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Experiment Results
Predict Question:
Predict Question: Predict what will happen to the resting membrane potential if the extracellular K+ concentration is
increased.
Your answer : b. The resting membrane potential will become less negative.
Stop & Think Questions:
What is the polarity of the resting membrane potential (voltage)?
Your answer : a. positive
Correct answer: b. negative
What does it mean that the voltage just inside the membrane is negative?
Your answer : c. There are more positive charges than negative charges just inside the membrane.
Correct answer: b. There are more negative charges than positive charges just inside the membrane.
The membrane of most cells, including neurons, contains passive, open,
K+ leak channels. Given the normal K+ concentrations and the resultant concentration gradient, which direction would K+
be expected to move (diffuse) through these leak channels?
Your answer : a. into the cell
Correct answer: b. out of the cell
What effect does increasing extracellular K+ have on the net diffusion of K+ out of the cell?
Your answer : a. It increases the net diffusion of K+ .
Correct answer: b. It decreases the net diffusion of K+ .
Which way would Na+ move across the membrane if there were open Na+ channels?
You correctly answered: a. Na+ would diffuse into the cell.
The membrane has open K+ channels, and changing extracellular K+ concentration results in a change in membrane
potential. Changing the extracellular Na+ concentration does not significantly change the membrane potential. What do
your results suggest about the number or state (open or closed) of Na+ channels in the resting membrane of a neuron?
You correctly answered: b. Na+ channels are mostly closed.
Experiment Data:
Extracellular Fluid (ECF)
Control
Control
Control
Control
High K+
High K+
High K+
High K+
Low Na+
Low Na+
Low Na+
Low Na+
Microelectrode Position
Cell body extracellular
Cell body intracellular
Axon extracellular
Axon intracellular
Axon intracellular
Axon extracellular
Cell body extracellular
Cell body intracellular
Cell body intracellular
Cell body extracellular
Axon extracellular
Axon intracellular
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Voltage (mV)
0
-70
0
-70
-40
0
0
-40
-72
0
0
-72
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