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Figure 1: Changes in the concentration of microbial biomass (), sucrose (), xanthan gum () when the initial sucrose concentration was
25.0 g L-1. Symbols represent the experimental results in kinetic data. (S. Faria et al, 2010)
25
21
15
9
3
0.5
0.25
1.125
2.25
2.5
2.5625
2.5625
0
0.5
2.5
6.5
11.5
13.5
Xs
(Sucrose F(L/hr) CN(g/L) dS/dt=rS dX/dt=rX dP/dt=rP dN/dt=rN
YX/S
YP/S
YP/X
YN/X
Conv)
0
0
0
-0.6
0.15
-0.05
0.2294
0.25
0.08333 0.3333
1.5293
0.16
6250
0.7706
-1
0.2
0.25
0.0788
0.2
0.25
1.2500
0.3942
0.286
1750
0.7884
-1.2
0.1375
0.6
-0.0520
0.11458
0.5
4.3636
0.3780
0.40
833.333 0.2508
-1.2
0.03125
0.9
-0.0670 0.002604
0.75
28.8000 2.1450
0.667
455.357 0.1180
-1.85
0.00625
0.7
0.0585
0.00735 0.82353 112.0000 9.3647
0.833
450
0.8361
-0.15
-0.00625
0.1
0.2287
0.04167 0.66667 16.0000 36.5933
Table 1: Data for calculation of Economic Potential 2
Time
(h)
Yx/s
0.25
0.2
0.25
10
0.114583
15
0.026042
20
25
Yp/s
0.6723
0.2313
11.8551 12.0629
0.0306
10.9080
0.5379
0.1850
9.5299
10.2024
0.0917
0.5
7.0877
0.3082
0.1060
6.0036
7.3362
0.1834
0.75
3.2374
0.0700
0.0241
2.4657
4.4473
0.2752
3.6509
0.3021
5.3096
0.2446
0.0833333 13.3885
Xs =
= 0.40
Interior point =
Final point
Initial point
)
)
)
*Since t=15h is at between 0-25 hours, we will use interior point to calculate.
( )
= -1.2
( )
= 0.03125
( )
= 0.9
|
= 0.026
= 28.8
= -0.75
(
)(
)(
Stoichiometry Calculation at t = 15 h
Based on EP 1:
Elemental balances
C balance
H balance
O balance
N balance
: 370g/mol
: 342.30g/mol
: 933g/mol
From Table 1:
f = 0.2752
c = 0.024
From N balance:
From C balance:
From H balance:
From O balance:
)
(
)
)
Time (h)
0
5
10
15
20
25
H2O
47.57
13.41
4.82
1.95
1.46
2.62
Amount of
Amount of O2
CO2 released
consumed
(kg/h)
(kg/h)
0
114.32
47.57
5
30.63
13.41
10
9.65
4.82
15
2.64
1.95
20
1.42
1.46
25
4.33
2.62
Table 4: Data for gas distribution
Time (h)
Concentration vs Conversion
30
Concentration (g/L)
25
20
Sucrose
15
Biomass
Xanthan
10
Yeast
5
0
0
0.2
0.4
0.6
0.8
Sucrose conversion
*(
)+
Time
(h)
0
5
10
15
20
25
Economic
Economic
Potential 2
Potential 2
(RM/h)
(RM/year)
0.00
0
0.00
0.00
-2900642.68
29.313
391.89
-362.58
-324678.37
41.038
81.62
-40.58
231439.64
50.808
21.88
28.93
360098.05
49.119
4.11
45.01
427559.35
56.984
3.54
53.44
Table 5: Data for Economic Potential calculation
Revenue
Raw
material
XS
(Sucrose
Conv.)
0.00
0.16
0.29
0.40
0.67
0.83
EP 2 vs Conversion
1000000
Economic Potential 2 (RM/year)
EP 2
500000
0
-500000
0.1
0.2
0.3
0.4
0.5
0.6
0.7
-1000000
-1500000
-2000000
-2500000
-3000000
-3500000
Sucrose conversion