Modify the parameter and press [Enter] to save the setting. Press [Enter]
up to the summing up screen and save the set parameters pressing the [Enter] key.
The three initial displays will appear and the ActiveCount is ready for the sampling.
a) The delay start is not active:
The two red LED will brighten and, on the display, will be shown:
Pro g ram samp lin g
Pro g ram
samp lin g
and then
w ait
t h h : mm: ss
L xxxx.x
F r xxx
st art
At the end of the sampling period the red LED will go out and the following message will
appear:
Samp lin g en d
F r xxx t h h : mm: ss
En t er esc
Clear d elet e
At the same time a beep will inform the user that the sampling is finished. Press [Enter]
key to switch off the acoustic alarm. The initial displays will appear.
b) The delay start is activated
The two red LED will lighten and, on the display, will be shown:
W ait p lease
Delay St art
h h : mm : ss
page 10
At the end of the delay start the LED will brighten and, on the display, will be shown:
Pro g ram
samp lin g
Pro g ram samp lin g

and then
w ait
t h h : mm: ss
L xxxx.x
F r xxx
st art
At the end of the sampling period the red LED will go out and the SAMPLING END
message will appear.
At the same time a beep will inform the user that the sampling is terminated. Press [Enter]
key to switch off the acoustic alarm and the three initial displays will appear.
Run a sequential sampling

To program the ActiveCount on a sequential sampling, display:
Samp lin g t yp e:
Seq u en t ial
ch an g e
En t er
select

It will appear:
Vo lu me
L xxxx
Where xxxx is the last selected volume.
ch an g e
En t er select
If the volume is correct, press [Enter]. If not, select the needed volume using the [Up] and [Down]
keys, to increase or decrease the value, and the [Set] key to move the cursor.
WARNING: The Seqential mode allows ONLY samplings with air volume between
1 and 2000 litres
When the needed volume is displayed press [Enter] key.
It will appear:
F lo w Rat e
xxx l/min
ch an g e
Where xxx is the last flow rate selected.
En t er select
page 11
It will appear:
NUM SQ 01
Where:
BreakSQ
NUM SQ = the number of sequential samplings

(from 1 up to 99 cycles)
BreakSQ = the interval (minutes) between two
samplings (the maximum allowed is
120 min.)
001 min
ch an g e
En t er select
Set the needed data using the [Up] and [Down] keys, to increase or decrease the value, and the
[Set] key to move the cursor.
WARNING: It is possible to set from 1 up to 99 cycles

It is possible to se the delay time between 1 and 120 minutes max.
When the sequence needed is displayed press [Enter].
It will appear:
Delay St art
00 : 00
ch an g e
En t er select
Set the needed delay start for the first sampling using the [Up] and [Down] keys, to increase or
decrease the value, and the [Set] key to move the cursor. When the delay needed is displayed
press [Enter].
WARNING: Set EXACTLY delay time (hour an minutes) for the beginning of the
sampling
If the delay start is not needed, leave 00:00
After the [Enter] key has been pressed, all the parameters will be displayed (Flow rate, volume, the
eventual delay start, sampling time calculated on the volume and flow rate basis, number of setting
sequences, time between sequences).
F r xxx l/min
V yyyy
Ds 00: 00
t mm: ss
Nseq n n
Bseq sss
Where:
xxx
yyyy
Ds 00:00
t mm:ss
Nseq nn
Bseq sss
= set flow rate (L/min.)

= sampling volume (Litres)
= Delay start
= sampling duration in seconds (the sampling time calculation is based
on the flow rate and volume set)
= number of sequences
= interval between a sampling and the following (seconds)
If the set data are correct, press [Enter] to save the program.
page 12
The two first displays will appear.

If it is necessary to change one or more data, press the [Clear] key and the it will be shown the
initial display with the programmed sampling settings. Press the [Enter] key up to the parameter
that it is necessary to change. Modify the parameter and press [Enter] to save the setting. Press
[Enter] up to the summing up screen and save the set parameters pressing the [Enter] key.
The two initial displays will appear and the ActiveCount is ready for the sampling.
Press [Start] and the ActiveCount will start the sampling as shown for the programmed sampling.
It will appear:
Seq u en t ial
samp lin g
w ait
st art
During sampling:
Seq u en . samp lin g
t h h : mm: ss
L xxxx.x
Sq 01/NN
Where:
t
= countdown end sampling (h/m/s)
L
= sampling volume (Litres)
Sq = sampling number respect the numbers
sampling set (es : 01/02 says that we are
doing the first sampling up two)
In this moment the LED will switch on.

Between two sequences sampling:
t h h : mm: ss
Next samp lin g
Sq n n /NN
Where:
Sq nn/NN = n sampling to do over the total
sampling to do (es : 02/02 says that we
are waiting for beginning the second
sampling up two)
t = countdown end sampling (h/m/s)
In this moment the LED will brighten.

During second sampling:
t h h : mm: ss
L xxxx.x
Where the display is the same than first sampling

except the n of sequence
Sq 02/NN
In this moment the LED will switch on.

The same previous displays will be shown after this point until the end of last sampling set. At the
end of the sampling period the red LED will go out and the it will appear:
page 13
Samp lin g en d
F r xxx
Sq
n n /NN
En t er esc
At the same time a beep will inform the user that the sampling is terminated. Press [Enter] key to
switch off the acoustic alarm and the three initial displays will appear.
Stored advise
When the stored memory is almost full, when it is arrived to 150 up to 160 of storage sampling, it
will appear the following advise:
M emo ry
almo st f u ll
Co n t in u e?
ENT yes CL EAR n o
When the stored memory is full, it will appear the following advise:
M emo ry
f u ll
Co n t in u e?
ENT yes CL EAR n o
If yes is choosen, the sampling will be overwritten to the previous sampling.
If 2no2 is choosen , the sampling will be not memorized.
End of sampling
Switch off the ActiveCount pressing the [Off] key.
Remove the sampling head unscrewing anticlockwise.
Close the plate with the lid, and after the identification, bring it to incubation.
Prepare the unit to the next sampling.
WARNING: In order to save energy when ACTIVECOUNT is waiting for a new

sampling, after about 120 seconds of its inactivity the LCD display
lighting system automatically switch off until ENTER key is pressed.
page 14
Disinfecting of the unit

ACTIVECOUNT must be cleaned after each cycle to avoid cross contamination which would modify
the reliability of the sampling.
Switch off the unit.
Clean the surface of ACTIVECOUNT using a sterile cloth wet with a suitable disinfectant. After
drying, the unit is ready for a new sampling cycle.
WARNING: DONT SPRAY disinfectant solution directly on the instrument or the

keyboard: these solutions could enter inside the instrument and they
cause damage as example and oxidation of the circuits.
LIGHTHOUSE WILL NOT RECOGNIZE THE WARRANTY CAUSED OF
UNSUITABLE DISINFECTANT SOLUTION
The sampling head can be autoclaved in an autoclavable bag, following the standard procedure of
sterilisation ( i.e. 134 C, 18 minutes for hospita ls applications).
Between two samplings is necessary to disinfect the sampling head with pencil torch supplied with
the starting kit. In the absence of this, it is possible to use a sterile cloth with disinfectant over this. It
is very important to avoid the liquid penetration inside the instrument.
Let dry at room temperature. After drying the sampling head is ready to use.
Sampling head fire disinfecting

The ActiveCount kit is equipped with a pencil to disinfect the head. Disinfect always the head
between two samplings to avoid a cross-contamination.
PENCIL
Head
TORCH
Control - valve
To switch on the pencil torch rotate clockwise the control-valve. When the gas goes out
from the head, bring the fire to light the pencil.
WARNING: Pass quickly the fire on the interior part of the sampling head.
Do not stop the fire on a specific point of the head, it can be damaged.
To switch off the pencil torch rotate counter-clockwise the control-valve.
To recharge the pencil torch it is possible to use the Butan gas used for cigarette-lighters.
Before to recharge the pencil torch be sure it is switched off. The complete recharge need about
10 seconds. It is necessary to wait some minute the gas is stable.
For correct recharging it is recommended to keep the pencil torch horizontal and the recharge
cartridge
page 15
OTHER FUNCTIONS
Press [o/off] to switch on the instrument.
Press [Set] to enter in the setting menu of the ACTIVECOUNT.
Press the [Up] key to scroll the following menus on the Display:
Bat t ery lev el
ch an g e
En t er
select
St eu p
d at e an d clo ck
ch an g e
Reset Syst em
En t er
select
ch an g e
En t er
select
L an g u ag e Set u p
St o red samp lin g
ch an g e
En t er
select
ch an g e
En t er
select
F lo w rat e
calib rat io n
Delet e memo ry
ch an g e
En t er
select
ch an g e
En t er
select
Press [Enter] to enter in a menu.

When inside the instrument is fitted the download data system (cod. G1117_L), after the display
Delet e memo ry
ch an g e
En t er
select
It will appear:
Do w n lo ad ed d at a
ch an g e
En t er
select
page 16
In the ActiveCount 60/90-C (cod. G.1069_L), and ONLY in this instrument, after the display
Set u p
L an g u ag e
ch an g e
En t er
select
It will appear:
Set u p h ead
ch an g e
En t er
select
FUNCTION: "Battery level"

In the battery level menu:
Bat t ery L ev el
ch an g e
En t er
select
Press [Enter] and it will appear while the fun switch on:
L ev el HIG H
100%
The battery level is done by the number (%) showed on the display and from the HIGH
WARNING and LOW indications.
WARNING indication it will appear when the level arrives to 30%, while LOW indication it will
appear when the level arrives to 5%.
Press the [Clear] key to exit from the Battery level menu.
Press one time the [Clear] key to stay in the Set menu. Press two times the [Clear] key to visualise
the first three displays.
page 17
FUNCTION: "Reset system"

In the rest system menu:
Reset Syst em
ch an g e
En t er
select
Press [Enter] after the visualisation and it will appear:

Passw o rd
0000
This menu can be only used by the manufacturer.

Press two times the [Enter] key to visualise the first three displays.
FUNCTION: "Setup head"

(only for ActiveCount 60/90-C)
In the Setup head menu:
Set u p h ead
ch an g e
En t er
select

Head
90
ch an g e
En t er
select
Press the [Up] and [Down] keys to scroll the available heads: 60 and 90.
Press [Enter] and the following display will appear:
Are yo u su re ?
No
page 18
Press the [Up] and [Down] keys to choose yes or no and confirm with [Enter] key.
The Setup head menu will be displayed again. Press the [Clear] key to come back to the main
menu.
FUNCTION: " Language Setup "

In the Language Setup menu:
ch an g e
En t er
select
Press [Enter] after the visualisation and It will appear:

ENG L ISH
Press the [Up] and [Down] keys to scroll the available languages (ITALIANO, ENGLISH,
FRANCAIS, DEUTSCH, ESPANOL).
Press [Enter] to confirm.
After the confirmation, it will appear automatically the set menu. Press the [Clear] key to visualise
FUNCTION: "Flow rate calibration"

In the Flow rate calibration menu:
F lo w rat e
calib rat io n
ch an g e
En t er
select
Press [Enter] after the visualisation and It will appear:

Passw o rd
0000
page 19
In order to avoid an undesired cancellation of the calibration, the password

will be supplied ONLY at the customers who buy the ActiveCount calibration
kit.
Press the [Enter] key to exit from the Flow rate calibration menu.
Press one time the [Enter] key to stay in the Set menu. Press the [Clear] key to visualise the first
three displays.
FUNCTION: "Delete memory"

In the Delete memory menu
Delet e memo ry
ch an g e
En t er
select

Are yo u su re ?
No
If it is not necessary to delete the stored data press the [Enter] key to exit from this menu.
If it is necessary to delete the data press the [Down] key and it will appear:
Are yo u su re ?
Y es
Press the [Enter] key to delete all the stored data and it will appear
Erase
memo ry
After waiting the time for the memory reset, it will appear automatically the set menu. Press the
[Clear] key to visualise the first three displays.
page 20
FUNCTION: "Download data "

In the Download data menu:
W ait p lease
Do w n lo ad d at a
T x 002/002

Do w n lo ad
Dat a
ch an g e
En t er
select
After the download it will appear automatically the set menu. Press the [Clear] key to visualise the
first three displays.
NOTE: during this step, the ActiveCount has to be connected to a PC with the proper cable. We
suggest to download only after the connection with the PC.
WARNING: Every time the download is done, the ActiveCount transfer all the data
to the PC. We suggest to erase the data stored on the ActiveCount after
the download.
FUNCTION: "Stored samplings"

In the Stored samplings menu:
St o red samp lin g
ch an g e
En t er
select
Press [Enter] after the visualisation an it will appear:

a) Manual and Programmed sampling
N xxx
d d /mm/yy h h : mm
F ryyy l/min H 90
t wwww s
Where:
Nxx
dd/mm/yy
hh:mm
Fr = yyy
H
t= wwww
= number of sampling
=date (day/month/year)of the sampling
= time (hour/minutes) of the sampling
= set flow rate (L/min)
= head
= duration of the sampling (seconds)
page 21
b) Sequential sampling
N xxx Seq 01/02
d d /mm/yy h h : mm
F ryyy l/min H 90
V zzzz L
Where:
Nxx
dd/mm/yy
hh:mm
Seq
Fr=yyy
H
V=vvvv
01 of 02
02 of 02
= number of sampling
= date (day/month/year) of the sampling
= time (hour/minutes) of the sampling
= sequential sampling
= set flow rate (L/min)
= head
= air volume sampled (Litres)
= the first sampling of a sequence of 2 samplings
= the second sampling of a sequence of 2 samplings
Press the [Clear] key to exit from the Stored samplings menu.
Press one time the [Clear)] key to stay in the Set menu. Press two times the [Clear] key to
visualise the first two displays.
FUNCTION: "Setup date and clock"

In the Setup date and clock menu,
St eu p
d at e an d clo ck
ch an g e
En t er
select

Dat a
25/02/07
Ho u r
12: 42: 03
ch an g e
En t er select
Set data and time using the [Up] and [Down] keys, to increase or decrease the value, and the
[Set] key to move the cursor.
Press the [Clear] key to exit from the Setup date and clock menu.
Press one time the [Clear] key to stay in the Set menu. Press two times the [Clear] key to visualise
page 22
POWER SUPPLY
The unit is powered by a 8 elements Ni/MH battery pack (each element: 1,2V) protect against
short-circuits and thermal overcharges.
NOTE: This battery pack is memory effect free and it can be recharged when needed without
damages.
To charge the battery pack connect the battery charger to the main power and put the battery
charger plug into the socket places on the back panel of the unit.
Charge the battery pack when the unit is turned off.
With a completely discharge battery pack, the recharging time will take about 4 5 hours.
When you connect the battery charger with the power, the green LED switch on.
The following table reassume the various recharge condition when you connect the battery pack to
the charger:
BATTERY
STATUS
Full
In charge
Low
Empty
LED
Fixed yellow
Flashing yellow
Flashing yellow
Fixed red
Flashing yellow
Switch off yellow led
Switch off yellow led
Fixed yellow
Flashing yellow
Switch off yellow
If the battery charge level cannot assure the programmed flow rate the sampling is automatically
stopped and it will be saved with the sampling time.
If necessary, the battery pack can be easily replaced removing the bottom aluminium panel of the
unit. Disconnect the connector detach from Velcro band and replace the battery pack.
When the instrument is switched on and it doesnt work for 10 minutes, to preserve the battery
pack, it goes in STAND BY (it will appear an indication on the display) .
ActiveCount has a microchip that avoids the beginning of a sampling if the battery level is not
enough to maintain a constant flow rate for all the sampling time. So if the battery level will
decrease under the WARNING limit, it will appear:
Bat t ery
Level
VERY LOW
W AR N I N G
When the this indication appears, the instrument will work only for 4 sampling. After last sampling
the instrument switchs off.
WARNING: It will be not memorized the programmed sampling but only the
effective sampling time.
page 23
Following table shows the battery life if the instrument is used with only a flow rate for all the time of
the sampling. If you work with different flow rates this table in not valid anymore.
FLOW
RATE
30 Lt
60Lt
90 Lt
100 Lt
120 Lt
LIFE
BATTERY
10 h
8h
7h
6h
5h
REMOTE CONTROL
ACTIVECOUNT is supplied with a remote control that allows
the operator to start and stop the instrument away from the
point of sampling.
Keep pressed the button for some second to start or stop
the sampling.
To change the internal battery, open the small frontal panel.
Button
Frontal panel
page 24
SERVICE
All operations shall be performed by qualified personnel only!
There are no parts replaceable by the user himself. In case of problems please call your dealer for
service.
TROUBLE SHOOTING
The unit is off
Verify:
1.
The unit is switch on
2.
The battery is charged
3.
If doesnt work again, please call for service.
The blower motor does not work
Please call for service
The infrared remote control switch doesnt

start the sampling
Verify:
1.
Infrared remote switch battery is charged
2.
If doesnt work again, please call for service.
LCD display doesnt work properly
Please call for service.
LCD display shows question marks or other

characters
Red LED doesnt light during the sampling
Blower motor make noise
Verify:
Fan velocity is out of any control
Soft touch control panel doesnt work.
1.
Presence of foreign bodies inside the fan
2.
If noise still persists, please call for service
1.
If the problem is present for a flow rate only,

calibrate that flow rate with the calibration kit
following related instructions.
2.
If the problem is present for all flow rates,

verify each flow rate calibration
3.
If the model is 60/90, take off the head and

the support plate. Verify the alignment of the
fan
4.
If the problem still persists, please call for

service.
page 25
WARRANTY
Lighthouse provides 1 year warranty on the instrument starting on the date of purchase.
Within the warranty period Lighthouse will eliminate, free of charge, any defects in the
ACTIVECOUNT microbiological air sampler resulting from failures in material or workmanship
either by repairing, or exchanging parts or exchanging the whole appliance as Lighthouse may
choose.
Damages caused by improper use as well as negligence to use the instrument, normal
deterioration of internal parts , some defects with negligible effects on the routinely function of the
instrument are not included in this warranty.
This warranty does not apply in case of technical intervention repairs which are carried out by non
authorized personnel.
Lighthouse is not ever responsible, in front of the purchaser or end user, for damage caused by
people or things (included the same instrument) in relation to the functioning of the instrument, that
it is caused by:
a) any type of work or modification carried out inside the instrument unit, or its accessories,
without Lighthouse previous authorization and /or Lighthouse technical direct instructions,
even in the case that such works are carried out by Lighthouse employees, but without
Lighthouse proper authorization.
b) to use the ACTIVECOUNT and/or its accessories for purposes which are different from the
original purposes indicated by Lighthouse at the time of planning the design, manufacture
and sell the instrument
c) to clean ACTIVECOUNT and/or its accessories with chemical products different from the
Lighthouse indications and /or to use ACTIVECOUNT under different operational
conditions as indicated by Lighthouse
For further information concerning ActiveC

ctiveCount please contact:
Lighthouse Worldwide Solutions Benelux BV. Van Heemstraweg 19a

NL-6657 KD Boven-Leeuwen, The Netherlands
Tel +31-487-560811 - FAX +31-487-560013
E-mail: Benelux@golighthouse.com
For technical assistance please contact:
Lighthouse Worldwide Solutions Benelux BV. Van Heemstraweg 19a

NL-6657 KD Boven-Leeuwen, The Netherlands
Tel +31-487-560811 - FAX +31-487-560013
Lighthouse ActiveCountE-mail:
User Manual
Benelux@golighthouse.com
10/07 rev.1.0
page 26

To correlate the CFUs (Colony Forming Units) present on the agar plate to the most probable
number (MPN) of micro-organisms per cubic meter of air sampled, the following formula is used:
Pr = N [1/N + 1/(N-1) + 1/(N-2) + ... + 1/(N-r+1)]
Where:
Pr = most probable number of micro-organisms in the volume of air sampled
N = number of holes on ActiveCount sampling head
R = number of CFUs on the agar plates after incubation
r
r
1
30
P
r
32
59
P
r
69
88
P
r
112
117
P
r
167
146
P
r
240
175
P
r
349
204
P
r
580
31
33
60
70
89
114
118
169
147
243
176
354
205
595
32
35
61
71
90
116
119
171
148
246
177
360
206
611
33
36
62
73
91
117
120
173
149
249
178
365
207
628
34
37
63
74
92
119
121
175
150
252
179
370
208
646
35
38
64
75
93
121
122
178
151
255
180
376
209
666
36
39
65
77
94
122
123
180
152
258
181
381
210
688
37
40
66
78
95
124
124
182
153
261
182
387
211
712
38
42
67
80
96
126
125
185
154
265
183
393
212
739
10
10
39
43
68
81
97
128
126
187
155
268
184
399
213
771
11
11
40
44
69
83
98
130
127
189
156
272
185
405
214
807
12
12
41
45
70
84
99
131
128
192
157
275
186
412
215
851
13
13
42
47
71
86
100
133
129
194
158
279
187
418
216
906
14
14
43
48
72
87
101
135
130
196
159
282
188
425
217
979
15
16
44
49
73
89
102
102
131
199
160
286
189
432
218
1.088
16
17
45
50
74
90
103
139
132
201
161
290
190
440
219
1.307
17
18
46
52
75
92
104
141
133
204
162
293
191
447
18
19
47
53
76
93
105
143
134
206
163
297
192
455
19
20
48
54
77
95
106
144
135
209
164
301
193
463
20
21
49
55
78
96
107
146
136
212
165
305
194
471
21
22
50
57
79
98
108
148
137
214
166
309
195
480
22
23
51
58
80
99
109
150
138
217
167
313
196
489
23
24
52
59
81
101
110
152
139
220
168
317
197
499
24
25
53
61
82
102
111
154
140
222
169
322
198
509
25
26
54
62
83
104
112
156
141
225
170
326
199
519
26
27
55
63
84
106
113
158
142
228
171
331
200
530
27
29
56
65
85
107
114
160
143
231
172
335
201
542
28
30
57
66
86
109
115
163
144
234
173
340
202
554
29
31
58
67
87
111
116
165
145
237
174
345
203
567
page 27

To correlate the CFUs (Colony Forming Units) present on the agar plate to the most probable
number (MPN) of micro-organisms per cubic meter of air sampled, the following formula is used:
Pr = N [1/N + 1/(N-1) + 1/(N-2) + ... + 1/(N-r+1)]
Where:
Pr = most probable number of micro-organisms in the volume of air sampled
N = number of holes on ActiveCount sampling head
R = number of CFUs on the agar plates after incubation
r
r
r
r
r
P
r
P
r
P
r
P
r
P
r
46
49
91
104
136
168
181
245
226
342
271
473
316
674
361
1129
47
50
92
105
137
170
182
247
227
345
272
477
317
680
362
1149
48
51
93
106
138
171
183
249
228
347
273
480
318
686
363
1170
49
52
94
108
139
173
184
251
229
350
274
484
319
693
364
1192
50
54
95
109
140
174
185
253
230
352
275
487
320
699
365
1216
51
55
96
110
141
176
186
255
231
355
276
491
321
705
366
1242
52
56
97
112
142
178
187
257
232
358
277
495
322
712
367
1269
53
57
98
113
143
179
188
259
233
360
278
498
323
718
368
1298
54
58
99
115
144
181
189
261
234
363
279
502
324
725
369
1330
10
10
55
59
100
116
145
182
190
263
235
365
280
506
325
732
370
1364
11
11
56
60
101
117
146
184
191
265
236
368
281
510
326
738
371
1402
12
12
57
62
102
119
147
186
192
267
237
371
282
514
327
745
372
1444
13
13
58
63
103
120
148
187
193
269
238
373
283
517
328
753
373
1492
14
14
59
64
104
121
149
189
194
271
239
376
284
521
329
760
374
1546
15
15
60
65
105
123
150
190
195
273
240
379
285
525
330
767
375
1609
16
16
61
66
106
124
151
192
196
275
241
381
286
529
331
775
376
1685
17
17
62
68
107
125
152
194
197
277
242
384
287
533
332
783
377
1780
18
18
63
69
108
127
153
195
198
279
243
387
288
537
333
791
378
1907
19
19
64
70
109
128
154
197
199
281
244
390
289
542
334
799
379
2097
20
21
65
71
110
130
155
199
200
283
245
392
290
546
335
807
380
2477
21
22
66
72
111
131
156
200
201
285
246
395
291
550
336
815
22
23
67
74
112
132
157
202
202
288
247
398
292
554
337
824
23
24
68
75
113
134
158
204
203
290
248
401
293
559
338
833
24
25
69
76
114
135
159
206
204
292
249
404
294
563
339
842
25
26
70
77
115
137
160
207
205
294
250
407
295
567
340
851
26
27
71
78
116
138
161
209
206
296
251
410
296
572
341
861
27
28
72
80
117
140
162
211
207
298
252
413
297
576
342
871
28
29
73
81
118
141
163
213
208
301
253
415
298
581
343
881
29
30
74
82
119
143
164
214
209
303
254
418
299
586
344
891
30
31
75
83
120
144
165
216
210
305
255
421
300
590
345
901
31
32
76
85
121
145
166
218
211
307
256
425
301
595
346
912
32
33
77
86
122
147
167
220
212
310
257
428
302
600
347
923
33
34
78
87
123
148
168
221
213
312
258
431
303
605
348
935
34
36
79
88
124
150
169
223
214
314
259
434
304
610
349
947
35
37
80
90
125
151
170
225
215
316
260
437
305
615
350
959
36
38
81
91
126
153
171
227
216
319
261
440
306
620
351
972
37
39
82
92
127
154
172
229
217
321
262
443
307
625
352
985
38
40
83
94
128
156
173
230
218
323
263
447
308
630
353
998
39
41
84
95
129
157
174
232
219
326
264
450
309
635
354
1012
40
42
85
96
130
159
175
234
220
328
265
453
310
641
355
1027
41
43
86
97
131
160
176
236
221
330
266
456
311
646
356
1042
42
44
87
99
132
162
177
238
222
333
267
460
312
652
357
1058
43
46
88
100
133
163
178
240
223
335
268
463
313
657
358
1075
44
47
89
101
134
165
179
242
224
338
269
466
314
663
359
1092
45
48
90
103
135
167
180
243
225
340
270
470
315
669
360
1110
page 28
The Company
Lighthouse Worldwide Solutions Benelux BV.
Van Heemstraweg 19a, NL-6657 KD Boven leeuwen The Netherlands
in the person of President and Legal representative
Martin Derks
Declares that
the Active
ctiveC
Count microbiological air sampler
Serial Number N .
conforms to the following E.C. standard
Immunity:
IEC 1000 4- 3
IEC 1000 4 2
(R.F.E.M. Field)
(Electrostatic Discharge)
Emission:
EN 55011
EN 55011
(Conducted Emission)
(Radiated Emission Pre-Compl.)
Others:
CEI EN 50082 - 2
CEI EN 50081 - 1
(Generic Immunity Std)

(Generic Emission Std)
Alphen aan de Maas, 27 October 2009
Lighthouse Benelux BV.

Legal Representative
page 29
Lighthouse Active Count User Manual 10/07 rev.1
page 30

ActiveCount Manual Rev.1.4

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ActiveCount

DESCRIPTION OF THE UNIT

CONVERSION TABLE ActiveCount 60

CONVERSION TABLE ActiveCount 90

Lighthouse ActiveCount User Manual 10/07 rev.1.0

Lighthouse ActiveCount User Manual 10/07 rev.1.0

DESCRIPTION OF THE UNIT

Lighthouse ActiveCount User Manual 10/07 rev.1.0

310 x 130 x 170 mm (W x D x H)

Back control panel

Fig. 1 - Control panel

Battery charger connector

Remote control infra-red sensor

Start/Stop; Prog; Clear; Set ; Enter ; Up ; Down.

Lighthouse ActiveCount User Manual 10/07 rev.1.0

Placing the plate

Lighthouse ActiveCount User Manual 10/07 rev.1.0

Setting a sampling program

WARNING: using this procedure the delay start is not considered

When the needed sampling is shown, press [Enter].

Lighthouse ActiveCount User Manual 10/07 rev.1.0

Run a Manual sampling

and Press [Enter] key.

Where xxx is the last selected flow rate.

Lighthouse ActiveCount User Manual 10/07 rev.1.0

Run a programmed sampling

and Press [Enter] key.

Where xxxx is the last selected volume.

Where xxx is the last flow rate selected.

Lighthouse ActiveCount User Manual 10/07 rev.1.0

Lighthouse ActiveCount User Manual 10/07 rev.1.0

Pro g ram samp lin g

Run a sequential sampling

and Press [Enter] key.

Where xxxx is the last selected volume.

Where xxx is the last flow rate selected.

Lighthouse ActiveCount User Manual 10/07 rev.1.0

NUM SQ = the number of sequential samplings

WARNING: It is possible to set from 1 up to 99 cycles

= set flow rate (L/min.)

Lighthouse ActiveCount User Manual 10/07 rev.1.0

The two first displays will appear.

In this moment the LED will switch on.

In this moment the LED will brighten.

Where the display is the same than first sampling

In this moment the LED will switch on.

Lighthouse ActiveCount User Manual 10/07 rev.1.0

WARNING: In order to save energy when ACTIVECOUNT is waiting for a new

Lighthouse ActiveCount User Manual 10/07 rev.1.0

Disinfecting of the unit

WARNING: DONT SPRAY disinfectant solution directly on the instrument or the

Sampling head fire disinfecting

Lighthouse ActiveCount User Manual 10/07 rev.1.0

Bat t ery lev el

St o red samp lin g

Press [Enter] to enter in a menu.

Lighthouse ActiveCount User Manual 10/07 rev.1.0

FUNCTION: "Battery level"

Lighthouse ActiveCount User Manual 10/07 rev.1.0

FUNCTION: "Reset system"

Press [Enter] after the visualisation and it will appear:

This menu can be only used by the manufacturer.

FUNCTION: "Setup head"